A5038401553- Microbial Community Ecology and Physiology
- Microbial bioremediation and biosurfactants
- Genomics and Phylogenetic Studies
- biodegradable polymer synthesis and properties
- Subterranean biodiversity and taxonomy
- Environmental DNA in Biodiversity Studies
- Microplastics and Plastic Pollution
- Aquatic Invertebrate Ecology and Behavior
- Aquaculture Nutrition and Growth
- Methane Hydrates and Related Phenomena
- Microbial Metabolic Engineering and Bioproduction
- Protein Hydrolysis and Bioactive Peptides
- Marine Ecology and Invasive Species
- Animal Genetics and Reproduction
- Food Industry and Aquatic Biology
- Atmospheric and Environmental Gas Dynamics
- Building materials and conservation
- Protist diversity and phylogeny
- Algal biology and biofuel production
- Marine Biology and Ecology Research
- Carbon dioxide utilization in catalysis
- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- Anaerobic Digestion and Biogas Production
- Microbial Natural Products and Biosynthesis
University of Bucharest
2012-2023
Babeș-Bolyai University
2020-2023
Peking University
2017
Movile Cave (SE Romania) is a chemoautotrophically-based ecosystem fed by hydrogen sulfide-rich groundwater serving as primary energy source analogous to the deep-sea hydrothermal ecosystems. Our current understanding of microbiology has been confined sulfidic water and its proximity, most studies focused on water-floating microbial mat planktonic accumulations likely acting production powerhouse this unique subterranean ecosystem. By employing comprehensive genomic-resolved metagenomics, we...
High-throughput screening (HTS) methods for characterization of microbial production polyhydroxyalkanoates (PHA) are currently under investigated, despite the advent such systems in related fields. In this study, phenotypic microarray by Biolog PM1 Halomonas sp. R5-57 and Pseudomonas MR4-99 identified 49 54 carbon substrates to be metabolized these bacteria, respectively. Growth on 15 ( R5-57) 14 MR4-99) was subsequently characterized 96-well plates using medium with low nitrogen...
Karst caves are characterized by relatively constant temperature, lack of light, high humidity, and low nutrients availability. The diversity functionality the microorganisms dwelling in micro-habitats yet underexplored. Therefore, in-depth investigations these ecosystems aid enlarging our understanding microbial interactions microbially driven biogeochemical cycles. Here, we aimed at evaluating diversity, abundance, distribution, organic substrate preferences communities from Peștera cu Apă...
DNA isolation represents the basic and probably most important step in molecular biology for microbial strains, even more, community analyses. Despite development of protocols isolation, there are still many drawbacks dependent samples composition, commercially available genomic kits have significant limitations recovering high amounts, especially from soil samples. Our study is aiming to compare optimize a total protocol polluted samples, estimating amount purity per g soil, versus time...
Bacterial contamination during fuel storage can lead to biodegradation (biodeterioration) or infrastructure problems (i.e., microbiologically influenced corrosion (MIC), biofouling, etc.).Jet A-1 tank samples were screened, and 23 morphologically distinct bacterial strains isolated.6 isolates revealed biofilm initiation capacity.For 12 the alkane monooxygenase gene (alkB) was successfully detected.The alkB-possessing 16S rDNA sequences identified as belonging Bradyrhizobium, Sphingomonas,...
Introduction: Karstic springs are used worldwide by rural communities as sources of fresh water for humans and livestock. In Romania, one-third the population has no direct access to a public supply. The present study is part country-wide project develop simple, quick cheap methods seasonal environmental microbiological monitoring karstic drinking populations. Critical steps workflow consist evaluating quality selecting suitable membrane filters efficiently capture DNA further microbial...
Flow cytometry is recognized as a useful tool in environmental microbiology studies, regarding oil contamination sites. This study was focused on cytometric analysis of cellular growth P. fluorescens S7A strain, previously isolated from polluted soil, order to assess bacterial response different n-hexadecane and, respectively, standard concentration using two fluorescent tracers. In the same time, we measured cell by spectrophotometric and colorimetric method. Cytometry analysis, propidium...
Abstract Polyhydroxyalkanoates (PHAs) are biodegradable bioplastics that can be manufactured sustainably and represent a promising green alternative to petrochemical‐based plastics. Here, we describe the complete genome of new marine PHA‐producing bacterium— Photobacterium ganghwense (strain C2.2), which have isolated from Black Sea seashore. This isolate is psychrotolerant accumulates PHA when glycerol provided as main carbon source. Transmission electron microscopy, specific staining with...
(1) Introduction: Karstic springs are used worldwide by rural communities as sources of fresh water for human use and livestock survival. In Romania, 1/3 the population has no direct access to public supply. The present study is part a country-wide project on developing simple, quick cheap methods seasonal environmental microbiological monitoring karstic drinking supply populations. Critical steps workflow consist in evaluation quality choosing suitable membrane filters efficiently capture...
Understanding the microbial communities responses to biopile procedures is an essential step in field of ecology and bioremediation applications.In order estimate metabolic dynamics at a community level, we used Biolog Ecoplates.Assays showed that bacterial from all samples were capable utilizing great variety 31 carbon substrates.Shannon's diversity index values between 2.711±0.303 August 3.262±0.062in April, recording general variation 0.5 similar other studies.Statistical analysis good...
The release of xenobiotic compounds as petroleum and its derivatives still represents a problematic not fully manageable consequence various anthropogenic activities.Thus, endogenous microbial communities facing such pollutants are constantly adapting to new conditions, their evaluation being an important asset for environmental preservation.This study analyzes structural metabolic shifts in soil community following kerosene pollution.Metabolic profiles from Biolog EcoPlate, analyzed guild...
Abstract Background Movile Cave (Dobrogea, SE Romania) hosts a subterranean chemoautotrophically-based ecosystem supported by sulfidic thermal aquifer analogous to the deep-sea hydrothermal ecosystems. Our current understanding of microbiology has been confined water proximity (no more than 2 m distant), with most studies focusing on water-floating mat, which likely acts as primary production powerhouse in this ecosystem. To gain insightful information functioning ecosystem, we employed...
Polyhydroxyalkanoates (PHA) are promising biodegradable and biocompatible bioplastics, extensive knowledge of the employed bacterial strain’s metabolic capabilities is necessary in choosing economically feasible production conditions. This study aimed to create an in-depth view utilization Photobacterium ganghwense C2.2 for PHA by linking a wide array characterization methods: pathway annotation from complete genome, high-throughput phenotypic tests, biomass analyses through plate-based...
Background: Microbial secondary metabolites (SM), especially those produced by soil microorganisms, have been a valuable source of antibiotics, antitumor agents, pigments, growth-promoting substances, etc., with tremendous market potential. These molecules are encoded biosynthetic gene clusters (BGCs) within the bacterial genome. Their synthesis confers survival advantages facilitating chemical defense, interspecies communication, and adaptation to well-defined ecological niches. Caves,...