ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Search for a Taxol-Producing Microorganism Among the Endophytic Fungi of Pacific Yew, Taxus brevifoliaAndrea Stierle, Gary Strobel, Donald Paul Grothaus, and BignamiCite this: J. Nat. Prod. 1995, 58, 9, 1315–1324Publication Date (Print):September 1, 1995Publication History Published online1 July 2004Published inissue 1 September 1995https://pubs.acs.org/doi/10.1021/np50123a002https://doi.org/10.1021/np50123a002research-articleACS...

Berkeley Pit Lake is an abandoned open-pit copper mine filled with 30 billion gallons of acidic, metal-contaminated water. This harsh environment proving to be a source unusual microorganisms that produce novel bioactive metabolites. Bioassay-guided fractionation using signal transduction enzyme assays led the isolation spiroketal, berkelic acid 1, and known gamma-pyrone, spiciferone A 4. Berkelic has shown selective, nanomolar activity against OVCAR-3, ovarian cancer cell line in National...

A carefully timed coculture fermentation of Penicillium fuscum and P. camembertii/clavigerum yielded eight new 16-membered-ring macrolides, berkeleylactones A–H (1, 4, 6–9, 12, 13), as well the known antibiotic macrolide A26771B (5), patulin, citrinin. There was no evidence production or (5) by either fungus when grown axenic cultures. The structures were deduced from analyses spectral data, absolute configurations compounds 1 9 determined single-crystal X-ray crystallography....

This is a review of bioactive secondary metabolites isolated from conifer-associated endophytic fungi 1990-2014. includes compounds with antimicrobial, anti-inflammatory, anti-proliferative and cytotoxic activity towards human cancer cell lines, against either plant pathogens or insect pests. Compounds that were originally reported without associated included if other studies ascribed to these compounds. not they exclusively phytotoxic active extracts but determined be the component extract.

The Berkeley Pit, an acid mine waste lake, is a source of extremophilic microorganisms that produce interesting bioactive compounds. We have previously reported the isolation berkeleydione (1), berkeleytrione (2), berkeleyacetals, and berkeleyamides from Pit Lake fungus Penicillium rubrum. In this paper we report characterization berkeleyones A-C (4, 5, 7) as well described preaustinoid A (3) A1(6) same fungus. These compounds were evaluated inhibitors signaling enzyme caspase-1 potential...

We previously reported several novel bioactive hybrid polyketide-terpenoid metabolites from a deep water Penicillium rubrum isolated Berkeley Pit Lake, Butte, Montana. In this paper we report the structures of four new amides, berkeleyamides A-D (1, 4, 5, 7), extracts fungus. The these compounds were deduced by analysis NMR data, chemical derivatization, and comparison their spectroscopic data to those known compounds.

Allergic asthma is a chronic airway inflammatory disease involving the complementary actions of innate and adaptive immune responses. Endogenously generated cannabinoids acting via CB2 receptors play important roles in both homeostatic processes. However, contribution CB2-acting eicosanoids to events preceding sensitization common house dust mite (HDM) allergen remains be elucidated. We investigated role activation during allergen-induced pulmonary inflammation natural killer (NK) cell...

Two novel hybrid polyketide-terpenoid metabolites were isolated from a Penicillium sp. growing in the Berkeley Pit Lake of Butte, Montana. Their structures deduced by spectroscopic analysis and confirmed single-crystal X-ray on berkeleydione (1). Both compounds inhibited matrix metalloproteinase-3 caspase-1, showed activity toward non-small-cell lung cancer NCI's human cell line antitumor screen.

Berkeley Pit Lake is a 1500 ft deep abandoned open-pit copper mine filled with over 1140 billion liters of acidic, metal-sulfate-contaminated water. This harsh environment proving to be source unusual microorganisms that produce novel bioactive compounds. We recently reported the structures berkeleydione (1) and berkeleytrione (2), two hybrid polyketide-terpenoid metabolites isolated from water Penicillium sp. growing in Lake. In this paper we report three new compounds, berkeleyacetals A–C...

Berkeley Pit Lake, Butte, Montana, is a 540 m deep abandoned open-pit copper mine filled with over 140 billion liters of acidic, metal-sulfate-contaminated water. This harsh environment has yielded several microorganisms that produce interesting biologically active compounds. Several polyketide metabolites including the new berkazaphilones A (1) and B (2) octadienoic acid derivatives berkedienoic (13) berkedienolactone (15), as well previously reported azaphilone 4, vermistatin (6),...

Two new drimane sesquiterpene lactones and one tricarboxylic acid derivative were isolated from the Berkeley Pit extremophilic fungus Penicillium solitum. The structures of these compounds deduced by spectroscopic analysis. Berkedrimanes A B inhibited signal transduction enzymes caspase-1 caspase-3 mitigated production interleukin 1-β in induced THP-1 (pro-monocytic leukemia cell line) assay.

An extremophilic fungus identified as a Pleurostomophora sp. was isolated from the Berkeley Pit, an acid mine waste lake. When grown in liquid culture, produced berkchaetoazaphilones A–C (1, 2, and 5), red pigment berkchaetorubramine (6), known compound 4-(hydroxymethyl)quinoline. These compounds were evaluated inhibitors of matrix metalloproteinase-3, caspase-1, proinflammatory cytokine production induced THP-1 cells. Berkchaetoazaphilone B (2) inhibited IL-1β, TNFα, IL-6 inflammasome assay...

Aspergillus parasiticus, a fungal isolate from the bark of redwood tree (Sequoia sempervirens), has been shown to produce antitumor metabolites sequoiatones A and B more recently C−F. We have also isolated another series compounds with new carbon skeleton, sequoiamonascins. The structures sequoiamonascins A−D were deduced by interpretation their spectral data that some reaction products. brine shrimp lethality-guided fractionation submitted NCI for anticancer evaluation.

Investigation of the bioactive compounds from a Penicillium sp. isolated inner bark Pacific yew, Taxus brevifolia, led to isolation known furanone 1, and series phomopsolides. The phomopsolide fractions contained phomopsolides A B, which have previously been described, three new structures were deduced by comparison their NMR spectra those compounds.

Sequoiatones A and B were isolated from the fungus Aspergillus parasiticus, an endophytic of coast redwood, Sequoia sempervirens. The compounds methanol extract mycelial mat when grown in liquid culture for 21 days. because their brine shrimp lethality, which served as excellent guide chromatographic purification. Full details isolation characterization sequoiatones are provided herein, along with test results NCI human tumor 60 cell-line screen.