A5032105943- Pluripotent Stem Cells Research
- Zebrafish Biomedical Research Applications
- Single-cell and spatial transcriptomics
- Biomedical Ethics and Regulation
- Wnt/β-catenin signaling in development and cancer
- Hematopoietic Stem Cell Transplantation
- Cell Image Analysis Techniques
- Cancer-related gene regulation
- Reproductive Biology and Fertility
- Reproductive System and Pregnancy
- Drug-Induced Hepatotoxicity and Protection
- Animal Genetics and Reproduction
- Vitamin C and Antioxidants Research
- Pharmacological Effects of Natural Compounds
- Multiple Myeloma Research and Treatments
- Signaling Pathways in Disease
- Renal and related cancers
- Pancreatic function and diabetes
- Organ and Tissue Transplantation Research
University of Cambridge
2022-2025
Wellcome/MRC Cambridge Stem Cell Institute
2022-2025
Medical Research Council
2022
Despite rapid advances in mapping genetic drivers and gene expression changes hematopoietic stem cells (HSCs), there is a relative paucity of studies at the protein level. Here, we perform deep, multi-omic characterization (epigenome, transcriptome proteome) HSCs carrying loss-of-function mutation Tet2, key driver increased self-renewal blood cancers. Using state-of-the-art, multiplexed, low-input mass spectrometry (MS)-based proteomics, profile wildtype (WT) TET2-deficient (Tet2-/-) show...
Summary During mouse gastrulation, extraembryonic mesoderm (ExEM) contributes to the yolk sac (YS) and allantois, both of which are essential for successful gestation. Although genetic networks coordinating intra-embryonic mesodermal subtype specification well-studied, mechanisms driving ExEM diversification poorly understood. Here, we reveal that embryoid body in vitro differentiation generates two distinct lineages cells matching YS allantois respectively. Combining models with vivo...
How distinct mesodermal lineages - extraembryonic, lateral, intermediate, paraxial and axial are specified from pluripotent epiblast during gastrulation is a longstanding open question. By investigating AXIN, negative regulator of the WNT/β-catenin pathway, we have uncovered new roles for WNT signaling in determination fates. We undertook complementary approaches to dissect role that augmented detailed analysis
ABSTRACT Biomedical research relies heavily on the use of model organisms to gain insight into human health and development. Traditionally, mouse has been favored vertebrate model, due its experimental genetic tractability. Non-rodent embryological studies however highlight that many aspects early development, including egg-cylinder topology embryo method implantation, diverge from other mammals, thus complicating inferences about In this study, we constructed a morphological molecular atlas...
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Abstract STAT3 signalling has been studied extensively in the context of self-renewal and differentiation mouse embryonic stem cells. Zygotic is required for normal postimplantation development. On an outbred genetic background, Stat3 null embryos consistently lagged behind their littermates, beginning with significant reduction epiblast cells at implantation. Remarkably, mutants closely resemble non-affected from previous day all stages examined. We pinpoint this phenotype to loss...
SUMMARY At the onset of murine gastrulation, pluripotent epiblast cells migrate through primitive streak, generating mesodermal and endodermal precursors, while ectoderm arises from remaining epiblast. Together, these germ layers establish body plan, defining major axes initiating organogenesis. Although comprehensive single cell transcriptional atlases dissociated mouse embryos across embryonic stages have provided valuable insights during spatial context for differentiation tissue...