A5058891255- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Gut microbiota and health
- Bacteriophages and microbial interactions
- Mycobacterium research and diagnosis
- Probiotics and Fermented Foods
- Nail Diseases and Treatments
- Korean Urban and Social Studies
- Microbial Metabolism and Applications
- Fungal Infections and Studies
- Plant-Microbe Interactions and Immunity
- Clostridium difficile and Clostridium perfringens research
- Plant Pathogens and Fungal Diseases
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Diverse Topics in Contemporary Research
- Polar Research and Ecology
- Plant Pathogenic Bacteria Studies
- Escherichia coli research studies
- Microbial Metabolic Engineering and Bioproduction
- Antifungal resistance and susceptibility
- Urban and spatial planning
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Protist diversity and phylogeny
- Aquaculture disease management and microbiota
Kangwon National University
1996-2025
Seoul National University Bundang Hospital
2019-2024
Seoul National University
2012-2023
Korea Polar Research Institute
2017-2023
Institute for Basic Science
2022-2023
Inha University
2019
Sogang University
2013
Hanyang University
2011
Hyundai Motors (South Korea)
2011
Despite recent advances in commercially optimized identification systems, bacterial remains a challenging task many routine microbiological laboratories, especially situations where taxonomically novel isolates are involved. The 16S rRNA gene has been used extensively for this when coupled with well-curated database, such as EzTaxon, containing sequences of type strains prokaryotic species validly published names. Although the EzTaxon database widely isolates, from uncultured prokaryotes...
EzEditor is a Java-based molecular sequence editor allowing manipulation of both DNA and protein alignments for phylogenetic analysis. It has multiple features optimized to connect initial computer-generated alignment subsequent analysis by providing manual editing with reference biological information specific the genes under consideration. provides various functionalities rRNA using secondary structure information. In addition, it supports simultaneous sequences their translated...
Next-generation sequencing has great potential for application in bacterial transcriptomics. However, unlike eukaryotes, bacteria have no clear mechanism to select mRNAs over rRNAs; therefore, rRNA removal is a critical step sequencing-based Duplex-specific nuclease (DSN) an enzyme that, at high temperatures, degrades duplex DNA preference single-stranded DNA. DSN treatment been successfully used normalize the relative transcript abundance mRNA-enriched cDNA libraries from eukaryotic...
Infection by pathogenic viruses results in rapid epithelial damage and significantly impacts on the condition of upper respiratory tract, thus effects viral infection may induce changes microbiota. Thus, we aimed to define healthy microbiota pathogen-affected tract. In addition, any association between type agent resultant profile was assessed.We analyzed tract bacterial content 57 asymptomatic people (17 health-care workers 40 community people) 59 patients acutely infected with influenza,...
Objectives To investigate whether and how inflammatory disease in the intestine influences development of arthritis, considering that organ-to-organ communication is associated with many physiological pathological events. Methods First, mice were given drinking water containing dextran sodium sulfate (DSS) then subjected to arthritis. We compared phenotypic symptoms between cohoused separately-housed mice. Next, donor divided into DSS-treated untreated groups recipient Arthritis was induced...
Vibrio vulnificus is the causative agent of life-threatening septicemia and severe wound infections. Here, we announce complete annotated genome sequence V. MO6-24/O, isolated from a patient with septicemia. When it compared previously known genomes, this bacterium shows unique genetic makeup, including phagelike elements, carbohydrate metabolism-related genes, superintegron.
Members of the Mycobacterium abscessus complex are rapidly growing mycobacteria that emerging as human pathogens. The M. was previously composed three species, namely sensu stricto, 'M. massiliense', and bolletii'. In 2011, massiliense' bolletii' were united reclassified a single subspecies within abscessus: subsp. bolletii. However, placement boundary bolletii remains highly controversial with regard to clinical aspects. this study, we revisited taxonomic status members based on comparative...
Vanillin is a well-known food and cosmetic additive has antioxidant antimutagenic properties. It also been suggested to have antifungal activity against major human pathogenic fungi, although it not very effective. In this study, the activities of vanillin 33 derivatives fungal pathogen Cryptococcus neoformans, main cryptococcal meningitis in immunocompromised patients, were investigated. We found structural correlation between activity, showing that hydroxyl or alkoxy group more...
Abstract Dandruff is known to be associated with Malassezia restricta . Zinc pyrithione (ZPT) has been used as an ingredient in anti-dandruff treatments. The mechanism of ZPT investigated several studies; however, a non-pathogenic model yeast, such Saccharomyces cerevisiae was most often used. aim the present study understand how inhibits growth M. We analyzed cellular metal content and transcriptome profile ZPT-treated cells found that treatment dramatically increased zinc levels, along...
Malassezia species are opportunistic pathogenic fungi that frequently associated with seborrhoeic dermatitis, including dandruff. Most lipid dependent, a property is compensated by breaking down host sebum into fatty acids lipases. In this study, we aimed to sequence and analyse the whole genome of restricta KCTC 27527, clinical isolate from Korean patient severe dandruff, search for lipase orthologues identify most expressed on scalp patients The M. 27527 was sequenced using Illumina MiSeq...
The gut mycobiome plays an important role in the health and disease of human gut, but its exact function is still under investigation. While there a wealth information available on bacterial community microbiome, research fungal relatively limited. In particular, technical methodologies for analysis, especially DNA extraction method faecal samples, varied different studies. current study, two commercial kits commonly used extraction, QIAamp® Fast Stool Mini Kit DNeasy PowerSoil Pro Kit, one...
Escherichia coli J53 (F(-) met pro Azi(r)) is a derivative of E. K-12 which resistant to sodium azide. This strain has been widely used as general recipient for various conjugation experiments. Here, we report the genome sequence (=KACC 16628).
The full genome sequence of a Middle East respiratory syndrome coronavirus (MERS-CoV) was identified from cultured and isolated in Vero cells. viral has high similarity to 53 human MERS-CoVs, ranging 99.5% 99.8% at the nucleotide level.
Abstract The MgtC virulence protein from the intracellular pathogen Salmonella enterica is required for its intramacrophage survival and in mice this requirement of conserved several pathogens including Mycobacterium tuberculosis . Despite critical role within macrophages, only a few molecular targets have been identified. Here, we report that PhoR histidine kinase activates phosphate transport independently available concentration. A single amino acid substitution prevents binding to MgtC,...
Plant-pathogen interactions at early stages of infection are important to the fate interaction. Xanthomonas oryzae pv. (Xoo) causes bacterial blight, which is a devastating disease in rice. Although vivo and vitro systems have been developed study rice-Xoo interactions, both limitations. The resistance mechanisms rice can be better studied by approach, whereas suitable for pathogenicity studies on Xoo. current system uses minimal medium activate pathogenic signal (expression...
Malassezia is the most dominant fungal genus on human skin surface and associated with various diseases including dandruff seborrheic dermatitis. Among species, restricta widely observed species skin. In current study, we identified a novel dsRNA virus, named MrV40, in M. characterized sequence structure of viral genome along an independent satellite segment. Moreover, expression genes involved ribosomal synthesis programmed cell death was altered, indicating that virus infection affected...
Abstract Conversion of sunlight and organic carbon substrates to sustainable energy sources through microbial metabolism has great potential for the renewable industry. Despite recent progress in photosynthesis, development platforms that warrant efficient scalable fuel production remains its infancy. Efficient transfer retrieval gaseous reactants products from microbes are particular hurdles. Here, inspired by water lily leaves floating on water, a device designed operate at air–water...
Bacterial cell wall assembly and remodeling require activities of peptidoglycan (PG) hydrolases as well PG synthases. In particular, the activity DD-endopeptidases, which cleave 4-3 peptide crosslinks in PG, is essential for expansion gram-negative bacteria. Maintaining optimal levels DD-endopeptidases critical expanding without compromising its integrity. Escherichia coli , major MepS MepH, along with lytic transglycosylase MltD, are controlled by periplasmic protease Prc outer membrane...