A5014814934- Mesenchymal stem cell research
- Nerve injury and regeneration
- Prion Diseases and Protein Misfolding
- Spinal Cord Injury Research
- Wound Healing and Treatments
- Reproductive Physiology in Livestock
- Cervical and Thoracic Myelopathy
- Neurogenesis and neuroplasticity mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Veterinary Equine Medical Research
- Aortic Disease and Treatment Approaches
- Neonatal and fetal brain pathology
- Mechanical Circulatory Support Devices
- Gut microbiota and health
- Ocean Acidification Effects and Responses
- Phagocytosis and Immune Regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Reproductive System and Pregnancy
- Trace Elements in Health
- Pressure Ulcer Prevention and Management
- COVID-19 Clinical Research Studies
- Muscle Physiology and Disorders
- Complement system in diseases
- Amino Acid Enzymes and Metabolism
- Cancer-related Molecular Pathways
Fondazione IRET ONLUS
2019-2024
University of Bologna
2008-2020
Chiesi (Italy)
2020
In prion disease, the abnormal conformer of cellular protein, PrPSc, deposits in fibrillar protein aggregates brain and other organs. Limited exposure PrPSc to proteolytic digestion vitro generates a core fragment 19–21 kDa, named PrP27–30, which is also found vivo. Recent evidence indicates that truncated fragments than PrP27–30 may form disease either vivo or vitro. We characterized novel protease-resistant PrP migrating 2–3 kDa faster Creutzfeldt-Jakob (CJD) brains. The has size about...
Six clinico-pathological phenotypes of sporadic Creutzfeldt–Jakob disease have been characterized which correlate at the molecular level with type (1 or 2) abnormal prion protein, PrPTSE, present in brain and genotype polymorphic (methionine valine) codon 129 protein gene. However, to what extent these their corresponding combinations (i.e. MM1, MM2, VV1 etc.) encipher distinct strains upon transmission remains uncertain. We studied PrPTSE gene archival tissues from National Institutes...
Experimental models for chronic skin lesions are excision and pressure ulcer, defined as "open" "closed" lesions, respectively, only the latter characterized by tissue hypoxia. Moreover, systemic diseases, such diabetes mellitus, affect wound repair. Thus, testing new therapies should be carefully selected according to expected targets. In this study, we present an extensive comparative histological, immunohistochemical, molecular characterization of these two in diabetic (db/db)...
Nerve growth factor (NGF) is a pleiotropic molecule acting on different cell types in physiological and pathological conditions. However, the effect of NGF survival, differentiation maturation oligodendrocyte precursor cells (OPCs) oligodendrocytes (OLs), responsible for myelin formation, turnover, repair central nervous system (CNS), still poorly understood heavily debated.Here we used mixed neural stem (NSC)-derived OPC/astrocyte cultures to clarify role throughout entire process OL...
ABSTRACT The wide phenotypic variability of prion diseases is thought to depend on the interaction a host genotype with strains that have self-perpetuating biological properties enciphered in distinct conformations misfolded protein PrP Sc . This concept largely based indirect approaches studying effect proteases or denaturing agents physicochemical aggregates. Furthermore, most data come from studies rodent-adapted strains, making current understanding molecular basis and naturally...
Dogs with myxomatous mitral valve disease (MMVD) can experience progressive renal tubular damage and dysfunction. The prevalence of is not known in dogs stable MMVD.To evaluate MMVD by evaluation urinary neutrophil gelatinase-associated lipocalin (NGAL).Ninety-eight grouped according to the American College Veterinary Internal Medicine (ACVIM) staging (group B1, n = 23; group B2, 27; C + D, 48) 46 healthy dogs.Multicenter prospective observational study. Serum urine chemistry including NGAL...
Abstract Systemic inflammation and neuroinflammation affect the natural course of sporadic form Alzheimer’s disease (AD), as supported by epidemiological preclinical data, several studies indicate a higher prevalence AD in patients with inflammatory bowel disease. In this study, we explored whether colitis induced dextran sulfate sodium (DSS) young, presymptomatic/preplaque mice worsens and/or anticipates age-dependent cognitive impairment Tg2576, widely used mouse model AD. We demonstrated...
Abstract Background Greater knowledge of mesenchymal stromal cell (MSC)-based therapies is driving the research into their secretome, identified as main element responsible for therapeutic effects. The aim this study to characterize individual variability secretome adipose tissue-derived MSCs (adMSCs) with regard potential therapeutical applications in neurology. Methods adMSCs were isolated from intact tissue ten subjects undergoing abdominal plastic surgery or reduction mammoplasty. Two...
Spinal cord injury (SCI) is characterized by a cascade of events that lead to sensory and motor disabilities. To date, this condition irreversible, no cure exists. improve myelin repair limit secondary degeneration, we developed multitherapy based on nanomedicines (NMeds) loaded with the promyelinating agent triiodothyronine (T3), used in combination systemic ibuprofen mouse nerve growth factor (mNGF). Poly-L-lactic-co-glycolic acid (PLGA) NMeds were optimized T3 promote sustained release....
The lack of reliable diagnostic and prognostic markers for spinal cord injured (SCI) patients is a severe obstacle in development testing new therapies, it also impairs appropriate rehabilitation care. sparse available data on the biochemical composition cerebrospinal fluid (CSF) during acute and/or chronic phase lesion provide, up until now, inconsistent results. In this pilot study, we then explored possibility combining multi-parametric bioinformatic analysis CSF its biological properties...
The involvement of the extracellular matrix (ECM) in lesion evolution and functional outcome is well recognized spinal cord injury. Most attention has been dedicated to "core" area scar formation, while only scattered reports consider ECM modification based on temporal segments adjacent lesion. In this study, we investigated expression profile 100 genes encoding for proteins at 1, 8 45 days post-injury, rostral caudal segment, a rat model. During both active phases stabilization, observed an...
Vascular and traumatic injuries of the central nervous system are recognized as global health priorities. A polypharmacology approach that is able to simultaneously target several injury factors by combination agents having synergistic effects appears be promising. Herein, we designed a polymeric delivery loaded with two drugs, ibuprofen (Ibu) thyroid hormone triiodothyronine (T3) in vitro release suitable amount anti-inflammation remyelination drug. As production method, electrospinning...
Nerve growth factor (NGF) is the protein responsible for development and maintenance of sensory skin innervation. Given role appropriate innervation in healing, NGF has been indicated as a possible prohealing treatment pathologic conditions characterized by nerve-ending loss, such chronic ulcers diabetes; however, its use therapeutic agent limited hyperalgesic effect. We tested effect topical application nonalgogenic derivative hNGFP61S/R100E two models ulcer induced <i>dbdb</i> diabetic...
Neonatal Encephalopathy (NE) may be caused by hypoxic ischemic insults or inflammatory and modified innate protective excitatory mechanisms. Understanding the underlying pathophysiology is important in formulating a rational approach to diagnosis. The preliminary aim was clinically characterize population of foals spontaneously affected NE. study aimed to: (i) evaluate nerve growth factor (NGF) vascular endothelial (VEGF) levels plasma samples obtained at parturition from mare’s jugular...
The importance of trophic factors, such as nerve growth factor (NGF), vascular endothelial (VEGF), and brain-derived neurotrophic (BDNF) during the perinatal period, is now emerging. Through their functional activities neurogenesis angiogenesis, they play a key role in final maturation nervous systems. present study aims to: (i) evaluate NGF VEGF levels obtained at parturition from mare, foal umbilical cord vein plasma, well amniotic fluid; (ii) content plasma healthy foals first 72 h life...