Rapid antimicrobial susceptibility testing is needed to reduce prescription of inappropriate antibiotics. A rapid alternative standard culture-based determine reductions in cell viability using the LIVE/DEAD® BacLightTM Bacterial Viability Kit. We optimised kit protocol for this application, focusing on simplifying process by minimising steps involved and determining optimal analytical parameters fluorescence measurements from dyes SYTO 9 propidium iodide (PI). demonstrate that our...

Stable isotope ratios and trace elements are well-established tools that act as signatures of the product's environmental conditions agricultural processes; but they involve time, money, environmentally destructive chemicals. In this study, we tested for first time potential near-infrared reflectance spectroscopy (NIR) to estimate/predict elemental compositions origin verification coffee. Green coffee samples from two continents, 4 countries, 10 regions were analysed five (δ13C, δ15N, δ18O,...

Coffee is a target for geographical origin fraud. More rapid, cost-effective, and sustainable traceability solutions are needed. The potential of hyperspectral imaging-near-infrared (HSI-NIR) advanced machine learning models rapid non-destructive classification coffee was explored the first time (i) to understand sensitivity HSI-NIR across various scales (continental, country, regional), (ii) identify discriminant wavelength regions. analysis conducted on green beans from three continents,...

Aim: To evaluate a flow cytometry protocol that uses reference beads for the enumeration of live and dead bacteria present in mixture. Methods Results: Mixtures Escherichia coli with live:dead concentration ratios varying from 0 to 100% were prepared. These samples stained using SYTO 9 propidium iodide 6 μm added. Bacteria enumerated by agar plate counting. counting before treatment isopropanol. There is linear relationship between presented method counts (R2 = 0.99) E. 0.93) concentrations...

Changes in the composition of extracellular matrix (ECM) are characteristic injury or disease cartilage tissue. Various imaging modalities and biochemical techniques have been used to assess changes tissue but lack adequate sensitivity, case techniques, result destruction sample. Fourier transform near infrared (FT-NIR) spectroscopy has shown promise for study composition. In current NIR was identify contributions individual components spectra by assessment major components, collagen...

SYTO 9 is a fluorescent nucleic acid stain that widely used in microbiology, particularly for fluorescence microscopy and flow cytometry analyses. Fluorimetry-based analysis, i.e. analysis of intensity from bulk sample measurement, more cost effective, rapid accessible than or but requires application-specific calibration. Here we show the relevance food safety analysis. We stained four bacterial species to (Bacillus cereus, Escherichia coli, Salmonella enterica subspecies ser. Typhimurium,...

A rapid and easy method that takes advantage of an inexpensive portable fibre-based spectroscopic system (optrode) to determine the ratio live dead bacteria is proposed. Mixtures Escherichia coli with proportions live:dead cells varying from 0 100% were stained using SYTO 9 propidium iodide (PI) measured optrode. We demonstrated several approaches obtaining E. in a mixture both dead, analyses fluorescence spectra collected by To find suitable technique for predicting percentage sample, four...

Rapid identification and enumeration of bacteria are critical, given the surge antibiotic-resistance, global exchange food products, use for bioremediation, pharmaceutical, production. In response, a wide range methods being developed that can be broadly classified as nucleic acid-based, structure-based, mass spectrometry-based, optically based. Optical have generated interest potential rapid, non-destructive, high-throughput, amplification-free measurements require minimal sample...

The perceived benefit of functional foods in the prevention or mitigation degenerative diseases has stimulated growth food market. This perception is based on presence these specific molecules which have a positive pharmacological effect when consumed sufficient quantities (bioactive compounds). increasing market and consumer desire for quality products with health benefits created need efficient accurate analytical methods quantification bioactive compounds raw materials finished products....

Many animals extract, synthesize and refine chemicals for colour display, where a range of compounds structures can produce diverse palette. Feather colours, example, span the visible spectrum mostly result from pigments in five chemical classes (carotenoids, melanins, porphyrins, psittacofulvins metal oxides). However, pigment that generates yellow penguin feathers appears to represent sixth, poorly characterized class feather pigments. This class, here termed 'spheniscin', is displayed by...

The fluorescence spectrum of bacterially bound acridine orange (AO) was investigated to evaluate its use for the rapid enumeration bacteria. Escherichia coli ATCC 25922 samples were stained with 2 × 10−2, 10−3 or 10−4% w/v AO, followed by 3, 0 washing cycles, respectively, and spectra recorded using a fibre-based spectroscopic system. Independent component analysis used analyse spectral datasets each staining method. Bacterial concentration order magnitude classification models calculated...

This study investigated the geographical origin classification of green coffee beans from continental to country and regional levels. An innovative approach combined stable isotope trace element analyses with non-linear machine learning data analysis improve marker selection. Specialty sourced three continents, eight countries, 22 regions were analyzed by measuring five ratios (δ13 C, δ15 N, δ18 O, δ2 H, δ34 S) 41 elements. Partial least squares discriminant (PLS-DA) was applied integrated...

Adulteration of food products remains a security risk. Spices are components with high value per unit mass due to their desired flavour attributes and therefore economically worthwhile targets for adulteration. Vibrational spectroscopy techniques could be ideal detect adulterants benefits such as speed analysis. Near infrared (NIR) mid-infrared (mid-IR) spectra were used quantify the amount adulterant (buckwheat or millet) in ground black pepper. NIR calibration average individual...