Inner nuclear membrane proteins interact with chromosomes in the nucleus and are important for chromosome activity. Lem2 Man1 conserved members of LEM-domain protein family. Mutations associated laminopathy, but their cellular functions remain unclear. Here, we report that maintains genome stability fission yeast Schizosaccharomyces pombe. S. pombe cells disrupted lem2(+) gene (lem2∆) showed slow growth increased rate minichromosome loss. These phenotypes were prominent rich culture medium,...

In budding yeast, Set2 catalyzes di- and trimethylation of H3K36 (H3K36me2 H3K36me3) via an interaction between its Set2-Rpb1 (SRI) domain C-terminal repeats RNA polymerase II (Pol2) phosphorylated at Ser2 Ser5 (CTD-S2,5-P). H3K36me2 is sufficient for recruitment the Rpd3S histone deacetylase complex to repress cryptic transcription from transcribed regions. fission also responsible methylation, which represses a subset RNAs including heterochromatic subtelomeric RNAs, least in part Clr6 II,...

Xenobiotic drugs induce Pleiotropic Drug Resistance (PDR) genes via the orthologous Pdr1/Pdr3 transcription activators. We previously identified Mediator co-activator complex as a key target of Pdr1 orthologs and demonstrated that interacts directly with Gal11/Med15 subunit complex. Based on an interaction between FACT complex, we show strains spt16 or pob3 mutations are sensitive to xenobiotic display diminished PDR gene induction. Although acts during activation some by assisting in...

ABSTRACT Heterochromatin protein 1 (HP1) is a highly conserved, canonical factor involved in heterochromatin formation. HP1 has been shown to interact with proteins other than silencing factors and effectors. In fission yeast, the loss of homolog Swi6 disrupts structure affects mating type switching at mat locus, where exists; however, cell growth unaffected. this study, we focused on dimerization domain, which provides binding surface for various interactors. We isolated distinctive...

Abstract Chromatin-modifying factors regulate both transcription and DNA replication. The yFACT chromatin-reorganizing complex is involved in processes, the sensitivity of some mutants to replication inhibitor hydroxyurea (HU) one indication a role. This HU can be suppressed by disruptions SET2 or CHD1 genes, encoding histone H3(K36) methyltransferase chromatin remodeling factor, respectively. additive effect set2 chd1 mutations suppressing suggests that these two function separate pathways....

H3K9 methylation (H3K9me) is a conserved marker of heterochromatin, transcriptionally silent chromatin structure. Knowledge the mechanisms for regulating heterochromatin distribution limited. The fission yeast JmjC domain-containing protein Epe1 localizes to mainly through its interaction with Swi6, homologue 1 (HP1), and directs JmjC-mediated H3K9me demethylation in vivo. Here, we found that loss epe1 (epe1Δ) induced red-white variegated phenotype red-pigment accumulation background...

FACT (facilitate chromatin transcription) is involved in heterochromatic silencing, but its mechanisms and function remain unclear. We reveal that the Spt16 recruitment mechanism operates two distinct ways heterochromatin. First, Pob3 mediates onto heterochromatin through dimerization tandem PH domains. Without Pob3, partially reduced, exhibiting a silencing defect impaired H2A/H2B organization. Second, protein 1 (HP1)/Swi6 by physical interaction of Swi6 chromo-shadow domain (CSD)...

Rpd3(L) and Rpd3(S) are distinct multisubunit complexes containing the Rpd3 histone deacetylase. Disruption of GCN5 acetyltransferase gene shows a strong synthetic phenotype when combined with either an sds3 mutation affecting only complex or rco1 Rpd3(S). However, these growth defects not seen in gcn5 triple mutant, suggesting that balance between is critical cells lacking Gcn5. Different genetic interactions mutations FACT chromatin reorganizing complex. An has defect mutants, while eaf3...

The yeast HO gene is tightly regulated, with multiple activators and coactivators needed to overcome repressive chromatin structures that form over this promoter. Coactivator binding strongly interdependent, as loss of one factor sharply reduces recruitment other factors. Rpd3(L) histone deacetylase recruited at two distinct times during the cell cycle, first by Ash1 URS1 region promoter then SBF/Whi5/Stb1 URS2. SBF itself localized only a subset its potential sites in URS2, localization...

Cells devote considerable resources to nutrient homeostasis, involving surveillance, acquisition, and storage at physiologically relevant concentrations. Many Saccharomyces cerevisiae transcripts coding for proteins with uptake functions exhibit peak periodic accumulation during M phase, indicating that an important aspect of homeostasis involves transcriptional regulation. Inorganic phosphate is a central macronutrient we have previously shown oscillates inversely mitotic activation PHO5....

In fission yeast, Schizosaccharomyces pombe, constitutive heterochromatin defined by methylation of histone H3 lysine 9 (H3K9me) and its binding protein Swi6/HP1 localizes at the telomere, centromere, mating-type loci. These loci contain DNA sequences called dg dh, RNA interference (RNAi)-dependent system establishes maintains dg/dh. Bi-directional transcription dg/dh induced polymerase II is critical in RNAi-dependent formation because transcribed RNAs provide substrates for siRNA synthesis...

Abstract Chromatin condensation state is the key for retrieving genetic information. High‐mobility group protein (HMG) proteins exhibit DNA‐binding and bending activities, playing an important role in regulation of chromatin structure. We have shown that nucleosomes tightly packaged into heterochromatin undergo considerable dynamic histone H2A‐H2B maintenance via direct interaction between HP1/Swi6 facilitate transcription (FACT), which composed Spt16/Pob3 heterodimer Nhp6. In this study, we...

TFIID, a multiprotein complex composed of TATA element-binding protein (TBP) and 14 TBP-associated factors (TAFs), can directly recognize core promoter elements mediate transcriptional activation. The TAF N-terminal domain (TAND) TAF1 may play significant role in these two principal TFIID functions by regulating the access TBP to element. In yeast, TAND consists subdomains, TAND1 (10-37 amino acids (aa)) TAND2 (46-71 aa), which interact with concave convex surfaces TBP, respectively. Here we...

General transcription factor TFIID is comprised of TATA-binding protein (TBP) and TBP-associated factors (TAFs), together playing critical roles in regulation initiation. The TAF N-terminal domain (TAND) yeast TAF1 containing two subdomains, TAND1 (residues 10-37) TAND2 46-71), sufficient to interact with TBP suppress the TATA binding activity TBP. However, detailed structural analysis complex between TAND12 6-71) was hindered by its poor solubility stability solution. Here we report a...

H2A.Z, an evolutionally well-conserved histone H2A variant, is involved in many biological processes. Although the function of H2A.Z euchromatic gene regulation well known, its and deposition mechanism heterochromatin are still unclear. Here, we report that plays multiple roles fission yeast heterochromatin. While a small amount localizes at pericentromeric heterochromatin, loss methylation H3 Lys9 (H3K9me) induces accumulation which dependent on loader, SWR complex. The accumulated...

The general transcription factor TFIID is composed of TATA-binding protein (TBP) and 14 TBP-associated factors (TAFs). mediates the transcriptional activation a subset eukaryotic promoters. N-terminal domain (TAND) TAF1 (Taf1p) inhibits TBP by binding to its concave convex surfaces. This study examines role TAND in regulation tests whether an autonomous regulator TBP. binds regulates function when it fused amino or carboxy terminus Taf1p, Taf5p, Taf11p. However, carboxy-terminal fusion...

Abstract In fission yeast, siRNA generated by RNA interference (RNAi) factors plays critical roles in establishment and maintenance of heterochromatin. To achieve efficient synthesis, RNAi assemble on heterochromatin via association with Swi6, a homologue protein 1 (HP1), heterochromatic noncoding (hncRNA) retained chromatin. addition, spliceosomes formed hncRNA introns recruit to Small nuclear RNAs, components the spliceosome, have trimethylguanosine (TMG) cap that is Tgs1‐dependent...

DNA is stored in the nucleus of a cell folded state; however, only necessary genetic information extracted from required group genes. The key to extracting chromatin ambivalence. Depending on chromosomal region, characterized into low-density "euchromatin" and high-density "heterochromatin", with various factors being involved its regulation. Here, we focus regulation gene expression by yeast FACT complex, which functions both euchromatin heterochromatin....

Transcription factor II D (TFIID), a multiprotein complex consisting of TATA-binding protein (TBP) and 13-14 TBP-associated factors (Tafs), plays central role in transcription regulates nearly all class genes. The N-terminal domain Taf1p (TAND) can be divided into two subdomains, TAND1 TAND2, which bind to the concave convex surfaces TBP, respectively. interaction between TAND TBP is thought regulated by TFIIA, activators and/or DNA during transcriptional activation, as TAND1-bound form...