A5038920470- Prion Diseases and Protein Misfolding
- Alzheimer's disease research and treatments
- RNA Research and Splicing
- Protein Structure and Dynamics
- Trace Elements in Health
- Neurological diseases and metabolism
- RNA regulation and disease
- Advanced NMR Techniques and Applications
- Amino Acid Enzymes and Metabolism
- Fungal and yeast genetics research
- NMR spectroscopy and applications
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Nuclear Structure and Function
- Parkinson's Disease Mechanisms and Treatments
- Bioinformatics and Genomic Networks
- Biochemical and Molecular Research
- Advanced Neuroimaging Techniques and Applications
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Microbial Metabolic Engineering and Bioproduction
- Microbial metabolism and enzyme function
- Microtubule and mitosis dynamics
- PI3K/AKT/mTOR signaling in cancer
- Digital Transformation in Industry
St Petersburg University
2016-2025
In this work two modes of binding the fluorescent probe thioflavin T to yeast prion protein Sup35p amyloid fibrils were revealed by absorption spectrometry solutions prepared equilibrium microdialysis. These exhibited significant differences in affinity and stoichiometry. Moreover, spectrum molar extinction coefficient dye bound each mode determined. The fluorescence quantum yield was determined via a spectrofluorimetric study same which recorded intensity corrected for primary inner filter...
Genetically encoded voltage indicators (GEVIs) have found wide applications as molecular tools for visualization of changes in cell membrane potential. Among others, several classes archaerhodopsin-3-based GEVIs been developed and proved themselves promising various imaging studies. To expand the application range this type GEVIs, new variants with absorption band maxima shifted toward first biological window enhanced fluorescence signal are required. Here, we integrate computational...
The essential SUP35 gene encodes yeast translation termination factor Sup35/eRF3. N-terminal domain of Sup35 is also responsible for prionization that leads to generation the [PSI+] prion. Previously we isolated different types sup35 mutations (missense and nonsense) demonstrated nonsense (sup35-n) are incompatible with prion, leading lethality sup35-n haploid cells. Here, show missense (sup35-m) within conservative regions C-domain result in cells because weak activity Sup35/eRF3 as a...
Numerous experimental studies have suggested that polypeptide chains of large amyloidogenic regions zig-zag in β-serpentine arrangements. These β-serpentines are stacked axially and form the superpleated β-structure. Despite this progress understanding amyloid folds, determination their 3D structure at atomic level is still a problem due to polymorphism these fibrils incompleteness structural data. Today, way get insight into amyloids combination with bioinformatics.We developed computer...
[PSI+ ] is the prion form of translation termination factor Sup35 (eRF3); strains display nonsense suppression. Another prion-like element, [ISP+ ], linked to antisuppression in a specific background. Transcriptional regulator Sfp1 was shown be responsible for propagation. In this work, we identified SFP1 as multicopy inducer ]-dependent lethality. likely up-regulate transcription genes encoding release factors; however, its overproduction increases Sup35, but not Sup45 protein level. Using...
Amyloids are fibrillar protein aggregates with a cross-β structure. More than two hundred different proteins amyloid or amyloid-like properties already known. Functional amyloids conservative amyloidogenic regions were found in organisms. Protein aggregation appears to be beneficial for the organism these cases. Therefore, this property might orthologous proteins. The of CPEB suggested play an important role long-term memory formation Aplysia californica, Drosophila melanogaster, and Mus...
The NOS1AP gene encodes a cytosolic protein that binds to the signaling cascade component neuronal nitric oxide synthase (nNOS). It is associated with many different disorders, such as schizophrenia, post-traumatic stress disorder, autism, cardiovascular and breast cancer. (also known CAPON) mediates within complex which includes NMDA receptor, PSD-95, nNOS. This adapter involved in (NO) synthesis regulation via its association nNOS (NOS1). Our bioinformatics analysis revealed an...
Amyloids are protein fibrils with a characteristic spatial structure. were long perceived as the pathogens involved in set of lethal diseases humans and animals. In recent decades, it has become clear that amyloids represent quaternary structure is not only pathological but also functionally important widely used by different organisms, ranging from archaea to animals, implement diverse biological functions. The greatest variety found prokaryotes, where they control formation biofilms cell...
ABSTRACT Semi-denaturing detergent agarose gel electrophoresis (SDD-AGE) was proposed by Vitaly V. Kushnirov in the Michael D. Ter-Avanesyan's laboratory as a method to compare sizes of amyloid aggregates. Currently, this is widely used for investigation, but mostly qualitative approach. In work, we assessed possibilities and limitations quantitative analysis aggregate size distribution using SDD-AGE results. For purpose, aggregates two well-characterized yeast amyloid-forming proteins,...
A number of [PSI +]-no-more (PNM) mutations, eliminating +] prion, were previously described in SUP35. In this study, we designed and analyzed a new PNM mutation based on the parallel in-register β-structure Sup35 prion fibrils suggested by known experimental data. such an arrangement, substitution non-charged residues charged ones may destabilize fibril structure. We introduced Q33K/A34K amino acid substitutions into protein, corresponding allele was called sup35-M0. The mutagenized chosen...
Modern biology requires modern genetic concepts equally valid for all discovered mechanisms of inheritance, either "canonical" (mediated by DNA sequences) or epigenetic. Applying basic terms such as "gene" and "allele" to protein hereditary factors is one the necessary steps toward these concepts. The idea that different variants same prion can be considered alleles has been previously proposed Chernoff Tuite. In this paper, notion allele further developed. We propose any a bimodular system...
Amyloids are fibrillar protein aggregates with a cross-β structure and unusual features, including high resistance to detergent or protease treatment. More than two hundred different proteins amyloid amyloid-like properties already known. Several examples of nucleoporins (e.g., yeast Nup49, Nup100, Nup116, human NUP153) supposed form fibrils. In this study, we demonstrated an ability the NUP58 nucleoporin in vivo vitro. Moreover, found forms aggregates: oligomers polymers stabilized by...
The review discusses the role that proteins interacting with translation termination factors eRF1 and eRF3 play in control of protein synthesis prionization. These interact not only each other, but also many other involved controlling efficiency termination, associate cell processes. is directly related to re-initiation ribosome recycling, mRNA stability quality control. This connection ensured by interaction participating various metabolic processes, such as transport from nucleus into...