ABSTRACT The Asian citrus psyllid, Diaphorina citri , is the natural vector of causal agent Huanglongbing (HLB), or greening disease. Together; HLB and D. represent a major threat to world production. As there no cure for HLB, insect management considered one strategy help control disease, viruses might be useful. In this study, we used metagenomic approach analyze viral sequences associated with global population . By sequencing small RNAs transcriptome coupled bioinformatics analysis,...

Cucumber mosaic virus (CMV) has a worldwide distribution and the widest host range of any known plant virus. From 2000 to 2012, epidemics CMV severely affected production snap bean (Phaseulos vulgaris L.) in Midwest Northeastern United States. Virus diversity leading emergence new strains is often considered significant factor epidemics. In addition epidemics, disease phenotypes arising from genetic exchanges or mutation can compromise effectiveness management strategies. Here, we captured...

Citrus sudden death (CSD) has caused the of approximately four million orange trees in a very important citrus region Brazil. Although its etiology is still not completely clear, symptoms and distribution affected plants indicate viral disease. In search for viruses associated with CSD, we have performed comparative high-throughput sequencing analysis transcriptome small RNAs from CSD-symptomatic -asymptomatic using Illumina platform. The data revealed mixed infections that included tristeza...

Wheat viruses including wheat streak mosaic virus, Triticum and barley yellow dwarf virus cost substantial losses in crop yields every year. Although there have been extensive studies conducted on these known viruses, currently, is limited knowledge about all components of the (

Wheat streak mosaic (WSM), a viral disease affecting cereals and grasses, causes substantial losses in crop yields. virus (WSMV) is the main causal agent of complex, but mixed infections with Triticum (TriMV) High plains wheat emaravirus (HPWMoV) were reported as well. Although resistant varieties are effective for control, WSMV resistance-breaking isolate several potential isolates have been reported, suggesting that populations genetically diverse. Previous phylogenetic studies conducted...

A novel flavi-like virus tentatively named Diaphorina citri (DcFLV) was identified in field populations of through small RNA and transcriptome sequencing followed by reverse transcription (RT)-PCR. We report here the complete nucleotide sequence genome organization DcFLV, largest to date.

ABSTRACT We present here the complete nucleotide sequence and genome organization of a novel putative RNA virus identified in field populations Asian citrus psyllid, Diaphorina citri , through sequencing transcriptome followed by reverse transcription-PCR (RT-PCR). tentatively named this citri-associated C (DcACV). DcACV is an unclassified positive-sense virus.

ABSTRACT Here, we report the complete genome sequence of a putative densovirus Asian citrus psyllid, Diaphorina citri . (DcDNV) was originally identified through metagenomics, and here, obtained nucleotide using PCR-based approaches. Phylogenetic analysis places DcDNV between viruses Ambidensovirus Iteradensovirus genera

Citrus sudden death-associated virus (CSDaV) is a monopartite positive-sense single-stranded RNA that was suggested to be associated with citrus death (CSD) disease in Brazil. Here, we report the first study of genetic structure and molecular variability among 31 CSDaV isolates collected from both symptomatic asymptomatic trees CSD-affected areas. Analyses partial nucleotide sequences five domains genomic RNA, including those encoding for methyltransferase, multi-domain region (MDR),...

Citrus sudden death-associated virus (CSDaV) is a member of the genus Marafivirus in family Tymoviridae, and has been associated with citrus death (CSD) disease Brazil. Difficulties purification CSDaV from infected plants have prevented progress investigation role this CSD an understanding its molecular biology. In work, we constructed full-length cDNA clone driven by 35S promoter (35SRbz-CSDaV). Agrobacterium tumefaciens-mediated inoculation 35SRbz-CSDaV Nicotiana benthamiana enabled fast...

Umbravirus-like associated RNAs (ulaRNAs) are a new group of subviral with plants. Little is known about the biology ulaRNAs. We recently reported wheat umbra-like virus (WULV) from Kansas fields. In this work, we generated full-length cDNA clone WULV which systemically infected N. benthamiana. While agroinfiltrated leaves demonstrated severe necrosis, upper were symptomless. also showed that capable infecting in absence helper virus. Furthermore, and through sap inoculation, transmissible...

Cucumber mosaic virus (CMV) was consistently recovered from symptomatic snap bean plants during surveys conducted in 2007 and 2008 central Wisconsin. A large proportion of these CMV-infected contained a single-stranded linear RNA molecule consisting 339 nucleotides sharing 90-94% sequence identity with other satellite (sat) RNAs CMV. Comparison this satRNA currently available CMV sequences suggests to be novel satRNA.

Plant viruses are emerging and re-emerging to cause important diseases in many plants that humans grow for food and/or fiber, sustainable, effective strategies controlling plant virus remain unavailable [...]

Umbra-like associated RNAs (ulaRNAs) are a new group of subviral with plants. Little is known about the biology ulaRNAs. We recently reported Wheat umbra-like virus (WULV) from Kansas fields. In this work, we generated full-length cDNA clone WULV which efficiently replicated and systemically moved in N. benthamiana. While agroinfiltrated leaves demonstrated severe necrosis, upper were symptomless. also showed that capable autonomous replication wheat without causing symptoms. Furthermore,...