A5073908411- Cancer-related Molecular Pathways
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Cancer Research and Treatments
- Protein Kinase Regulation and GTPase Signaling
- Clinical Laboratory Practices and Quality Control
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- Antimicrobial Peptides and Activities
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- Cell death mechanisms and regulation
- RNA modifications and cancer
- Reproductive Biology and Fertility
- Receptor Mechanisms and Signaling
- Ubiquitin and proteasome pathways
- Meta-analysis and systematic reviews
- DNA and Nucleic Acid Chemistry
- Pharmacogenetics and Drug Metabolism
- HER2/EGFR in Cancer Research
- Peptidase Inhibition and Analysis
- Cancer Genomics and Diagnostics
- Computational Drug Discovery Methods
- Molecular Biology Techniques and Applications
SUNY Downstate Health Sciences University
2014-2024
Optimus Clinical Research
2021
New York University
1986-2018
State University of New York
1994-2017
Drexel University
2015-2017
Division of Chemistry
2017
VA NY Harbor Healthcare System
2007-2016
New York Proton Center
2013
American Museum of Natural History
2009
Rockefeller University
2009
Activation of the tumor suppressor p53 by stress and damage stimuli often correlates with induction kinases, Jun-NH 2 kinase (JNK). As JNK association plays an important role in stability, present study we have elucidated relationship between JNK-signaling pathway stability activity. Expression a constitutively active form JNKK upstream kinase, mitogen-activated protein (ΔMEKK1), increased level exogenously transfected null (10.1) cells as well endogenous MCF7 breast cancer cells. Increased...
A two-stage procedure for the determination of a united-residue potential designed protein simulations is outlined. In first stage, long-range and local-interaction energy terms total polypeptide chain are determined by analyzing protein-crystal data averaging all-atom surfaces. second stage (described in accompanying article), relative weights optimized so as to locate native structures selected test proteins lowest structures. The goal work present study parameterize physically reasonable...
The p53 tumor suppressor protein plays a key role in the regulation of stress-mediated growth arrest and apoptosis. Stress-induced phosphorylation tightly regulates its stability transcriptional activities. Mass spectrometry analysis phosphorylated 293T cells by active Jun NH2-terminal kinase (JNK) identified T81 as JNK site. at response to DNA damage stress-inducing agents, determined phospho-specific antibodies T81. Unlike wild-type p53, stimuli mutated on (T81A) did not exhibit increased...
Continuing our work on the determination of an off-lattice united-residue force field for protein-structure simulations, we determined and parameterized appropriate functional forms local-interaction terms, corresponding to rotation about virtual bonds (Utor), bending virtual-bond angles (Ub), energy different rotameric states side chains (Urot). These terms were by applying Boltzmann principle distributions torsional side-chain states, respectively, calculated from a data base 195...
Based on the dipole model of peptide groups developed in our earlier work [Liwo et al., Prot. Sci., 2, 1697 (1993)], a cumulant expansion average free energy system freely rotating peptide-group dipoles tethered to fixed α-carbon trace is derived. A graphical approach presented find all nonvanishing terms cumulants. In particular, analytical expressions for three- and four-body (correlation) averaged interaction potential united are These similar cooperative forces hydrogen bonding...
Based on the concept that hydrophobic interactions cause a polypeptide chain to adopt compact structure, method is proposed predict structure of protein. The procedure carried out in four stages: (1) use virtual-bond united-residue approximation with side chains represented by spheres search conformational space extensively using specially designed lead collapsed (2) conversion lowest-energy one real backbone, optimization hydrogen-bond network among backbone groups, (3) perturbation latter...
In addition to allergy and parasitic infections, immunoglobulin E (IgE) has been shown recently possess anti-viral anti-cancer effects. We investigated serum levels of IgE, its low-affinity receptor, soluble CD23 (sCD23) in patients with pancreatic cancer the effect IgE against cells. Twelve were evaluated for by imaging confirmed biopsy. Fifteen healthy volunteers served as controls. Serum Igs (IgG, IgM, IgA, IgE) sCD23 determined (enzyme-linked immunosorbent assay, nephelometry) presence...
The structure of the NH2-terminal, 20-residue membrane-bound portion melittin has been computed with empirical energies (ECEPP, Empirical Conformational Energy Program for Peptides). First, a search was made long stretches nonpolar residues. Then, low-energy conformations these segments were built up by combining successively their component di- and tripeptides. minimum-energy each peptides used in this buildup process selected so that had distinct backbone conformation; designated as...
The negative impact of continued school closures during the height COVID-19 pandemic warrants establishment cost-effective strategies for surveillance and screening to safely reopen monitor potential in-school transmission. Here, we present a novel approach increase availability repetitive routine testing that may ultimately reduce overall viral burden in community.We implemented program using SalivaClear࣪ pooled method included students, faculty staff from K-12 schools (student age range...
Phosphorylation of the p53 tumor suppressor protein is known to modulate its functions. Using bacterially produced glutathione S -transferase (GST)-p53 fusion and baculovirus-expressed histidine-tagged ( His p53), we have determined human phosphorylation by purified forms jun-N-kinase (JNK), kinase A (PKA), β subunit casein II (CKIIβ) as well kinases present in whole cell extracts (WCEs). We demonstrate that PKA potent kinase, albeit, a conformation- concentration-dependent manner, concluded...
Obesity worsens cancer-specific survival and all-cause mortality for women diagnosed with breast cancer. Rich in adipose tissue, the exhibits increased adipocyte size obesity, which correlates poor prognosis. However, is highly heterogeneous as tissue expands through both hyperplasia hypertrophy; can increase independently of weight gain. Despite these observations, impact on cancer cell behavior remains unclear due to insufficient approaches isolate adipocytes based maintain them culture...
Abstract An algorithm is proposed for the conversion of a virtual‐bond polypeptide chain (connected C α atoms) to an all‐atom backbone, based on determining most extensive hydrogen‐bond network between peptide groups while maintaining all backbone atoms in energetically feasible conformations. Hydrogen bonding represented by aligning peptide‐group dipoles. These are not contiguous amino acid sequence. The first dipoles be aligned those that both sufficiently close space arranged...
We have synthesized three peptides from the mdm-2 binding domain of human p53, residues 12–26 (PPLSQETFSDLWKLL), 12–20, and 17–26. To enable transport across cell membrane at same time to maximize active α-helical conformation for these peptides, each was attached its carboxyl terminus penetratin sequence, KKWKMRRNQFWVKVQRG, that contains many positively charged stabilize an α-helix when present on terminal end. All were cytotoxic cancer cells in culture, whereas a control, unrelated peptide...
The three-dimensional structures of the transforming region product EJ/T24 human bladder oncogene and c-Ha ras-1 gene have been calculated by using conformational energy calculations. These two genes, representing a its normal cellular homologue, encode 21,000-dalton peptides that differ one amino acid at position 12. We therefore examined energetically allowed conformations hydrophobic decapeptide surrounding this substitution site. calculations show most favorable form exists when...
Abstract Conformational energy calculations were performed on monosaccharide and oligosaccharide inhibitors substrates of lysozyme to examine the preferred conformations these molecules. A grid‐search method was used locate all low‐energy conformational regions for N ‐acetyl‐β‐ D ‐glycosamine (NAG), minimization then carried out in each regions. Three stable positions ‐acetyl group have ben located, two which plane amide unit is normal mean pyranosyl ring. Nine local minima located —CH 2 OH...
RAS gene-encoded p21 protein has been found to increase in vitro phosphorylation of JUN via its kinase, N-terminal kinase (JNK). This effect is mediated by increased JNK the presence wild-type and oncogenic (Val-12) a dose-dependent manner. Oncogenic more potent mediating this than normal counterpart. Both proteins bind purified that present cell extracts from transformed fibroblasts melanoma cells. have also bacterially expressed protein. binding dose dependent, enhanced GTP, depends on...