Abstract: Microdialysis was used to assess extracellular dopamine in striatum, nucleus accumbens, and medial frontal cortex of unanesthetized rats both under resting conditions response intermittent tail‐shock stress. The metabolites 3,4‐dihydroxy‐phenylacetic acid homovanillic also were measured. concentration estimated be approximately 10 n M 11 3 cortex. In contrast, the levels 3,4‐dihydroxyphenylacetic low micromolar range. Intermittent stress increased relative baseline by 25% 39% 95%...

The murine monoclonal antibody (MAb), designated DF3, reacts with a 300,000-mol wt mammary epithelial antigen. A sequential double-determinant radioimmunoassay (RIA) has been developed to monitor circulating DF3 Using this assay, we have demonstrated that 33 of 36 normal women had plasma RIA antigen levels less than 150 U/ml. In contrast, 43 patients (76%) metastatic breast cancer greater or equal difference between these two groups was statistically significant (P 0.001). Similar results...

The abuse of psychostimulants, such as methamphetamine (METH), can cause long-lasting deficits in the dopamine (DA) innervation striatum. Although consequences large DA depletions on basal ganglia function have been well characterized, less is known about alterations associated with smaller depletions, those produced by high doses METH. purpose this study was to assess long-term METH-induced depletion function. Three weeks after rats were given multiple administrations METH (5-10 mg/kg, four...

It has been hypothesized that excitatory amino acids can initiate dopamine release in neostriatum. We examined whether the increase extracellular neostriatum produced by acute stress reflects presynaptic initiation of endogenous acids. Thirty minutes intermittent tail-shock significantly elevated concentrations dopamine, glutamate, aspartate, and gamma-aminobutyric acid freely moving rats as measured with vivo microdialysis. Local infusion N-methyl-D-aspartate receptor antagonist...

The aims of this study were: (i) to quantify near-infrared optical properties normal cervical tissues and high-grade squamous intra-epithelial lesions (H-SIL); (ii) assess the feasibility differentiating from H-SIL on basis these properties; (iii) determine how tissue change following photodynamic therapy (PDT) in vivo. Using frequency domain photon migration technique, non-invasive measurements dysplastic ecto-cervical properties, i.e. absorption (μa) effective scattering coefficients,...

Abstract Neurotoxic regimens of methamphetamine ( METH ) result in reactive microglia and astrocytes striatum. Prior data indicate that rats with partial dopamine DA loss resulting from prior exposure to are resistant further decreases striatal when re‐exposed 30 days later. Such animals also do not show an activated phenotype, suggesting a relation between microglial activation ‐induced neurotoxicity. To date, the astrocyte response such resistance has been examined. Thus, this study...

Excitatory amino acid afferents from cerebral cortex and dopamine the substantia nigra synapse on common projection neurons in striatum. Activation of D1 receptors increases immediate early gene expression striatum conductance through N-methyl-d-aspartate (NMDA) receptor. To examine contribution NMDA receptor activation to receptor-mediated responses, we determined effects intrastriatal administration antagonists rotational behavior induced by stimulation rats with unilateral depletions....

N-methyl-d-aspartate receptors (NMDARs) are comprised of different subunits. NR2 subunits confer pharmacological and biophysical properties to NMDARs. Although NR2B subunit expression is uniform throughout striatum, NR2A greater laterally. Pharmacologically isolated NMDAR-mediated excitatory postsynaptic currents (NMDAR-EPSCs) were elicited using minimal local stimulation recorded in the whole cell configuration test hypothesis that NMDAR-EPSCs striatal neurons would vary as a function their...

• Rats become akinetic after large dopamine-depleting brain lesions, yet they show an activation-induced restoration of motor function. In this study, rats were given intraventricular injections the neurotoxin 6-hydroxydopamine to permanently reduce dopamine content corpus striatum by 98%. Although in their home cages, swam effectively when placed deep water and escaped from a shallow floating ice bath. These behaviors not abolished pretreating animals with antagonists haloperidol SCH-23390....

The murine monoclonal antibody (MAb), designated DF3, reacts with a 300-kilodalton (kd) mammary epithelial antigen. A sequential double-determinant enzyme-linked immunoassay (EIA) has been developed to monitor circulating DF3 Previous studies have demonstrated that the use of EIA provides new and potentially useful marker follow clinical course patients metastatic breast cancer. In present study, we monitored antigen in serum ovarian carcinomas non-ovarian gynecologic malignancies....

Abstract The striatum is crucially involved in motor and cognitive function, receives significant glutamate input from the cortex thalamus. corticostriatal pathway arises diverse regions of thought to provide information basal ganglia which actions are selected modified. thalamostriatal specific thalamic nuclei attention possibly strategy switching. Despite these fundamental functional differences, direct comparisons properties pathways lacking. N ‐methyl‐ d ‐aspartate (NMDA) receptors at...

J. Neurochem. (2011) 118 , 668–676. Abstract Neurotoxic doses of methamphetamine (METH) are known to cause depletions in striatal dopamine (DA) tissue content. However, the effects METH‐induced insults on dopaminergic neurotransmission not fully understood. Here, we employed fast‐scan cyclic voltammetry at a carbon‐fiber microelectrode anesthetized rat striatum assess neurotoxic regimen METH phasic and tonic modes signaling underlying mechanisms DA release uptake. Extracellular was...

The aim of this paper was to evaluate the factors that predict regression untreated CIN 2 and 3. A total 93 patients with colposcopic persistent 3 lesions after biopsy were followed for 6 months. Human papillomavirus (HPV) types determined by polymerase chain reaction at enrolment. We analysed biologic demographic predictors natural using univariate multivariate methods. overall rate 52% (48 out 93), including 58% (22 38) 47% (26 55) (P=0.31 difference). detected in 84% (78 93) patients. In...

Methamphetamine (METH) is a highly addictive drug that also neurotoxic to central dopamine (DA) systems. Although striatal DA depletions induced by METH are associated with behavioral and cognitive impairments, the link between these phenomena remains poorly understood. Previous work in both METH-pretreated animals 6-hydroxydopamine model of Parkinson's disease suggests disruption phasic signaling, which important for learning goal-directed behavior, may be such link. However, previous...