A5084162340- Prostate Cancer Treatment and Research
- Hormonal and reproductive studies
- Prostate Cancer Diagnosis and Treatment
- Urinary Bladder and Prostate Research
- Endometriosis Research and Treatment
- Estrogen and related hormone effects
- Urological Disorders and Treatments
- Reproductive System and Pregnancy
- Uterine Myomas and Treatments
- RNA Research and Splicing
- Connexins and lens biology
- Toxic Organic Pollutants Impact
- Cell Image Analysis Techniques
- AI in cancer detection
- Pregnancy and preeclampsia studies
- Effects and risks of endocrine disrupting chemicals
- Cancer-related gene regulation
- Bone and Dental Protein Studies
- Sexual Differentiation and Disorders
- Neonatal Respiratory Health Research
- Congenital Diaphragmatic Hernia Studies
- Electrolyte and hormonal disorders
- Molecular Biology Techniques and Applications
- Ion Transport and Channel Regulation
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
University of Wisconsin–Madison
2012-2024
University of Wisconsin Carbone Cancer Center
2012-2019
Madison Group (United States)
2012-2015
University of Rochester Medical Center
2007
University of California, San Francisco
2001-2005
University of Missouri
1998-2002
University of San Francisco
2002
Reproductive Science Center
2001
North Dakota State University
1999
Benign prostatic hyperplasia (BPH) and bladder outlet obstruction (BOO) are common in older men can contribute to lower urinary tract symptoms that significantly impact quality of life. Few existing models BOO BPH use physiological levels hormones associated with disease progression humans a genetically manipulable organism. We present model induced mice testosterone (T) 17β-estradiol (E(2)). Male were surgically implanted slow-releasing sc pellets containing 25 mg T 2.5 E(2) (T+E(2)). After...
It has been postulated that prostatic carcinogenesis is androgen dependent and androgens mediate their effects primarily through epithelial cells; however, definitive proof of hormone action in prostate cancer (PRCA) progression lacking. Here we demonstrate genetic loss function experiments PRCA dependency occurs via stromal receptors (AR) but not AR. Utilizing tissue recombination models carcinogenesis, AR was evaluated by surgical castration or deletion. Loss prevented malignant...
Abstract Tissue recombinants (TRs) composed of mouse urogenital mesenchyme (mUGM) plus an immortalized nontumorigenic human prostatic epithelial cell line (BPH‐1) were grown under the kidney capsule male athymic nude mice different hormonal conditions. The objectives to determine temporal plasma concentrations testosterone (T) and estradiol‐17β (E 2 ) that elicit progression cells in vivo . Second, whether mUGM+BPH‐1 TRs [T+E ]‐treated hosts could progress metastases. Control received no...
Prostate disease incidence, both benign and malignant, directly correlates with age. Men under 40 years of age are rarely diagnosed or malignant prostate disease, while 90% men over the 80 have histological evidence (benign prostatic hyperplasia; BPH). Although rodent models been invaluable in study progression treatment efficacy, effect is often not considered. In examining aged (24-month-old) mice, we observed changes within lower urinary tract that typically associated dysfunction (LUTD)...
To explore the identity and possible function of endometriosis protein-I (ENDO-I), which is an acidic glycoprotein synthesized secreted by endometriotic lesions, partial amino acid sequence cDNA were determined. Partially purified, de novo-synthesized rat glycoproteins separated two-dimensional SDS-PAGE, transferred to polyvinyl difluoride membranes, stained with Coomassie blue. Protein corresponding size pI ENDO-I was cut from membranes analyzed automated Edman degradation. analysis...
An important step in the monthly turnover of endometrial lining during menstrual cycle is cyclical recruitment and activation inflammatory cells. Regulated Upon Activation Normal T Cell Expressed Secreted (RANTES) has been shown to mediate cell chemotaxis. This study investigated effect PPAR-gamma ligands upon transcription translation RANTES human stromal First, expression endogenous was confirmed The promoter searched identify likely PPAR response elements (PPREs), which three putative...
Benign prostatic hyperplasia, prostate cancer, and changes in the ratio of circulating testosterone estradiol often occur concurrently aging men can lead to lower urinary tract (LUT) dysfunction. To explore possibility a fetal basis for development LUT dysfunction adulthood, Tg(CMV-cre);Nkx3-1+/−;Ptenfl/+mice, which are genetically predisposed neoplasia, were exposed utero during lactation 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, 1 μg/kg po) or corn oil vehicle (5 ml/kg) after single...
Male lower urinary tract symptoms (LUTS) occur in more than half of men above 50 years age. LUTS were traditionally attributed to benign prostatic hyperplasia (BPH) and therefore the clinical terminology often uses BPH interchangeably. More recently, also linked fibrogenic inflammatory processes. We tested whether osteopontin (OPN), a proinflammatory profibrotic molecule, is increased symptomatic BPH. prostate epithelial stromal cells secrete OPN response stimuli identified downstream...
Significance Little is known regarding the mechanisms involved in loss of androgen receptor (AR) function development castration resistance. Recently, clinical use new potent AR therapies has led to emergence a lethal subtype castration-resistant prostate cancer (CRPC) lacking protein and signaling, termed ARL/negative CRPC. We discovered that DDX3 (DEAD-box helicase 3 X-linked) key repressor translation—a mechanism leads Inhibition CRPC promotes expression protein/signaling hence...
The peroxisome proliferator-activated receptors (PPARs) α and γ are nuclear that play important roles in inflammatory diseases like ulcerative colitis arthritis. In this study, we examined the possible role of PPARs macrophage attraction into peritoneal cavity patients with endometriosis. We identified PPAR-α -γ messenger RNA by RT-PCR protein immunoblotting lysates macrophages monocytic U937 cells. Using immunocytochemistry, localized within nuclei both cell types. Monocyte chemotactic...
Immunohistochemistry is a commonly used clinical and research lab detection technique for investigating protein expression localization within tissues. Many semi-quantitative systems have been developed scoring using immunohistochemistry, but inherent subjectivity limits reproducibility accuracy of results. Furthermore, the investigation spatially overlapping biomarkers such as nuclear transcription factors difficult with current immunohistochemistry techniques. We optimized system...
The objectives of this study were to determine the effects ractopamine HCl (RAC) stereoisomers (RR, RS, SR, and SS) on performance, carcass composition, nitrogen retention in growing female rats. Forty-eight rats (eight rats/treatment) treated with 0 or 320 microg/d RAC 80 RR, SS ractopamine. Rats had free access feed water before during experiment. Ractopamine delivered via i.p. implanted osmotic pumps for 14 d, then slaughtered. Control fitted containing saline. increased (P < .05) intake...
Benign prostatic hyperplasia (BPH) is an age-related disease that affects millions of aging males globally. While the pathogenesis BPH remains incompletely understood, emerging evidence suggests a pivotal role for androgen receptor (AR) in mediating prostate growth and function. Understanding AR signaling alteration may inform novel treatments. Here, we analyzed protein expressions AR, NKX3.1, Ki-67 young (2 months) aged (24 mice. We also examined potential mechanism expression. Compared to...
Abstract Background Prostate cancer (PRCA) is an androgen‐driven disease, where androgens act through the androgen receptor (AR) to induce proliferation and survival of tumor cells. Recently, AR splice variant 7 (ARv7) has been implicated in advanced stages PRCA clinical recurrence. With widespread use AR‐targeted therapies, there a rising interest expression full‐length ARv7 progression how these receptors, both independently together, contribute adverse clinicopathologic outcomes. Methods...
Immunohistochemistry is a commonly used clinical and research lab detection technique for investigating protein expression localization within tissues. Many semi-quantitative systems have been developed scoring using immunohistochemistry, but inherent subjectivity limits reproducibility accuracy of results. Furthermore, the investigation spatially overlapping biomarkers such as nuclear transcription factors difficult with current immunohistochemistry techniques. We optimized system...