A5025918640- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Nanoparticle-Based Drug Delivery
- Cell Adhesion Molecules Research
- Advanced Biosensing Techniques and Applications
- Electrospun Nanofibers in Biomedical Applications
- Extracellular vesicles in disease
- Bone Tissue Engineering Materials
- Fibroblast Growth Factor Research
- Protein Interaction Studies and Fluorescence Analysis
- Network Traffic and Congestion Control
- Lipid Membrane Structure and Behavior
- Tribology and Lubrication Engineering
- Advanced Drug Delivery Systems
- Drug Solubulity and Delivery Systems
- Analytical Chemistry and Chromatography
- Graphene and Nanomaterials Applications
- Computational Drug Discovery Methods
- Antibiotics Pharmacokinetics and Efficacy
- RNA Interference and Gene Delivery
- thermodynamics and calorimetric analyses
- Complex Network Analysis Techniques
- biodegradable polymer synthesis and properties
- Dendrimers and Hyperbranched Polymers
- Polymer Surface Interaction Studies
University of Minho
2018-2022
International Iberian Nanotechnology Laboratory
2020
Universidade Federal de Uberlândia
2011
Collagen is one of the most widely used biomaterials, not only due its biocompatibility, biodegradability and weak antigenic potential, but also to role in structure function tissues. Searching for alternative collagen sources, aim this study was extract from skin codfish, previously obtained as a by-product fish industrial plants, characterize it regarding use biomaterial biomedical application, according American Society Testing Materials (ASTM) Guidelines. type I with high degree purity...
Cancer progression is associated with overexpression of various receptors at the cell surface. Among these, CD44 known to recognize and bind specifically hyaluronan (HA) interact less affinity other glycosaminoglycans (GAGs), such as chondroitin sulfate (CS). In this study, we describe a simple method obtain micellar nanoparticles GAG shell (HA or CS) potential drug delivery systems that target cancer cells overexpressing CD44. Alkanethiol was conjugated reducing end respective using highly...
Hyaluronan (HA) synthesis and degradation are altered during carcinogenesis leading to an increased HA content in the tumor microenvironment, which correlates with poor prognosis treatment outcomes. The main receptors, CD44 RHAMM, also overexpressed tumors where they activate anti-apoptotic, proliferative, invasive, migration signaling pathways. Herein, we used a unidirectional gradient investigate high-throughput fashion bi-directional communication between breast cancer cell lines...
Although it has been repeatedly indicated the importance to develop implantable devices and cell culture substrates with tissue-specific rigidity, current commercially available products, in particular substrates, have rigidity values well above most tissues body. Herein, six resorbable polyester films were fabricated using compression moulding a thermal presser into tailored stiffness by appropriately selecting ratio of their building up monomers (e.g. lactide, glycolide, trimethylene...
Molecular gradients are common in biosystems and play an essential role physiological pathological processes. During carcinogenesis, for example, hyaluronan (HA) homeostasis is dysregulated by cancer cells the altered synthesis degradation processes result formation of HA within tumor microenvironment. Herein, a platform developed to study biological gradient breast cells. Cells with different aggressiveness expression CD44-the main receptor usually overexpressed cancers, selected this...
We report on the synthesis of hyaluronan (HA) brush-like copolymers and their application as antagonists tumorigenic CD44-HA interactions. HA (4.8 kDa, ca. 24 saccharides) was grafted 2-hydrohyethyl methacrylate (HEMA) by end-on oxime ligation. The obtained were compared with low high molecular weight in terms hydrolysis kinetics presence hyaluronidase (isothermal titration calorimetry) interactions CD44 (surface plasmon resonance). results evidenced that HA-g-HEMA have a much higher...
The literature of network traffic analysis has successfully investigated several sophisticated models to be used in computer forecasting. Although these have shown very good results many controlled studies, the complexity their implementation may an important factor for preventing large adoption real production environment. We advocate that simpler forecasting can also show accuracy - similar complex ones scenarios LAN/MAN traffic, and being less intricate implement deploy practical...
Abstract Background. Exosomes are extracellular vesicles originating from the exfoliation of cellular membrane. They involved in cell-to-cell and cell-to-matrix signaling, exchange bioactive molecules, tumorigenesis metastasis, among others. To mitigate limited understanding exosome transfer phenomena, we developed a simplistic model that mimics exosomes their interactions with cells matrix. The proposed is layer-by-layer (LbL) film built polycationic poly-L-lysine (PLL) glycosaminoglycan...
Medical Applications of Glycomaterials In article number 2101309 by Iva Pashkuleva and co-workers, featured in this issue's Special Section, hyaluronan gradients are developed a two-step procedure: i) diffusional deposition colloidal nanoparticles to obtain gradient gold ii) immobilization end-on-thiolated on gradient. These allow high-throughput screening cells. While CD44 negative cells do not change along the gradient, response positive depend level expression density.