A5067765967- Lipid Membrane Structure and Behavior
- Nanoparticle-Based Drug Delivery
- Surfactants and Colloidal Systems
- Advancements in Transdermal Drug Delivery
- Protein Interaction Studies and Fluorescence Analysis
- RNA Interference and Gene Delivery
- Advanced Drug Delivery Systems
- Drug Transport and Resistance Mechanisms
- Analytical Chemistry and Chromatography
- Antioxidant Activity and Oxidative Stress
- Drug Solubulity and Delivery Systems
- Graphene and Nanomaterials Applications
- Free Radicals and Antioxidants
- Advanced biosensing and bioanalysis techniques
- thermodynamics and calorimetric analyses
- Electrospun Nanofibers in Biomedical Applications
- Computational Drug Discovery Methods
- Dermatology and Skin Diseases
- Nanoplatforms for cancer theranostics
- Antibiotics Pharmacokinetics and Efficacy
- Phytochemicals and Antioxidant Activities
- DNA and Nucleic Acid Chemistry
- Sirtuins and Resveratrol in Medicine
- Nanopore and Nanochannel Transport Studies
- HIV/AIDS oral health manifestations
University of Minho
2014-2024
Ikerbasque
2024
University of Cuenca
2018
Max Planck Institute of Experimental Medicine
2018
Clinical Academic Center of Braga
2016
Universidade do Porto
2003-2013
Rede de Química e Tecnologia
2004-2013
Max Planck Institute of Colloids and Interfaces
2008
Introduction: Resveratrol is a polyphenol found in grapes and red wines. Interest this has increased due to its pharmacological cardio- neuroprotective, chemopreventive, antiaging effects, among others. Nevertheless, pharmacokinetic properties are less favorable, since the compound poor bioavailability, low water solubility, chemically unstable. To overcome these problems, we developed two novel resveratrol nanodelivery systems based on lipid nanoparticles enhance resveratrol's oral...
This work focuses on the interaction of four representative NSAIDs (nimesulide, indomethacin, meloxicam, and piroxicam) with different membrane models (liposomes, monolayers, supported lipid bilayers), at pH values, that mimic conditions normal (pH 7.4) inflamed cells 5.0). All are composed 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) which is a phospholipid most cellular membranes. Several biophysical techniques were employed: Fluorescence steady-state anisotropy to study effects in...
Colorectal cancer (CRC) is a heterogeneous disease with high incidence and mortality worldwide. The efficacy of conventional CRC chemotherapy hampered by poor drug solubility bioavailability suboptimal pharmacokinetic profiles. In this work, camptothecin (CPT), potent anticancer drug, was loaded into an amphiphilic chitosan modified PEG oleic acid, to reduce progression after oral administration. While CPT-loaded micelles presented activity against HCT116, Caco-2 HT29 cell lines in vitro,...
The effect of nonsteroidal anti-inflammatory drugs (NSAIDs) on the phase transition and properties 1,2-dipalmitoylphosphatidylcholine (DPPC) has been investigated in both 2D (monolayers at air/water interface) 3D (multilayers lipid/water dispersions) model systems. membrane models have characterized by means pressure−area isotherms grazing incidence X-ray diffraction (GIXD) measurements. Differential scanning calorimetry (DSC) simultaneous small- wide-angle applied to lipid aqueous...
HIV/AIDS stands as a global burden, and vaginal microbicides constitute promising strategy for topical pre-exposure prophylaxis. Preceding the development of microbicide containing tenofovir disoproxil fumarate (TDF) emtricitabine (FTC), in silico vitro studies were performed to evaluate physicochemical characteristics both drugs, study their biophysical impact lipid model systems. Results from these pre-formulation defined hydrogels adequate vehicles incorporate TDF-loaded liposomes FTC....
Increasing evidence suggests a beneficial role of vitamin D (VitD) supplementation in addressing the widespread VitD deficiency, but currently used VitD3 formulations present low bioavailability and toxicity constrains. Hence, poly(L-lactide-co-glycolide) (PLGA) nanoparticles (NPs), solid-lipid (SLNs) nanostructured lipid carriers (NLCs) were investigated to circumvent these issues. PLGA NPs prepared by emulsification or nanoprecipitation presented 74 200 nm, association efficiency (AE) 68 %...
This work focuses on the application of different biophysical techniques to study interaction tolmetin with membrane mimetic models dimensionality (liposomes, monolayers, and supported lipid bilayers) composed 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), used as a representative phospholipid natural membranes. Several were employed: Fluorescence steady-state anisotropy effects NSAIDs microviscosity thus assess main phase transition DPPC, surface pressure–area isotherms evaluate...
Chronic skin exposure to external hostile agents (e.g., UV radiation, microorganisms, and oxidizing chemicals) may increase oxidative stress, causing damage aging. Because of their well-known skincare protective benefits, quercetin (Q) omega-3 fatty acids (ω3) have attracted the attention dermocosmetic pharmaceutical sectors. However, both bioactives inherent properties that limit efficient delivery. Therefore, nanostructured lipid carriers (NLCs) enriched PFC® hydrogels (HGs) been developed...
Posterior eye disorders, such as age-related macular degeneration, diabetic retinopathy, and glaucoma, have a significant impact on human quality of life are the primary cause retinal diseases among adults. There is pressing need for innovative topical approaches to treat posterior current methods often rely invasive procedures with inherent risks. Limited success was attained in realm ophthalmic delivery through non-invasive means. Additionally, there exists dearth literature that delves...
Cell membranes often constitute the first biological structure encountered by drugs, and binding or interactions of drugs with lipid components membrane may explain part their mechanism activity side effects. The present study provides evidence alterations in structural properties phospholipid bilayers at acidic conditions that can be correlated action nonsteroidal anti-inflammatory (NSAIDs) local effect on gastrointestinal tract lipids, aiming a molecular biophysical approach to interaction...
The present work provides a biophysical characterization of the interaction celecoxib, cyclo-oxigenase-2 selective nonsteroidal anti-inflammatory drug, with membranes using liposomes, constituted by phosphatidylcholines, as membrane model systems. In order to mimic biological conditions, experiments were performed at physiological pH (7.4); an acidic conditions inflamed cells (5.0); and different physical states (gel, ripple, fluid phase). Important information regarding celecoxib-membrane...
Current needs for increased drug delivery carrier efficacy and specificity in cancer necessitate the adoption of intelligent materials that respond to environmental stimuli. Therefore, we developed optimized pH-triggered nanoassemblies exhibit an release doxorubicin (DOX) acidic conditions typical tissues endosomal vesicles (pH 5.5) while exhibiting significantly lower under normal physiological 7.5), indicating potential reduce cytotoxicity healthy cells. The hybrid (polymeric/lipid)...