A5007615248- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- NF-κB Signaling Pathways
- Graphene and Nanomaterials Applications
- Ubiquitin and proteasome pathways
- interferon and immune responses
- Korean Peninsula Historical and Political Studies
- CRISPR and Genetic Engineering
- Immune Response and Inflammation
- Biomedical Ethics and Regulation
- Epigenetics and DNA Methylation
- Telomeres, Telomerase, and Senescence
- Cancer-related molecular mechanisms research
- Genetics and Neurodevelopmental Disorders
- Head and Neck Surgical Oncology
- Genomics, phytochemicals, and oxidative stress
- Retinoids in leukemia and cellular processes
- RNA regulation and disease
- Glioma Diagnosis and Treatment
- Carbon Nanotubes in Composites
- Microtubule and mitosis dynamics
- Skin Protection and Aging
- Nanoparticles: synthesis and applications
- Glutathione Transferases and Polymorphisms
- Lung Cancer Research Studies
North Carolina State University
2010-2024
University of North Carolina at Chapel Hill
2009-2022
Indiana University School of Medicine
2007
Abstract LincRNA-p21 is a long noncoding RNA and transcriptional target of p53 HIF-1α. regulates gene expression in cis trans , mRNA translation, protein stability, the Warburg effect, p53-dependent apoptosis cell cycle arrest doxorubicin-treated mouse embryo fibroblasts. plays key role response skin keratinocytes to UVB-induced DNA damage by inducing apoptosis. In cancer development, mutation allows upon successive UVB exposures evade arrest. We hypothesized that lincRNA-p21 has functional...
Abstract Therapeutic targeting of specific genetic changes in cancer has proven to be an effective therapy and the concept synthetic lethality emerged. CCAAT/enhancer-binding protein-β (C/EBPβ), a basic leucine zipper transcription factor, important roles cellular processes including differentiation, inflammation, survival, energy metabolism. Using genetically engineered mouse model, we report that deletion C/EBPβ pre-existing oncogenic Ha-Ras skin tumors vivo resulted rapid tumor...
Squamous cell carcinomas (SCCs), including lung, head & neck, bladder, and skin SCCs often display constitutive activation of the KEAP1-NRF2 pathway. Constitutive is achieved through multiple mechanisms, activating mutations in NFE2L2 (NRF2). To determine functional consequences Nrf2 on SCC development, we assessed effects mutant
The bZIP transcription factor, C/EBPα is highly inducible by UVB and other DNA damaging agents in keratinocytes. C/EBPα-deficient keratinocytes fail to undergo cell cycle arrest G1 response UVB-induced damage mice lacking epidermal are susceptible skin cancer. mechanism through which regulates the checkpoint unknown. Here we report untreated have normal levels of cyclin-dependent kinase inhibitor, p21, however, UVB-treated up-regulate nuclear p21 protein despite up-regulation Cdkn1a mRNA...
Abstract The telomere specific shelterin complex, which includes TRF1, TRF2, RAP1, TIN2, TPP1 and POT1, prevents spurious recognition of telomeres as double-strand DNA breaks regulates telomerase repair activities at telomeres. TIN2 is a key component the complex that directly interacts with TRF2 TPP1. In vivo, large majority TRF1 are in but without POT1. Since knockdown also removes from telomeres, previous cell-based assays only provide information on downstream effects after loss...
Cadmium (Cd) is a ubiquitous environmental pollutant associated with wide range of health outcomes including cancer. However, obscure exposure sources often hinder prevention efforts. Further, although epigenetic mechanisms are suspected to link these associations, gene sequence regions targeted by Cd unclear. Aberrant methylation differentially methylated region (DMR) on the MEG3 that regulates expression cluster genes MEG3, DLK1, MEG8, MEG9 and DIO3 has been multiple cancers. In 287...
The fiber-like shape of multi-walled carbon nanotubes (MWCNTs) is reminiscent asbestos, suggesting they pose similar health hazards when inhaled, including pulmonary fibrosis and mesothelioma. Mice deficient in the tumor suppressor p53 are susceptible to carcinogenesis. However, chronic pathologic effect MWCNTs delivered lungs heterozygous (p53+/-) mice has not been investigated. We hypothesized that p53+/- would be lung development after exposure either tangled (t-) or rod-like (r-) MWCNTs....
p53 is activated by DNA damage and oncogenic stimuli to regulate senescence, apoptosis cell-cycle arrest, which are essential prevent cancer. Here, we utilized UVB radiation, a potent inducer of damage, p53, skin cancer investigate the mechanism CCAAT/enhancer binding protein-β (C/EBPβ) in regulating p53-mediated keratinocytes test whether deletion C/EBPβ epidermis can protect mice from UVB-induced UVB-treatment conditional knockout (CKOβ) increased protein levels enhanced p53-dependent...
The skin is the first line of defense to cutaneous microbes and viruses, epidermal keratinocytes play a critical role in preventing infection by viruses pathogens through activation type I interferon (IFN) response. Using RNAseq analysis, here we report that conditional deletion C/EBPβ transcription factor mouse epidermis (CKOβ mice) resulted upregulation IFNβ numerous keratinocyte interferon-stimulated genes (ISGs). expression cytosolic pattern recognition receptors (cPRRs), recognize viral...
Abstract The ability of cells to correctly respond DNA damage is a vital step in maintaining genome stability and suppressing tumorigenesis. C/EBP family transcription factors involved the regulation cell proliferation, growth arrest, differentiation, inflammation, senescence energy metabolism range types. Previously, we have shown that C/EBPα highly inducible by through p53-dependent mechanism has novel role G1 checkpoint. Furthermore, deficient display an impaired checkpoint inappropriate...