A5030171307- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Renin-Angiotensin System Studies
- Dendrimers and Hyperbranched Polymers
- Computational Drug Discovery Methods
- RNA and protein synthesis mechanisms
- Receptor Mechanisms and Signaling
- Virus-based gene therapy research
- Metabolism, Diabetes, and Cancer
- Hormonal Regulation and Hypertension
- Nitric Oxide and Endothelin Effects
- HER2/EGFR in Cancer Research
- biodegradable polymer synthesis and properties
- Cardiac Ischemia and Reperfusion
- Cardiac electrophysiology and arrhythmias
- Diabetes Treatment and Management
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Click Chemistry and Applications
- MicroRNA in disease regulation
- Neuropeptides and Animal Physiology
- Polyomavirus and related diseases
- Polymer crystallization and properties
- Monoclonal and Polyclonal Antibodies Research
Qatar University
2017-2025
Kuwait University
2008-2017
Cardiff University
2003-2017
Systems, Applications & Products in Data Processing (Canada)
2016
Nuffield Orthopaedic Centre
2007
University of Oxford
2007
Tabriz University of Medical Sciences
2005
Aston University
1995-2004
University of Bath
1977-1992
University of North Carolina at Chapel Hill
1991-1992
Journal Article Mechansism of cellular uptake modified oligodeoxynucleotides containing methylphosphonate linkages Get access Yoko Shoji, Shoji Search for other works by this author on: Oxford Academic PubMed Google Scholar Saghir Akhtar, Akhtar Ammasi Periasamy, Periasamy 1Department Cell Biology and Anatomy Lineberger Cancer Research Center, UNiversity NOrth CarolinaChapel Hill, NC 27599, USA Brian Herman, Herman R.L. Juliano * To whom corespondence should be addressed Nucleic Acids...
BACKGROUND AND PURPOSE Angiotensin-(1-7) [Ang-(1-7)] has anti-inflammatory effects in models of cardiovascular disease and arthritis, but its asthma are unknown. We investigated whether Ang-(1-7) actions a murine model asthma. EXPERIMENTAL APPROACH The alone or combination with the MAS1 receptor antagonist, A779, were evaluated over 4 day period an ovalbumin-challenged mouse allergic On 5, bronchoalveolar lavage was performed, lungs sectioned assessed histologically for quantification goblet...
Of the non-viral vectors, cationic lipid (CL) formulations are most widely studied for delivery of genes, antisense oligonucleotides and gene silencing nucleic acids such as small interfering RNAs. However, little is known about impact these systems on global expression in target cells. In an attempt to study geno-compatibility CL cells, we have used microarrays examine effect Lipofectin Oligofectamine profiles human A431 epithelial Using manufacturer's recommended concentrations routinely...
Polypropylenimine (PPI) dendrimers appear attractive non-viral vectors for the delivery of genes, antisense oligonucleotides, and small interfering RNA (siRNA). However, effects these synthetic gene on global expression are poorly understood. Here we have examined toxicogenomics generation 2 (DAB-8) 3 (DAB-16) PPI in two human cell lines. At concentrations treatment protocols routinely used oligonucleotide transfection, alone elicited marked changes endogenous A431 epithelial cells. The...
Journal Article Interactions of antisense DNa oliginucletide analogs with phospholid membranes (liposomes) Get access Saghir akhtar, akhtar + Present Address: Department Pharamacutical Sciences, Aston UNiversity, Triangle, BIrmingahm B4 7ET, UK Search for other works by this author on: Oxford Academic PubMed Google Scholar Soumitra Basu, Basu 1Department Chemistry, Biochemistry & Molecular Biology, and surgery, UNiversity South FloridaTampa, FL 33620, USA Eric Wickstrom, Wickstrom R.L....
Small interfering RNA (siRNA), antisense oligonucleotides (ODNs), ribozymes and DNAzymes have emerged as sequence-specific inhibitors of gene expression that may therapeutic potential in the treatment a wide range diseases. Due to their rapid degradation vivo, efficacy naked silencing nucleic acids is relatively short lived. The entrapment these within biodegradable sustained-release delivery systems improve stability reduce doses required for efficacy. In this study, we evaluated vitro vivo...
Synthetic siRNAs are typically formulated with drug delivery systems (DDS) that improve cellular uptake for optimal gene silencing activity. Here, we show two PAMAM dendrimer DDS, differing only in their structural architecture, elicit many different expression changes human cells including opposing effects on the of epidermal growth factor receptor (EGFR), a targeted by siRNA. Despite providing similar improvements siRNA uptake, these formulations led to ∼10-fold variation anti-EGFR These...
Abstract The molecular mechanisms underlying asthma pathogenesis are poorly characterized. In this study, we investigated (1) whether Src mediates epidermal growth factor receptor (EGFR) transactivation; (2) if ERK1/2, PI3Kδ/Akt and NF-κB signaling effectors downstream of Src/EGFR activation; (3) upstream inhibition is more effective in downregulating the allergic inflammation than selective pathways. Allergic resulted increased phosphorylation EGFR, Akt, ERK1/2 IκB lung tissues from...
This study characterized the effects of diabetes and/or ischemia on epidermal growth factor receptor, EGFR, erbB2 signaling pathways cardiac function. Isolated heart perfusion model global was used to effect chronic inhibition or acute activation EGFR/erbB2 function in a rat type-1 diabetes. Induction with streptozotocin impaired recovery (cardiac contractility and hemodynamics) following 40 minutes isolated hearts. Chronic treatment AG825 AG1478, selective inhibitors EGFR respectively, did...
The transactivation of the epidermal growth factor (EGF) receptor appears to be an important central transduction mechanism in mediating diabetes-induced vascular dysfunction. Angiotensin-(1-7) [Ang-(1-7)] via its Mas can prevent development hyperglycaemia-induced cardiovascular complications. Here, we investigated whether Ang-(1-7) inhibit EGF and classical signalling ERK1/2 p38 MAPK vivo vitro.Streptozotocin-induced diabetic rats were chronically treated with or AG1478, a selective...