Prevention of food spoilage and poisoning pathogens is usually achieved by use chemical preservatives which have negative impacts including: human health hazards the applications, residues in & feed chains acquisition microbial resistance to used chemicals. Because such concerns, necessity find a potentially effective, healthy safer natural alternative increased. Within these texts, Plant extracts been control diseases preserve foodstuff. Antimicrobial activity five plant were investigated...

Abstract Secondary plant metabolites remain one of the key sources therapeutic agents despite development new approaches for discovery medicinal drugs. In current study, chemical analysis, and biological activities Kei apple ( Dovyalis caffra ) methanolic extract were evaluated. Chemical analysis was performed using HPLC GC–MS. Antiviral anticancer effect assessed crystal violet technique activity against human liver cells (HepG2), respectively. Antibacterial tested with disc diffusion...

Multiple biological functions of Mentha pulegium extract were evaluated in the current work. Phytochemical components M. detected by Gas Chromatography-Mass Spectrometry (GC-MS) and High-performance liquid chromatography (HPLC). Moreover, was estimated for antioxidant potential 2,2-Diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical scavenging, antimicrobial activity well diffusion, anticoagulant via prothrombin time (PT) activated partial thromboplastin (APTT). GC-MS analysis compounds...

Abstract The use of eco-friendly methods for the synthesis nanoparticles and its nano-composite has become a public demand nowadays to reduce risks chemical methods. In current study, green Cu-doped ZnO based polymers nan-ocomposite was performed. Various instrumental analysis including UV–vis, ATR-FTIR spectroscopy, XRD, SEM coupled with energy dispersive X-ray analysis, TEM Thermal gravimetric were used characterize nano-composite. Highly antibacterial activity synthesized recorded against...

Abstract Of 40 ready-to-eat salads, 3 (7.5%) were positive for Clostridium difficile by PCR. Two isolates PCR ribotype 017 (toxin A–, B+), and 1 was 001. Isolates susceptible to vancomycin metronidazole but variably resistant other antimicrobial drugs. Ready-to-eat salads may be potential sources virulent C. difficile.

Abstract Recently, focus has been placed on renewable sources, as they can be provided in large quantities at the lowest possible cost, order to create nanoparticles. One of these sources is Zygnema moss which used present investigation Copper oxide nanoparticles (CuONPs). Several phenols and flavonoids were identified extract sp. via analysis High performance liquid chromatography. These constituents served reducing stabilizing agents for CuONPs. Characterization CuONPs was performed...

In the last decade, urgent need to explore medicinal plants or drug development has increased enormously around world overcome numerous health problems. present investigation, HPLC indicated existence of 18 phenolic and flavonoid compounds in Cupressus sempervirens extract. Hesperetin represents greatest concentration (25,579.57 µg/mL), while other compounds, such as pyro catechol, rutin, gallic acid, chlorogenic naringenin, quercetin, were recognized concentrations 2922.53 µg/mL, 1313.26...

Background:Clostridium difficile has been shown to be a nosocomial infection associated with diarrhoea and pseudomembranous colitis in hospitalized patients especially old patients. In my previous studies, it was the occurrence of C. animals feces vegetables which may act as source humans. The aim study determine prevalence retail raw cow, sheep, goat, meat Jazan, Saudi Arabia. Method: A total 600 samples from goat were collected during June–December 2015, tested for presence difficile....

Silver nanoparticles (AgNPs) have been applied as an antifungal agent, which results in AgNPs contamination of agricultural waste that interferes with the lignocellulosic enzymes produced by fungi. Therefore, this study examined production carboxymethylcellulase (CMCase) and manganese-dependent lignin peroxidase (MnPase), using barley straw treated AgNPs. Trichoderma asperellum growth was not inhibited at 25 ppm AgNPs, while negligible inhibition observed 50 Aspergillus terreus Curvularia...

The influence of copper and its nanoparticles was studied relative to growth ultrastructure Aspergillus niger. Laccase production by A. niger using corn cobs as substrate at different concentrations CuSO4 (CuNPs) is reported. Fungus induced 100 ppm CuNPs CuSO4, while 300 ppm, the inhibition 65.6% 86.9%, respectively. sporulation reduced 30.4% 47.6% respectively, compared control (100%). Transmission electron microscopy revealed that treatments encouraged deformed appearance fungus 200...

To determine the prevalence of Helicobacter pylori ureA and clarithromycin resistance gene 23S rRNA genotypes among H. in Saudi Arabia.A total 100 serum fecal samples from 70 patients 30 healthy volunteers, who presented with symptoms suggestive gastritis or peptic ulcer disease, were taken main hospital Southern region Arabia September 2010 C.E. to March 2011 corresponding Shawwal 1431 A.H. Rabi Al-Thani 1432 We cultured for a polymerase chain reaction was carried out check presence absence...

Fungicides can have a good result against phytopathogens, but alternatives, such as biological control agents, also been found to be effective and ecofriendly. In this experiment, Alternaria solani was isolated from rotten tomato fruit. The culture filtrates of Trichoderma harzianum gradually inhibited the radial growth A. at higher concentrations, not completely until high filtrate percentage 75% reached (75% by volume). microscopic study revealed that T. its spores changed size, number,...

Saccharomyces cerevisiae is one of the most promising unicellular fungi on account its vital applications in biotechnology as well bioethanol production. Improvement ethanol production via very high-gravity (VHG) fermentation (fermentation at high sugar levels) was successfully developed using ethidium bromide (EtB) mutagenesis S. cerevisiae. This study found two mutants (EtB20a and EtB20b) with varied capacity for EtB, depending random amplified polymorphic DNA analysis. Mutant EtB20b...

Biodegradation is a cost-effective and eco-friendly treatment for oil-contaminated materials using microorganisms. Bacteria fungi can degrade petroleum by it as an energy source this may provide enormous scope to remediate soils contaminated with oil. This study aimed assess the biodegradation of hydrocarbons certain Cladosporium species.By traditional spectroscopic assessment analysis, qualitative screening was carried out spores isolated from air cultured on mineral salt medium...

Abstract In the present study, synthesis and characterisation of titanium dioxide (TiO 2 ) nanoparticles concurrently doped with fluorine (F) manganese (Mn) are studied for improved photocatalytic degradation bacterial inhibition. The absorption spectrum observed at 400 nm is blue shifted to 398 in co-doped TiO , which confirmed increase incorporation F Mn. Transmission electron microscopic images XRD results confirm that size decreased increasing concentration co-dopants. Co-doping Mn by...