A5025146279- Zebrafish Biomedical Research Applications
- Adenosine and Purinergic Signaling
- Receptor Mechanisms and Signaling
- Plant Genetic and Mutation Studies
- Physiological and biochemical adaptations
- Environmental Toxicology and Ecotoxicology
- Advanced Nanomaterials in Catalysis
- Fish Ecology and Management Studies
- Plant Pathogens and Resistance
- Plant tissue culture and regeneration
- Neuroscience of respiration and sleep
- Luminescence Properties of Advanced Materials
- Pharmaceutical and Antibiotic Environmental Impacts
- Carbon and Quantum Dots Applications
- GABA and Rice Research
- Lipid Membrane Structure and Behavior
- Neuroinflammation and Neurodegeneration Mechanisms
- Nanoplatforms for cancer theranostics
- Pharmacological Receptor Mechanisms and Effects
- Carcinogens and Genotoxicity Assessment
- Bacterial biofilms and quorum sensing
- Reproductive biology and impacts on aquatic species
- Leaf Properties and Growth Measurement
- Trace Elements in Health
- Neurotransmitter Receptor Influence on Behavior
Dr. Hari Singh Gour University
2016-2025
Bhailal Amin General Hospital
2022
Sri Venkateswara University
2016
Max Planck Society
2007-2013
Wolfson Foundation
2012-2013
University College London
2012-2013
Max Planck Institute of Biophysics
2007-2012
All India Institute of Medical Sciences
2001
Rashtrasant Tukadoji Maharaj Nagpur University
1982-1986
P2X receptors are ATP-gated nonselective cation channels. Functional assembled as homotrimers or heterotrimers of seven cloned subunits. Each subunit contains two transmembrane domains linked by a large extracellular loop that is required for agonist binding. So far, there no direct evidence indicating whether the binding site formed within one at interface neighboring Here we used disulfide cross-linking approach to identify pairs residues in close proximity ATP 1 homotrimer. Eight amino...
P2X receptors (P2XRs) are ligand-gated ion channels activated by extracellular ATP. Although the crystal structure of zebrafish P2X4R has been solved, exact mode ATP binding and conformational changes governing channel opening desensitization remain unknown. Here, we used voltage clamp fluorometry to investigate movements in cysteine-rich head domain rat P2X1R (A118-I125) that projects over proposed site. On substitution with cysteine residues, six these residues (N120–I125) were...
Genetically-encoded biosensors are powerful tools for understanding cellular signal transduction mechanisms. In aiming to investigate cGMP signaling in neurones using the EGFP-based fluorescent biosensor, FlincG (fluorescent indicator cGMP), we encountered weak or non-existent fluorescence after attempted transfection with plasmid DNA, even HEK293T cells. Adenoviral infection of cells FlincG, however, had previously proved successful. Both constructs were found harbor a mutation EGFP domain...
Pure Eu3+ ion doped BaF2 nanoparticles with tunable properties or property combinations are accessible via an ionic liquid-assisted solvothermal method. Structural parameters such as cell parameters, lattice strain, and especially morphology judiciously tuned calcination temperatures. For example, tensile strain is observed in samples calcined up to 400 °C; however, compressive appears at 600 °C beyond. Larger surface area the sample observation of layer structure higher calcinations...
Rhizobia are vital for nitrogen input, fertility of soil and legume plant growth. Knowledge on rhizobial diversity from arid semiarid areas is important dry land agriculture in the context climatic change economic utilization. This study provides morphological, biochemical, stress tolerance growth promoting characteristics fifteen isolates nodules same number wild legumes one isolate cultivated Arachis hypogea semi-arid region, Tirupati. The bacterial were confirmed as rhizobia based colony...
For the assessment of cardiac function, heartbeat represents one key parameter. Current methods measurements in zebrafish larvae usually require larval immobilization, fluorescent transgenic strains and a confocal microscope, costly commercial software for analysis, or strong programming skills if is open-source. Here, we present simple yet powerful method analysis using untethered, unlabeled larva ImageJ (open-source software), which does not skills. We named it as ZebraPace Zebrafish...
A 21-year-old male patient was admitted with acute renal failure and intravascular hemolysis following suicidal parenteral copper sulfate poisoning. He developed metabolic acidosis septicemia; treated intensive hemodialysis, blood transfusions antibiotics. After remaining anuric for 4 weeks, his urine output gradually increased. However functions improved only partially. Renal biopsy done 8 weeks after the episode showed chronic tubulo-interstitial nephritis (CIN). This is first reported...
Abstract Inorganic nanohosts doped with lanthanide ions are an indispensable tool for multimodal bioimaging and MRI applications due to their distinctive optical, magnetic, biocompatible properties. Nanocomposites of such nanoprobes functionalization capabilities could also enable use in targeted therapy‐based applications. However, the influence nanocomponents especially proportion a nanocomposite modulating physio‐chemical biocompatibility properties is still elusive. To address this...
Synthesis of high-performance N-doped carbon dots (TNCDs) with an enhanced quantum yield for dual imaging and their in vivo toxicity evaluation a zebrafish model.
In P2X receptors, agonist binding at the interface between neighbouring subunits is efficiently transduced to ion channel gating. However, relationship and gating difficult study because agonists continuously bind unbind. Here, we covalently incorporated in pocket of receptors examined how site occupancy affects ability gate.We used a strategy for tethering their ATP-binding pocket, while simultaneously probing using electrophysiology. The 2',3'-O-(4-benzoylbenzoyl)-ATP (BzATP),...
Zebrafish is a vertebrate animal model. Their maintenance in large number under laboratory conditions daunting task. Commercially available recirculating zebrafish systems are used to efficiently handle the tasks of automatic sediment cleaning from tanks with minimal waste water. Due their compact nature, they also ensure maximal use lab space. However, high costs commercial present limitation researchers limited funds. A cost-effective system major features offered by commercially highly...
Maintenance of large number zebrafish in captive conditions is a daunting task. This can be eased by the use recirculating racks with self-cleaning tanks. Commercially available systems are costly, and compatibility intercompany products has never been investigated. Although various cost-effective designs methods construction custom-made literature, design tanks still not available. In this study, we report method unit, which fitted any tank. We validated investigating sediment cleaning...
Nanoparticles with biomedical applications should be evaluated for their biocompatibility.