A5013939529- Vector-borne infectious diseases
- Botany and Plant Ecology Studies
- Viral Infections and Vectors
- CRISPR and Genetic Engineering
- Mosquito-borne diseases and control
- Integrated Water Resources Management
- Environmental DNA in Biodiversity Studies
- Genomics and Phylogenetic Studies
- Insect symbiosis and bacterial influences
- Vector-Borne Animal Diseases
- CAR-T cell therapy research
- Mollusks and Parasites Studies
- Salivary Gland Tumors Diagnosis and Treatment
- Identification and Quantification in Food
- Toxoplasma gondii Research Studies
- Freshwater macroinvertebrate diversity and ecology
- Peatlands and Wetlands Ecology
- Insects and Parasite Interactions
- Bone and Dental Protein Studies
- Fossil Insects in Amber
- Polar Research and Ecology
- Genetic diversity and population structure
- Insect and Pesticide Research
- Peptidase Inhibition and Analysis
- Historical Geography and Cartography
Natural History Museum
2021-2025
University College London
2024
Great Ormond Street Hospital
2024
The Pirbright Institute
2023
Medical University of Silesia
2010-2020
University of Silesia in Katowice
2011-2017
University of Gdańsk
2016
Górnośląskie Centrum Medyczne
2012
Wojewódzki Szpital Specjalistyczny nr 5 im. św. Barbary w Sosnowcu
2010
Biodiversity genomics research requires reliable organismal identification, which can be difficult based on morphology alone. DNA-based identification using DNA barcoding provide confirmation of species identity and resolve taxonomic issues but is rarely used in studies generating reference genomes. Here, we describe the development implementation for Darwin Tree Life Project (DToL), aims to sequence assemble high quality genomes all eukaryotic Britain Ireland. We present a standardised...
New genomic technologies, such as third generation sequencing (TGS), have enabled for high-throughput, rapid, and cost-effective data of non-model organisms, accelerating taxonomic identification studies contributing to conservation applications. Here, we present the first comparison two leading TGS providers, Pacific Biosciences (PacBio) Oxford Nanopore Technologies (ONT), purpose DNA barcoding, using morphological Sanger reference. We demonstrate that highest numbers successfully sequenced...
Tick-borne encephalitis (TBE) is among the most important vector borne diseases of humans in Europe and currently identified as a major health problem many countries. TBE endemic zones have expanded over past two decades, well number reported cases within areas. Multiple factors are ascribed for increased incidence TBE, including climatic change. The has also Norway decade, human cluster along southern coast Norway. In distribution prevalence virus (TBEV) tick populations largely unknown....
Molar incisor hypomineralization (MIH) is a dental condition that affects the enamel of permanent molars and/or incisors, often leading to tooth decay. Although several etiological hypotheses have come forward, including prenatal medical problems and postnatal illness, pathogenesis MIH yet unclear. Aimed at exploring epigenomic landscape this condition, we collected tissue from MIH-affected child an age-matched control patient investigated their DNA methylation status through in-depth...
Umbilical cord blood (UCB) T cells exhibit distinct naive ontogenetic profiles and may be an attractive source of starting for the production chimeric antigen receptor (CAR) cells. Pre-selection UCB-T on basis CD62L expression was investigated as part a machine-based manufacturing process, incorporating lentiviral transduction, CRISPR-Cas9 editing, T-cell expansion depletion residual TCReeeT This provided stringent mitigation against risk graft versus host disease (GVHD), combined with...
Ticks (Acari: Ixodida) are vectors of many pathogens i. a: Babesia microti [Bm] and Toxoplasma gondii [Tg]. In Poland, Ixodes ricinus [Ir] is the main vector both pathogens. This tick species transmitted by them a significant threat to human animal health.Detection protozoans Bm Tg in ticks collected Tarnogórski district area.The were from vegetation pets spring period their activity on territory district. The parasites preserved 70% ethanol. DNA was isolated ammonia method. detected...
A phenomenon of increasing resistance Candida spp. to azoles has been observed for several years now. One the mechanisms lack sensitivity is associated with CDR1, CDR2, MRD1 genes (their products are active transport pumps conditioning drug efflux from pathogen's cell), and ERG11 gene (encoding lanosterol 14α-demethylase). Test material was 120 strains albicans (60 resistant 60 susceptible azole drugs) obtained clinical samples. The first stage experiment assessed expression MDR1 by Q-PCR....
Abstract The requirement for large amounts of purified DNA limits many sequencing experiments, especially when seeking to avoid pre-amplification or using third generation technology sequence molecules directly. We wanted test the current nanopore input requirements and devised a set experiments evaluate extraction library preparation approaches low inputs. found an optimised bead beating approach combined with magnetic protocol, rather than traditional spin columns extraction, improved both...
Events, such as the COVID-19 pandemic, that rapidly impact global communication and travel have significant consequences for tourism industry, which is one of pillars development. We assess impacts crisis on Colca y Volcanes de Andagua UNESCO Global Geopark in Peru. The was established immediately prior to pandemic October 2019. instability government Peru during difficult living conditions high Andes, lack drinking water, cleaning agents, medical care, levels poverty, particularly geopark...
New genomic technologies, such as third generation sequencing (TGS), have enabled for high- throughput, rapid, and cost-effective data of non-model organisms, accelerating taxonomic identification studies contributing to conservation applications. Here, we focus on the comparison two leading TGS providers, Pacific Biosciences (PacBio) Oxford Nanopore Technologies (ONT), purpose DNA barcoding. We demonstrate that highest numbers successfully sequenced samples were achieved with ONT R10 & Q20+...
1. Hubbard MJ, Cann KJ, Baker A. Epidemiological studies of Borrelia burgdorferi infection in fresh and archived British ticks. In: Mitchell R, Horn DJ, Needham GR, Welbourn WC (eds.). Acarology IX Proceedings 1.Ohio: Biological Survey 1996. p.513–18. Google Scholar
The Polish Scientifi c Expedition has been carrying out research in the Colca Canyon and Valley of Volcanoes (South Peru) since 2003. National Park project, covering mentioned areas, is main purpose research. extent genesis Quaternary volcanic Andahua group leading theme investigation. study area surrounded with numerous Pliocene Pleistocene stratovolcanoes: Ampato (6,288 m a.s.l.), Chachani (6,057 multi-cone Coropuna (6,425 a.s.l.) others. Some them are still active, such as Misti (5,822 or...
Umbilical cord blood (UCB) T cells exhibit distinct naive ontogenetic profiles and may be an attractive source of starting for the production chimeric antigen receptor (CAR) cells. Pre-selection UCB-T on basis CD62L expression was investigated as part a machine-based manufacturing process, incorporating lentiviral transduction, CRISPR-Cas9 editing, T-cell expansion depletion residual TCRab This provided stringent mitigation against risk graft versus host disease (GVHD), combined with...
Molar incisor hypomineralization (MIH) is a qualitative developmental defect that affects the enamel tissue of permanent molars and can also occur in incisors. Enamel affected by MIH has reduced hardness, increased porosity, higher organic content than unaffected enamel. These characteristics predispose to accumulation bacteria prevalence caries lesions. Through groundbreaking metagenomic analysis buccal mucosal sample from patient with MIH, we explored intricacies its microbiome compared...
Abstract Genetic manipulation of Aedes aegypti is key to developing a deeper understanding this insects’ biology, vector-virus interactions and makes future genetic control strategies possible. Despite some advances, process remains laborious requires highly skilled researchers specialist equipment. Here we present two improved methods for in species. Use transgenic lines which express Cre recombinase allowed, by simple crossing schemes, germline or somatic recombination transgenes, could be...
Introduction: Genetic manipulation of Aedes aegypti is key to developing a deeper understanding this insects’ biology, vector-virus interactions and makes future genetic control strategies possible. Despite some advances, process remains laborious requires highly skilled researchers specialist equipment. Methods: Here we present two improved methods for in species. Use transgenic lines which express Cre recombinase plasmid-based method expressing PhiC31 when injected into early embryos....