A5015955603- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Transplantation: Methods and Outcomes
- Cancer Diagnosis and Treatment
- Renal Transplantation Outcomes and Treatments
- 3D Printing in Biomedical Research
- Infective Endocarditis Diagnosis and Management
- Neutropenia and Cancer Infections
- Cancer Cells and Metastasis
- Cardiac Valve Diseases and Treatments
- T-cell and Retrovirus Studies
- Organ and Tissue Transplantation Research
- Blood transfusion and management
- Platelet Disorders and Treatments
- Immunotherapy and Immune Responses
- Asthma and respiratory diseases
- Neonatal Respiratory Health Research
- Vascular Procedures and Complications
- Infectious Aortic and Vascular Conditions
- Aortic Disease and Treatment Approaches
- Monoclonal and Polyclonal Antibodies Research
University Hospital Centre Zagreb
2013-2024
University of Applied Health Sciences
2018-2019
Klinički Bolnički Centar Rijeka
2009
Due to their ability induce immunological tolerance in the recipient, mesenchymal stromal cells (MSCs) have been utilized treatment of various hematological and immune- inflammation-mediated diseases. The clinical application MSCs implies prior vitro expansion that usually includes use fetal bovine serum (FBS). present study evaluated effect different platelet lysate (PL) media content on biological properties MSCs. were isolated from bone marrow 13 healthy individuals subsequently expanded...
The standard flow cytometry method for viability testing using 7-aminoactinomycin D (7-AAD) determines cells in necrosis and late apoptosis. colony-forming unit (CFU) assay, which evaluates the proliferation ability of HSCs, is also used graft quality assessment despite known deficiencies that make this assay impractical routine clinical settings. aim was to compare effectiveness 7-AAD/annexin V with 7-AAD assessing HSCs autologous allogeneic peripheral blood stem cell (PBSC) products.
Colony-forming unit (CFU) assay is a short-term culture used for the detection of functionally active hematopoietic progenitor cells (HPCs) in vitro setting. This potency enables identification colony-producing HPCs any type stem cell/progenitor product (HSC/P) including bone marrow (BM), cord blood (CB) and mobilized peripheral (MPB). It has been shown that frequency BM, MPB CB cellular products directly correlates with engraftment both neutrophils platelets as well overall survival...
Methods Cardiovascular tissue allografts (CVA) were retrieved from recipients of heart transplants (RHT) and multiorgan donors (MOD) which fulfill donor selection criteria. Processing CVA was performed in CTB clean room (GMP Class B). Initial processing included surgical dissection, measurements, morphology functional assessment collection quality control samples. incubated antibiotic containing medium (vancomycin, lincomycin, polymyxin B sulfate) for 24-48 hours. Then cryopreserved solution...