A5037845661- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Molecular Biology Techniques and Applications
- Plant Molecular Biology Research
- Turfgrass Adaptation and Management
- Legume Nitrogen Fixing Symbiosis
- Genetically Modified Organisms Research
- Plant Disease Resistance and Genetics
- Photosynthetic Processes and Mechanisms
- Plant Parasitism and Resistance
- Plant Genetic and Mutation Studies
- Plant nutrient uptake and metabolism
- Genetic Mapping and Diversity in Plants and Animals
- Alkaline Phosphatase Research Studies
- Toxin Mechanisms and Immunotoxins
- Plant Pathogenic Bacteria Studies
- Actinomycetales infections and treatment
- Genomics and Chromatin Dynamics
- Ocular Disorders and Treatments
- Research in Cotton Cultivation
- Plant Gene Expression Analysis
Central University of Kerala
2015-2024
Quaid-i-Azam University
2024
Indian Institute of Technology Madras
2013-2015
Madurai Kamaraj University
2008-2014
Background: Hospital-acquired infections (HAIs) represent a significant healthcare challenge globally, with Staphylococcus aureus (S. aureus) being leading cause of skin and soft tissue infections. Methicillin-resistant (MRSA) poses an even greater threat due to its resistance commonly used antibiotics. Understanding the prevalence patterns MRSA is crucial developing effective infection control strategies, especially in resource-limited settings like Peshawar, Pakistan. Objective: To...
Transgenic rice (Oryza sativa L. subsp. indica cv. White Ponni) constitutively expressing the thaumatin-like protein gene (tlp-D34, PR-5) individually or in combination with chitinase (chi11, PR-3) was generated using an Agrobacterium vir helper strain multiple copies of pTiBo542 virB and virG. Transformation tlp-D34 alone + chi11 genes yielded five seven single-copy transgenic lines, respectively. Southern blot analysis two probes, one flanking right T-DNA border second left border,...
Gene expression analysis using real-time polymerase chain reaction (PCR) requires the validation of a stable reference gene for normalization. Previously reported candidates may not be suitable novel crops. Vigna radiata is protein-rich legume crop used in many countries across world. As this recalcitrant to transformation such as most legumes, we were interested V. radiata-Agrobacterium interaction studies. Toward end, wanted identify normalization under Agrobacterium-infections conditions....
The DIANTHIN gene encoding a ribosome-inactivating protein (RIP) from Dianthus caryophyllus L. was tested for negative selection in tobacco and rice. Tobacco leaf discs scutellum-derived callus of rice were transformed with Agrobacterium tumefaciens strain LBA4404 (pSB1, pJAS1). pJAS1 harbors the under control CaMV 35S promoter. transformation efficiency, comparison to pCAMBIA1301, reduced by 87 % pJAS1-transformed discs. proved be completely toxic as all recovered hygromycin-resistant...
Agrobacterium infection, which is widely used to generate transgenic plants, often accompanied by T-DNA-linked mutations and transpositions in flowering plants. It not known if infection also affects the rates of point mutations, somatic homologous recombinations (SHR) frame-shift (FSM). We examined effects on five types using a set mutation detector lines Arabidopsis thaliana. To verify effect secreted factors, we exposed plants different strains, including wild type (Ach5), its derivatives...
Gametophyte-specific promoters drive the expression of genes in male and/or female gametophytes. These have applications breeding experiments, gene function identification, developmental biology-related studies, and off-lately genome editing also. The Plant Intracellular Ras-group Leucine-Rich-Repeat 6 (PIRL6) is known to be necessary for gametogenesis. Using GUS-based deletion analysis, we identified PIRL6 promoter length that essential exclusive gametophyte Arabidopsis thaliana. We studied...
Pesticides are known to induce genome instability. Report on eukaryotes, including humans, mostly covers chromosomal aberrations and DNA strand breaks. Studies the scoring of rates loci-specific somatic mutations such as point (PM), homologous recombination (SHR), frameshift mutation (FSM), transpositions due pesticides confined prokaryotes using Ames test. Analogous eukaryotic model systems though available for Drosophila, mouse certain cell lines, reports these meagre possibly lethality...
Leber congenital amaurosis (LCA) is a onset severe form of inherited retinal dystrophy (IRD) and common cause pediatric blindness. Disease-causing variants in at least 14 genes are reported to predispose LCA phenotype. as an autosomal recessive disease. It can be isolated eye disorder or part syndrome, such Senior Loken Joubert syndrome. Sequencing studies from consanguineous populations have proven useful for novel identification; thus, the present study aimed explore genetic heterogeneity...
AMA Amritha P, Shah J. Essential role of the BRCA2B gene in somatic homologous recombination Arabidopsis thaliana. BioTechnologia. 2023;104(4):371-380. doi:10.5114/bta.2023.132773. APA Amritha, P., & Shah, (2023). BioTechnologia, 104(4), 371-380. https://doi.org/10.5114/bta.2023.132773 Chicago Padinhatta Purayil, and Jasmine M. Shah. 2023. "Essential thaliana". BioTechnologia 104 (4): Harvard pp.371-380. MLA Purayil et al. thaliana." vol. 104, no. 4, 2023, pp. Vancouver