A5090746853- 3D Printing in Biomedical Research
- Cell Image Analysis Techniques
- Retinal Development and Disorders
- Microfluidic and Bio-sensing Technologies
- Traumatic Brain Injury and Neurovascular Disturbances
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Neuroscience and Neural Engineering
- RNA Interference and Gene Delivery
- Cerebrospinal fluid and hydrocephalus
- Parkinson's Disease Mechanisms and Treatments
- Musculoskeletal pain and rehabilitation
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Neurological disorders and treatments
- Protein Degradation and Inhibitors
- Pain Mechanisms and Treatments
- Microfluidic and Capillary Electrophoresis Applications
- Neurosurgical Procedures and Complications
- Extracellular vesicles in disease
- CRISPR and Genetic Engineering
- Photoreceptor and optogenetics research
King's College London
2024
Guy's Hospital
2024
University of Bristol
2019-2021
Newcastle University
2013-2015
Southend University Hospital NHS Foundation Trust
2015
Abstract We and others have previously demonstrated that retinal cells can be derived from human embryonic stem (hESCs) induced pluripotent under defined culture conditions. While both cell types give rise to derivatives in the absence of inductive cues, this requires extended periods gives lower overall yield. Further understanding innate differentiation ability, identification key factors drive process, development clinically compatible conditions reproducibly generate functional neural...
Abstract Cellular systems have evolved numerous mechanisms to adapt environmental stimuli, underpinned by dynamic patterns of gene expression. In addition transcription regulation, modulation protein levels, dynamics and localization are essential checkpoints governing cell functions. The introduction inducible promoters has allowed expression control using orthogonal molecules, facilitating its rapid reversible manipulation study function. However, differing stabilities hinder the...
Extracting quantitative measurements from time-lapse images is necessary in external feedback control applications, where segmentation results are used to inform algorithms. We describe ChipSeg, a computational tool that segments bacterial and mammalian cells cultured microfluidic devices imaged by microscopy, which can be also the context of control. The method based on thresholding uses same core functions for both cell types. It allows us segment individual high density devices, quantify...
Mouse embryonic stem cells (mESCs) have been shown to exist in three distinct pluripotent states (ground, naïve and primed states), depending on culture conditions. External feedback control strategies been, so far, mainly used automatically regulate gene expression bacteria yeast. Here, we exploit a microfluidics/microscopy platform segmentation external algorithms for the automatic regulation of pluripotency phenotypes mESCs. We show feasibility controlling, living mESCs, levels an...
Here, we present a protocol for isolating and culturing mouse photoreceptors in minimal, chemically defined medium free from serum. We describe steps retina dissection, enzymatic dissociation, photoreceptor enrichment, cell culture, extracellular vesicles (EVs) EV ultrastructural analysis. This protocol, which has been verified cultured cells derived multiple murine strains, allows the study of several aspects biology, including isolation nanotube formation. For complete details on use...
ABSTRACT Extracting quantitative measurements from time-lapse images is necessary in external feedback control applications, where segmentation results are used to inform algorithms. While such image applications have been previously reported, there the literature a lack of open-source and documented code for community. We describe ChipSeg, computational tool segment bacterial mammalian cells cultured microfluidic devices imaged by microscopy. The method based on thresholding uses same core...
Abstract Mouse embryonic stem cells (mESCs) have been shown to exist in three distinct pluripotent states (ground, naïve and primed states), depending on culture conditions. External feedback control strategies been, so far, mainly used automatically regulate gene expression bacteria yeast. Here, we exploit a microfluidics/microscopy platform segmentation external algorithms for the automatic regulation of pluripotency phenotypes mESCs. We show feasibility controlling, living mESCs, levels...