A5059314111- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Glycosylation and Glycoproteins Research
- Advanced Electron Microscopy Techniques and Applications
- Polymer Surface Interaction Studies
- RNA and protein synthesis mechanisms
- Drug Transport and Resistance Mechanisms
- Protein Interaction Studies and Fluorescence Analysis
- RNA modifications and cancer
University of Pavol Jozef Šafárik
2020-2024
Bioscience (Slovakia)
2024
Institute of Physics of the Slovak Academy of Sciences
2022
X-ray crystallography is an established tool to probe the structure of macromolecules with atomic resolution. Compared alternative techniques such as single-particle cryo-electron microscopy and micro-electron diffraction, uniquely suited room-temperature studies for obtaining a detailed picture subjected external electric field (EEF). The impact EEF on proteins has been extensively explored through single-crystal crystallography, which works well larger high-quality protein crystals. This...
Thermophilic proteins and enzymes are attractive for use in industrial applications due to their resistance against heat denaturants. Here, we report on a thermophilic protein that is stable at high temperatures (Ttrs, hot 67 °C) but undergoes significant unfolding room temperature cold denaturation. Little known about the denaturation of proteins, although it can significantly limit applications. We investigated multidomain translation initiation factor 2 (IF2) from Thermus thermophilus....
Protein aggregation is one of the most critical processes affecting protein solubility in various contexts-from therapeutics formulation to diseases. In general, time-dependent changes are complex kinetically driven that often involve a triggering event consists unfolding/misfolding followed by assembling aggregation-competent species. this study, we have examined relation between stability and recombinant human antibody light chain, hLC, which was found form renal tubular casts multiple...