A5038751070- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Pneumonia and Respiratory Infections
- SARS-CoV-2 and COVID-19 Research
- Infectious Diseases and Tuberculosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Air Quality and Health Impacts
- Immunodeficiency and Autoimmune Disorders
- Vaccine Coverage and Hesitancy
- Virology and Viral Diseases
- T-cell and B-cell Immunology
- Pediatric health and respiratory diseases
- Immune cells in cancer
- Influenza Virus Research Studies
- Vitamin D Research Studies
- Immunotherapy and Immune Responses
- Antimicrobial Peptides and Activities
- COVID-19 Clinical Research Studies
- Respiratory Support and Mechanisms
- Inhalation and Respiratory Drug Delivery
- Medical Imaging and Pathology Studies
- Macrophage Migration Inhibitory Factor
- Diagnosis and treatment of tuberculosis
Instituto Nacional de Enfermedades Respiratorias
2016-2025
Association Clinique et Thérapeutique Infantile du Val de Marne
2023-2024
National Heart Lung and Blood Institute
2024
Universidad Santiago de Cali
2013-2019
Office of Infectious Diseases
2004
Case Western Reserve University
1998-2002
University Hospitals of Cleveland
2002
National Institute of Cardiovascular Diseases
1997-2000
The innate immune response in human tuberculosis is not completely understood. To improve our knowledge regarding the role of cathelicidin hCAP-18/LL37 to infection, we used immunohistochemistry, immunoelectron microscopy, and gene expression study induction production antimicrobial peptide A549 epithelial cells, alveolar macrophages (AM), neutrophils, monocyte-derived (MDM) after infection with Mycobacterium tuberculosis. We demonstrated that mycobacterial induced LL-37 all cells studied,...
Toll-like receptors (TLRs) are critical components in the regulation of pulmonary immune responses and recognition respiratory pathogens such as Mycobacterium Tuberculosis (M.tb). Through examination human alveolar macrophages this study attempts to better define expression profiles TLR2, TLR4 TLR9 lung compartment which yet still poorly defined.
Abstract Background CD33 is a membrane receptor containing lectin domain and cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) that able to inhibit cytokine production. expressed by monocytes, reduced expression of correlates with augmented production inflammatory cytokines, such as IL-1β, TNF-α, IL-8. However, the role in inflammation associated hyperglycemia diabetes unknown. Therefore, we studied secretion freshly isolated monocytes from patients type 2 diabetes. To...
Inhalation exposure to indoor air pollutants and cigarette smoke increases the risk of developing tuberculosis (TB). Whether ambient pollution particulate matter (PM) alters protective human host immune responses against Mycobacterium has been little studied. Here, we examined effect PM from Iztapalapa, a municipality Mexico City, with aerodynamic diameters below 2.5 μm (PM2.5) 10 (PM10) on innate antimycobacterial in alveolar type II epithelial cells A549 cell line. Exposure PM2.5 or PM10...
A role for the nucleotide‐binding oligomerization domain 2 (NOD2) receptor in pulmonary innate immune responses has recently been explored. In present study, we investigated that NOD plays human alveolar macrophage and determined its involvement response to infection with virulent M ycobacterium tuberculosis . Our results showed was expressed macrophages, significant amounts of IL ‐1β, ‐6, TNF ‐α were produced upon ligand recognition muramyldipeptide ( MDP ). ligation induced transcription...
There is still a need for safe, efficient, and low-cost coronavirus disease 2019 (COVID-19) vaccines that can stop transmission of severe acute respiratory syndrome 2 (SARS-CoV-2). Here we evaluated vaccine candidate based on live recombinant Newcastle virus (NDV) expresses stable version the spike protein in infected cells as well surface viral particle (AVX/COVID-12-HEXAPRO, also known NDV-HXP-S). This be grown embryonated eggs at low cost, similar to influenza vaccines, it administered...
ABSTRACT Patients with active tuberculosis (TB) have a stronger humoral but poorer cellular immune response to the secreted 30-kDa antigen (Ag) of Mycobacterium than do healthy household contacts (HHC), who presumably are more protected against disease. The basis for this observation was studied by examining Th1 (interleukin 2 [IL-2] and gamma interferon [IFN-γ])- Th2 (IL-10 IL-4)-type cytokines produced in Ag peripheral blood mononuclear cells (PBMC) from patients pulmonary TB ( n = 7) HHC...
Abstract T cell Ig and mucin domain 3 (Tim3) is an inhibitory molecule involved in immune tolerance, autoimmune responses, antiviral evasion. However, we recently demonstrated that Tim3 Galectin-9 (Gal9) interaction induces a program of macrophage activation results killing Mycobacterium tuberculosis the mouse model infection. In this study, sought to determine whether Tim3–Gal9 pathway plays similar role human pulmonary TB. We identified TB patients have reduced expression on CD14+...
Background Human tuberculosis caused by M. bovis is a zoonosis presently considered sporadic in developed countries, but remains poorly studied problem low and middle resource countries. The disease humans mainly attributed to unpasteurized dairy products consumption. However, transmission due exposure of infected animals has been also recognized. prevalence infection associated risk factors have insufficiently characterized among farm workers (DFW) exposed settings with poor control bovine...
Background: COVID-19 is an infectious disease caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Many patients require invasive mechanical ventilation (IMV) while others, even with failure, do not (NIMV). Therefore, we aimed to evaluate serum levels of MMP-7 and molecules related exhausted T-cells as potential biomarkers differentiate between IMV NIMV patients. Methods: 105 diagnosed confirmed RT-PCR for SARS-CoV-2 were divided into two groups according requirement...
ABSTRACT Alveolar macrophages constitute a primary defense against Mycobacterium tuberculosis , but they are unable to control M. without acquired T-cell immunity. This study determined the antigen-presenting cell function of murine alveolar and ability model mycobacterium, bovis BCG, modulate it. The majority (80 85%) expressed both CD80 (B7.1) CD11c, 20 30% coexpressed major histocompatibility complex II (MHC-II). Gamma interferon (IFN-γ) enhanced MHC-II not B7.1 expression. Naive or...
A coagglutination technique was established for the detection of lipoarabinomannan Mycobacterium tuberculosis in human serum samples and evaluated its utility diagnosis at Instituto Nacional de Enfermedades Respiratorias Mexico City. The test had a sensitivity 88% patients with sputum-smear-positive active pulmonary tuberculosis. negative acid-fast bacilli sputum 67%. Less favorable results were obtained AIDS tuberculosis, 57%. specificity control lung diseases different from healthy...
The availability of highly purified lipoarabinomannan from Mycobacterium tuberculosis in its native acylated, antigenic state allowed application to the serodiagnosis patients Republic Mexico. Antilipoarabinomannan immunoglobulin G antibodies sera 66 with pulmonary, miliary, and pleural lymphadenities were measured by using enzyme-linked immunosorbent assay against a control population healthy individuals, people histoplasmosis, lung diseases not caused mycobacteria. results pointed an...
Exposure to air pollution particulate matter (PM) and tuberculosis (TB) are two of the leading global public health challenges affecting low middle income countries. An estimated 4.26 million premature deaths attributable household an additional 4.1 outdoor annually. Mycobacterium (M.tb) infects a large proportion world's population with risk for TB development increasing during immunosuppressing conditions. There is strong evidence that such immunosuppressive conditions develop exposure,...
New approaches for improving tuberculosis (TB) control using adjunct host-directed cellular and repurposed drug therapies are needed. Autophagy plays a crucial role in the response to TB, variety of autophagy-inducing drugs that currently available various medical conditions may serve as an treatment pulmonary TB. Here, we evaluated potential loperamide, carbamazepine, valproic acid, verapamil, rapamycin enhance antimicrobial immune Mycobacterium (Mtb). Human monocyte-derived macrophages...
Tuberculosis (TB) and air pollution both contribute significantly to the global burden of disease. Epidemiological studies show that exposure household urban increase risk new infections with Mycobacterium tuberculosis (M.tb) development TB in persons infected M.tb alter treatment outcomes. There is increasing evidence particulate matter (PM) weakens protective antimycobacterial host immunity. Mechanisms by which PM may adversely affect M.tb-specific human T cell functions have not been...
Associations between urban (outdoor) airborne particulate matter (PM) exposure and TB potential biological mechanisms are poorly explored.To examine whether in vivo to outdoor PM Mexico City vitro PM2.5 (< 2.5 µm median aerodynamic diameter) alters human host immune cell responses Mycobacterium tuberculosis.Cellular toxicity (flow cytometry, proliferation assay (MTS assay)), M. tuberculosis phagocytosis (microscopy), cytokine-producing cells (Enzyme-linked absorbent spot (ELISPOT)),...
Abstract There is still a need for safe, efficient and low-cost coronavirus disease 2019 (COVID-19) vaccines that can stop transmission of severe acute respiratory syndrome 2 (SARS-CoV-2). Here we evaluated vaccine candidate based on live recombinant Newcastle virus (NDV) expresses stable version the spike protein in infected cells as well surface viral particle (AVX/COVID-12-HEXAPRO, also known NDV-HXP-S). This be grown embryonated eggs at low cost similar to influenza it administered...
Responses to mycobacterial and nonmycobacterial antigens were examined in bronchoalveolar cells (BAC) peripheral blood mononuclear (PBMC) from patients with active pulmonary tuberculosis (n=16) healthy subjects (n=23). DNA synthesis BAC (but not PBMC) was significantly increased response the purified protein derivative (PPD), antigen 85, mannose-capped lipoarabinomannan but antigens. The PPD also enriched alveolar lymphocytes (P<.05). frequency of interferon-gamma interleukin-4- or...
Abstract Although the identity of T cells involved in protection against Mycobacterium tuberculosis (Mtb) humans remain unknown, patients with pulmonary (TB) have reduced numbers Mtb-reactive, Vγ9+/Vδ2+ their blood and lungs. Here we determined whether this γδ loss is a consequence Mtb Ag-mediated activation-induced cell death (AICD). Using DNA polymerase-mediated dUTP nick translation labeling assay, 5% or less freshly isolated CD4+ αβ from normal healthy individuals TB were apoptotic....