A5012088746- Congenital heart defects research
- Zebrafish Biomedical Research Applications
- Angiogenesis and VEGF in Cancer
- Coronary Artery Anomalies
- Hippo pathway signaling and YAP/TAZ
- Pancreatic function and diabetes
- Lymphatic System and Diseases
- Blood Coagulation and Thrombosis Mechanisms
- Insect and Pesticide Research
- Nitric Oxide and Endothelin Effects
- Cardiomyopathy and Myosin Studies
- Cardiac electrophysiology and arrhythmias
- Developmental Biology and Gene Regulation
- Cancer-related molecular mechanisms research
- Connective tissue disorders research
- Wind Turbine Control Systems
- Sympathectomy and Hyperhidrosis Treatments
- Kruppel-like factors research
- Electron Spin Resonance Studies
- Cell Adhesion Molecules Research
University of Saskatchewan
2022-2025
Max Planck Institute for Heart and Lung Research
2016-2024
German Centre for Cardiovascular Research
2020
Collège de France
2009-2014
Centre National de la Recherche Scientifique
2014
Inserm
2009-2014
Sorbonne Université
2014
Centre Interdisciplinaire de Recherche en Biologie
2014
Significance A heart attack occurs when blood flow to part of the is blocked, and oxygen nutrients are unable reach that tissue, irreversibly damaging cardiac muscle cells. Dead cells replaced by a noncontractile scar affects function. Unlike humans, zebrafish can regenerate their after injury, replacing scarred tissue with new cardiomyocytes. Understanding mechanisms deploy may help us design more efficient therapies for human disease. In this study, we show heart, quickly revascularize...
Formation of the lymphatic system requires coordinated expression several key regulators: vascular endothelial growth factor C (VEGFC), its receptor FLT4, and a transcriptional effector, PROX1. Yet, how these signaling components is regulated remains poorly understood. Here, using combination genetic molecular approaches, we identify transcription hematopoietically expressed homeobox (HHEX) as an upstream regulator VEGFC, PROX1 during angiogenic sprouting formation in vertebrates. By...
During cardiac valve development, the single-layered endocardial sheet at atrioventricular canal (AVC) is remodeled into multilayered immature leaflets. Most of our knowledge about this process comes from examining fixed samples that do not allow a real-time appreciation intricacies formation. Here, we exploit non-invasive in vivo imaging techniques to identify dynamic cell behaviors lead formation We find zebrafish, leaflets consist two sets cells luminal and abluminal side, which refer as...
The transcription factor NFATC1 (nuclear of activated T-cell 1) has been implicated in cardiac valve formation humans and mice, but we know little about the underlying mechanisms. To gain mechanistic understanding at single-cell resolution insights into role this process, used zebrafish model as it offers unique attributes for live imaging facile genetics.To understand Nfatc1 formation.Using atrioventricular valve, focus on interstitial cells (VICs), which confer biomechanical strength to...
Electron paramagnetic resonance (EPR) is an excellent choice for detecting free radicals in biological samples. Biologically relevant are extremely short-lived and cannot be detected directly, emphasizing the need appropriate compound to generate stable adducts that can measured by EPR. Spin trapping with nitrone compounds like 5,5-dimethyl-1-pyrroline N-oxide (DMPO) a method commonly employed radicals. However, due instability of radical adducts, using cell-permeable...
ABSTRACT Visualization of protein dynamics is a crucial step in understanding cellular processes. Chromobodies, fluorescently labelled single-domain antibodies, have emerged as versatile probes for live cell imaging endogenous proteins. However, how these chromobodies behave vivo and accurately they monitor tissue changes remain poorly explored. Here, we generated an endothelial-specific β-catenin chromobody-derived probe analyzed its expression pattern during cardiovascular development...
Dicer is an RNase III enzyme that cleaves double stranded RNA and generates functional interfering RNAs act as important regulators of gene protein expression. plays essential role during mouse development because the deletion dicer leads to embryonic death. In addition, dicer-dependent regulate postnatal angiogenesis. However, not yet fully elucidated vascular development.In order explore roles interference in biology, we developed a new constitutive Cre/loxP-mediated inactivation tie2...
SUMMARY Significant efforts have advanced our understanding of foregut-derived organ development; however, little is known about the molecular mechanisms that underlie formation hepatopancreatic ductal (HPD) system. Here, we report a role for homeodomain transcription factor Hhex in directing HPD progenitor specification zebrafish. Loss function results impaired system formation. We found specifies distinct population progenitors gives rise to cystic duct, common bile and extra-pancreatic...