A5071100038- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Algal biology and biofuel production
- Food composition and properties
- Biofuel production and bioconversion
- Proteoglycans and glycosaminoglycans research
- Photosynthetic Processes and Mechanisms
- Microencapsulation and Drying Processes
- Gastrointestinal Tumor Research and Treatment
- Advanced Cellulose Research Studies
- Microbial Metabolites in Food Biotechnology
- Asthma and respiratory diseases
- Nanocomposite Films for Food Packaging
- Dermatology and Skin Diseases
- Glycosylation and Glycoproteins Research
- Plant Gene Expression Analysis
- Ginkgo biloba and Cashew Applications
- Phytochemicals and Antioxidant Activities
- GABA and Rice Research
- Microbial Natural Products and Biosynthesis
- biodegradable polymer synthesis and properties
- Advanced Sensor and Energy Harvesting Materials
- Marine and coastal ecosystems
- Proteins in Food Systems
- Fungal and yeast genetics research
Nanyang Technological University
2014-2023
National University Hospital
2022
Agency for Science, Technology and Research
2018
Institute of Medical Biology
2011-2018
National University Health System
2016
Abstract With increasing resource shortage and environmental pollution, it is preferable to utilize materials which are sustainable biodegradable. Side-streams products generated from the food processing industry one potential avenue that can be used in a wide range of applications. In this study, by-product okara was effectively reused for extraction cellulose. Then, cellulose further employed fabricate hydrogels with favorable mechanical properties, biodegrablability, non-cytotoxicity. The...
Okara is a major agro-waste, generated as byproduct from the soymilk and tofu industry. Since okara has high nutritive value, reusing it substrate for solid state biofermentation an economical environmental friendly option. Rhizopus oligosporus Lactobacillus plantarum were probiotic FDA-approved food-grade cultures used in this study. The study revealed that biofermenting improves its nutritional composition. It was found metabolomic composition (by GC-MS analysis) antioxidant activity DPPH...
Despite the exploration towards environmentally friendly manufacturing process, two main challenges remain for lactic acid synthesis by microbial fermentation: resource cost and production efficiency. To alleviate environmental issues, lower raw material costs, increase efficiency, this work aimed to evaluate characteristics of jackfruit seed starch (JFSS) extracted from fruit waste explore efficiency generation co-fermentation L. plantarum B. subtilis in JFSS. JFSS consisted a high amount...
Carotenoid production from three strains of Rhodosporidium toruloides grown on glycerol was studied. A time-dependent metabolomics approach used to understand its metabolism and mechanism for carotenoid in during different growth phases (1, 4, 7, 12 days). Strain CBS 5490 the highest producer (28.5 mg/L) had a unique metabolic profile. In this strain, metabolites belonging TCA cycle amino acids were produced lower amounts, as compared other strains. On hand, it amounts fatty acid...
Brewers' spent grain (BSG) is a by-product generated from the beer manufacturing industry, which extremely rich in protein and fiber. Here we use low cost BSG as raw material for production of novel growth media, through bioconversion process utilizing food grade fungi to hydrolyze BSG. The fermentation media was tested on yeast Rhodosporidium toruloides, natural producing carotenoid. analysed using curve intracellular fatty acids carotenoids. Untargeted GCMS based metabolomics used analyse...
Animal serum such as fetal bovine (FBS) is a commonly used irreplaceable essential supplement in cell culture media. However, the use of FBS has associated ethical, biosafety, and cost concerns industrial limits application cultivated meat production. Okara an agro-waste generated huge abundance daily, which can be biotransformed by fermentation. Different fractions fermented okara extracts were prepared ultrafiltration according to their molecular weight termed unfractionated, 50 kDa, 10 3...
Utilization of algicidal bacteria as a biological agent have been receiving significant interest for controlling harmful algal blooms. While various bacterial strains identified, limited studies explored the influence culture conditions on its activity. Here, effect oxygen activity novel bacterium JK12, against model diatom, Phaeodactylum tricornutum (P. tricornutum) was studied. Strain JK12 showed high P. and identified Pseudomonas chlororaphis chlororaphis) by 16S ribosomal RNA gene...
Soybean residue (okara) is an agricultural by-product, which rich in protein and fiber. This study evaluated a novel sequential process combined fungal pretreatment (F) twin screw extruder (E), to hydrolyze okara. The sequence of the steps, at speeds 200 rpm (200) or 600 (600), were tested. Next, soluble nutrients extracted create Fokara, EFokara200, EFokara600, FEokara200 FEokara600 okara media.All prepared media could support growth carotenoid production by yeast Rhodosporidium toruloides....
The search for natural and sustainable biopolymers is increasing. Biopolymers are used in applications, such as to encapsulate stabilize probiotic bacteria. Currently, this process requires many steps, including the separation of bacteria from culture media after fermentation, which energy intensive. In study, we developed a strategy use biopolymer durian seed, seed gum (DSG), address these issues. DSG was serve dual roles, firstly nutrient source, secondly an encapsulating agent. synergy...
Abstract Waste cooking oil (WCO) is a problematic waste product that contains free fatty acids (FFAs), preventing it from being valorized easily as biodiesel and poses an environmental hazard if incorrectly disposed. The use of WCO carbon source for Rhodosporidium toruloides ( R. ) using two‐phase culture system developed. normal growth when cultured in (OD 600 52) reveals its ability to hydrophobic substrate the compared glucose 51.9). Interestingly, extracellular lipase activity grown on...
A blend of β-glucans and anthocyanins lowers the digestibility glycemic index white rice.
Summary Nejayote is the wastewater obtained from maize lime cooking. It was used for Aspergillus oryzae growth and enzyme production. Protein production collagenolytic activity were evaluated to test effects of pH, spore inoculum nejayote content in a 3 factorial design. No observed supernatant fermentation without nejayote. Using at 5% increased 1.5 times fungus comparison with 1% 3%; maximum reached after days pH 8 ( P < 0.05). After sequencing, 32 kDa alkaline protease identified....
Food preservatives are ubiquitously used to increase the shelf life of manufactured foods. Current synthetic offer negligible health benefits, while healthier alternatives such as botanical extracts hindered by unsustainable production means. Here, we developed a natural, functional food preservative via combination sustainable technologies: food-grade yeast engineered produce natural plant compounds combined with emulsifiers valorized from milk whey and fruit pectin. Chemical compositional...
Flavonoids are valued for their beneficial properties. This was a proof-of-concept study to show that the PAL gene in Rhodosporidium toruloides (R. toruloides) could be harnessed and only two genes 4CL CHS needed inserted create naringenin producing strain, CBS-N. The growth of CBS-N reached higher OD600 86.1 compared 31.8 by wildtype. High-performance liquid chromatography (HPLC) chromatography–mass spectrometry (LC–MS) confirmed successfully produced 0.038 mg/L 16.9 p-coumaric acid via its...
Background Studies have reported that early febrile episodes and with infections are associated a decreased risk of developing atopy. Objective To examine further the association between presence number atopy atopic diseases if there was difference all fevers fever after vaccination. Methods We studied 448 infants in Singapore mother-offspring cohort study (Growing Up Towards Healthy Outcomes) which had complete data for exposures outcomes interest. Fever defined as more than 38.0°C...
Tang, Si Ying MRCP (UK); Wong, Neng Wei MRCS (Edinburgh); Lee, Jaslyn Jie Lin MBBS Author Information