A5075616182- Asphalt Pavement Performance Evaluation
- Geotechnical Engineering and Underground Structures
- Infrastructure Maintenance and Monitoring
- Reproductive tract infections research
- Mechanical Behavior of Composites
- Elasticity and Material Modeling
- Rheology and Fluid Dynamics Studies
- Coronary Interventions and Diagnostics
- Structural Response to Dynamic Loads
- Amoebic Infections and Treatments
- Parasitic Infections and Diagnostics
- RNA modifications and cancer
- Tree Root and Stability Studies
- Immune Response and Inflammation
- Material Properties and Processing
- Extracellular vesicles in disease
- Cardiovascular Health and Disease Prevention
- Polysaccharides Composition and Applications
- Material Properties and Failure Mechanisms
- Plant and Fungal Interactions Research
- Reproductive System and Pregnancy
- Plant Surface Properties and Treatments
- Musculoskeletal synovial abnormalities and treatments
- Mechanical Engineering and Vibrations Research
- Wood Treatment and Properties
Stanford University
2020-2025
New York Hospital Queens
2018-2019
Columbia University
2018
NewYork–Presbyterian Hospital
2018
UCSF Benioff Children's Hospital
2016-2017
Max Planck Institute for Infection Biology
2006-2011
Charité - Universitätsmedizin Berlin
2011
Max Planck Society
2008
Pennsylvania State University
1983-2003
Chlamydia are obligate intracellular bacteria that cause variety of human diseases. Host cells infected with protected against many different apoptotic stimuli. The induction apoptosis resistance is thought to be an important immune escape mechanism allowing replicate inside the host cell. Infection C. trachomatis activates Raf/MEK/ERK pathway and PI3K/AKT pathway. Here we show inhibition these two pathways by chemical inhibitors sensitized granzyme B-mediated cell death. leads...
Host cells infected with obligate intracellular bacteria Chlamydia trachomatis are profoundly resistant to diverse apoptotic stimuli. The molecular mechanisms underlying the block in signaling of is not well understood. Here we investigated mechanism by which apoptosis induced via tumor necrosis factor (TNF) receptor prevented epithelial cells. Infection C. leads up-regulation cellular inhibitor (cIAP)-2, and interfering cIAP-2 sensitized for TNF-induced apoptosis. Interestingly, besides...
Extracellular vesicles (EVs) secreted by eukaryotic and prokaryotic cells to transport lipids, proteins, nucleic acids the external environment have important roles in cell-cell communication through cargo transfer. We identified characterized EVs from Entamoeba histolytica, a protozoan parasite human pathogen. Conditioned medium amebic parasites contained particles consistent with expected size morphology of EVs. Mass spectrometry was used characterize EV proteome showed that it enriched...
Chlamydiae are obligate intracellular Gram-negative bacteria that cause widespread diseases in humans. Due to the intimate association between bacterium and host, Chlamydia evolved various strategies protect their host cell against death-inducing stimuli, allowing complete its development cycle. An RNA interference (RNAi)-based screen was used identify factors required for apoptosis resistance of human epithelial cells infected with trachomatis serovar L2. Among 32 validated hits,...
In the present study, we report for first time presence of tRNA-derived fragments in Entamoeba . were identified by bioinformatics analyses small-RNA sequencing data sets from parasites and also confirmed experimentally. We found that tRNA halves accumulated exposed to environmental stress or during developmental process encystation.
Abstract The obligate-intracellular pathogen Chlamydia trachomatis ( Ct) has undergone considerable genome reduction with consequent dependence on host biosynthetic pathways, metabolites and enzymes. Long-chain acyl-CoA synthetases (ACSLs) are key host-cell enzymes that convert fatty acids (FA) into for use in metabolic pathways. Here, we show the complete ACSL family [ACSL1 ACSL3–6] translocates Ct membrane-bound vacuole, termed inclusion remains associated membranes of metabolically active...
Chlamydia trachomatis (Ct) is a Gram-negative obligate intracellular pathogen of humans that causes significant morbidity from sexually transmitted and ocular diseases globally. Ct acquires host fatty acids (FA) to meet the metabolic growth requirements organism. Lipid droplets (LDs) are storehouses FAs in cells have been proposed be source for parasitophorous vacuole, termed inclusion, which replicates. Previously, devoid LDs were shown produce reduced infectious progeny at 24 hr...
The incremental creep and recovery theory was used to develop a constitutive relationship for apple cortex without assuming the nature characteristics of material. Successive loading unloading cycles increasing duration were applied on cylindrically shaped specimens cortex. elastic strain component linear with stress. viscoelastic permanent components time dependent nonlinear functions Three‐step tests stress‐strain at various deformation rates also conducted Prediction strains under these...
Recent studies have shown that extracellular vesicles (EVs) secreted by various parasites are capable of modulating the host’s innate immune responses, such as altering macrophage (M ϕ ) phenotypes and functions. Studies M promote early host responses to amoebic infection releasing proinflammatory cytokines crucial combating amoebiasis. Here, we reporting for first time effect EVs released Entamoeba histolytica ( Eh EVs) on human THP‐1 differentiated (THP‐1 ). We show internalized which...
Abstract The paper reviews the various classical failure theories essentially developed for metals and then seeks to show that same with slight modifications could be used describe of polymeric materials. These consist in consideration effect rate loading on behavior. In addition, reports experimental results two materials under uniaxial tension several biaxial stress fields corresponding pure internal pressure, pressure combined torque loadings cobined loadings. behavior has been studied...
Finite element models of diseased arteries subject to balloon dilation are used study the stresses in arterial wall and plaque at peak pressures. These incorporate large strain, simulate contact between inner artery, constituent materials modeled as nonlinear elastic. In these analyses, pressures range from 300 450 kPa. The stress artery is recorded under different stenosis levels dilated using balloons possible causes restenosis rupture discussed.
Abstract This article describes the experimental investigations on fracture of a polymeric material under various uniaxial and biaxial stress fields, at controlled rates loading. The fields have been produced by subjecting tubular specimens to pure internal pressure, pressure combined with tension loading, torsion loadings. arrangements developed for conducting above tests described. Examination fractured specimen subjected indicates that surface is mostly along plane maximum normal stress....
Long-distance intracellular transport of organelles, mRNA, and proteins ("cargo") occurs along the microtubule cytoskeleton by action kinesin dynein motor proteins, but vast network factors involved in regulating cargo are still unknown. We capitalize on Drosophila melanogaster S2 model cell system to monitor lysosome bundles, which require enzymatically active kinesin-1 protein for their formation. use an automated tracking program a naive Bayesian classifier multivariate motility data...