- Microbial Fuel Cells and Bioremediation
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Bacterial biofilms and quorum sensing
- Corrosion Behavior and Inhibition
- Nanoparticles: synthesis and applications
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Sensors
- Supercapacitor Materials and Fabrication
- Advanced Photocatalysis Techniques
- Metal Extraction and Bioleaching
- Advanced Nanomaterials in Catalysis
- Hydrogen embrittlement and corrosion behaviors in metals
- Microbial Inactivation Methods
- Biosensors and Analytical Detection
- Concrete Corrosion and Durability
- Laser-Ablation Synthesis of Nanoparticles
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Infective Endocarditis Diagnosis and Management
- Conducting polymers and applications
- Electrocatalysts for Energy Conversion
- bioluminescence and chemiluminescence research
- Antimicrobial agents and applications
- Microbial Community Ecology and Physiology
Singapore Centre for Environmental Life Sciences Engineering
2013-2025
Nanyang Technological University
2013-2025
University of Nottingham Ningbo China
2022-2025
Nazarbayev University
2019-2024
Aarhus University
2024
Ningbo University
2023
National University of Singapore
2017-2019
University of Electronic Science and Technology of China
2017
Dublin City University
2009-2016
Biotechnology Institute
2006-2010
Bacteria able to transfer electrons metals are key agents in biogeochemical metal cycling, subsurface bioremediation, and corrosion processes. More recently, these bacteria have gained attention as the of from cell surface conductive materials can be used multiple applications. In this work, we adapted electrochemical techniques probe intact biofilms Shewanella oneidensis MR-1 sp. MR-4 grown by using a poised electrode an electron acceptor. This approach detected redox-active molecules...
ABSTRACT While electrochemical characterization of enzymes immobilized on electrodes has become common, there is still a need for reliable quantitative methods study electron transfer between living cells and conductive surfaces. This work describes growth thin (<20 μm) Geobacter sulfurreducens biofilms polished glassy carbon electrodes, using stirred three-electrode anaerobic bioreactors controlled by potentiostats nondestructive voltammetry techniques viable biofilms. Routine in vivo...
A simple one-pot green chemical method for the biosynthesis of gold nanoparticles (AuNPs) by reducing chloroauric acid (HAuCl4) with protein extract Rhizopus oryzae to produce novel nano-bio-conjugates (AuNBC) is described. AuNBCs, having sizes ranging from 5 65 nm, were synthesized altering HAuCl4–protein ratio. The conjugates characterized spectroscopic, electron microscopic, light scattering and electrophoretic mobility measurements. It was found that aqueous AuNBC suspensions exhibited...
Microbially influenced corrosion (MIC), is acknowledged to be the direct cause of catastrophic failures, with associated damage costs ranging many billions US$ annually. In spite extensive research and numerous publications, fundamental questions relating MIC remain unanswered. The following review provides an overview current stresses lack information related recognition, prediction mitigation. establishes a link between management decisions root causes. A holistic, proactive approach...
Abstract The ability of Geobacter sulfurreducens to utilize electrodes as electron acceptors provides a system for monitoring mechanisms transfer beyond the cell surface. This study examined physiology extracellular during many stages growth, and in response short‐ long‐term changes acceptor potential. When G. was grown on planar potentiostat‐controlled electrodes, magnitude early attachment increased with initial density. However, first cells attach did not demonstrate same rates...
The advanced research and development of silver nanoparticles (AgNPs) is vast due to their incredible applications today. In this work, AgNPs were synthesized using soil derived Pseudomonas putida MVP2. formation on the P. cell membrane its free supernatant was studied. characterized by UV–visible spectroscopy, scanning transmission electron microscopy (STEM), X-ray diffraction (XRD), energy dispersive (EDAX) Fourier transform infrared (FTIR) spectrum analysis. mode action bacteria studied...
Abstract Bacteria able to transfer electrons conductive surfaces are of interest as catalysts in microbial fuel cells, well bioprocessing, bioremediation, and corrosion. New procedures for immobilization Geobacter sulfurreducens on graphite electrodes described that allow routine, repeatable electrochemical analysis cell–electrode interactions. Immediately after immobilizing G. electrodes, electrical current was obtained without addition exogenous electron shuttles or electroactive polymers....
Power generated by microbial fuel cells is computed as a product of current passing through an external resistor and voltage drop across this resistor. If the applied resistance very low, then high instantaneous power cell measured, which not sustainable; cannot deliver that much for long periods time. Since using small electrical resistors leads to erroneous assessment capabilities cells, question arises: what should be used in such measurements? To address question, we have defined...
In recent years, there has been significant progress in the biological synthesis of nanomaterials. However, molecular mechanism gold biomineralization microorganisms industrial relevance remains largely unexplored. Here we describe biosynthesis nanoparticles (AuNPs) fungus Rhizopus oryzae. Reduction AuCl4– [Au(III)] to nanoparticulate Au0 occurs both cell wall and cytoplasmic region R. The average size as-synthesized AuNPs is ∼15 nm. through adsorption, initial reduction Au(I), followed by...
Enterococci are important human commensals and significant opportunistic pathogens. Biofilm-related enterococcal infections, such as endocarditis, urinary tract wound surgical site medical device-associated often become chronic upon the formation of biofilm. The biofilm matrix establishes properties that distinguish this state from free-living bacterial cells increase tolerance to antimicrobial interventions. metabolic versatility enterococci is reflected in diversity complexity environments...
Three-dimensional nickel–iron (3-D/Ni–Fe) nanostructures are exciting candidates for various applications because they produce more reaction-active sites than 1-D and 2-D nanostructured materials exhibit attractive optical, electrical catalytic properties. In this work, freestanding 3-D/Ni–Fe interconnected hierarchical nanosheets, nanospheres, porous nanospheres directly grown on a flexible carbon fiber paper (CFP) substrate by single-step hydrothermal process. Among the nanostructures,...
The optimized ZnMn 2 O 4 nanoparticles were fabricated through an AV-assisted sol–gel method and employed to form crossed g-C 3 N monolayers as innovative solution for the delamination of stacked layers.
This paper describes a study on the use of polypropylene (PP)/layered silicate nanocomposite as packaging film, agricultural and automotive panels. The study's main question was "Are environmental impacts costs throughout life cycle products lower than those manufactured from conventional materials?" (benchmark) materials studied were pure polyethylene glass fiber-reinforced In all three cases, PP resulted in reduction amount material used, while ensuring same functionality. Material...
The biosynthesis of the redox shuttle, phenazines, in Pseudomonas aeruginosa, an ubiquitous microorganism wastewater microflora, is regulated by 2-heptyl-3,4-dihydroxyquinoline (PQS) quorum-sensing system. However, PQS inhibits anaerobic growth P. aeruginosa. We constructed a aeruginosa strain that produces higher concentrations phenazines under conditions over-expressing PqsE effector negative ΔpqsC mutant. engineered exhibited improved electrical performance microbial fuel cells (MFCs) and...
The mechanism by which extracellular metabolites, including redox mediators and quorum-sensing signaling molecules, traffic through the matrix of biofilms is poorly explored. We hypothesize that functional amyloids, abundant in natural possessing hydrophobic domains, retain these metabolites. Using surface plasmon resonance, we demonstrate (QS) 2-heptyl-3-hydroxy-4(1H)-quinolone N-(3-oxododecanoyl)-l-homoserine lactone, mediator pyocyanin bind with transient affinity to amyloids from...