A5083536816- Plant Reproductive Biology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Ubiquitin and proteasome pathways
- Plant Stress Responses and Tolerance
- Protein Kinase Regulation and GTPase Signaling
- Polysaccharides and Plant Cell Walls
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Cellular transport and secretion
- Lipid metabolism and biosynthesis
- Mitochondrial Function and Pathology
- Algal biology and biofuel production
- Research in Cotton Cultivation
- Mass Spectrometry Techniques and Applications
- Photoreceptor and optogenetics research
- Toxin Mechanisms and Immunotoxins
- Advanced Proteomics Techniques and Applications
- Antioxidant Activity and Oxidative Stress
- Legume Nitrogen Fixing Symbiosis
- Enzyme Production and Characterization
- Polyamine Metabolism and Applications
- Light effects on plants
- Plant tissue culture and regeneration
Tel Aviv University
2014-2024
Agricultural Research Organization
2010-2013
Max Planck Institute for Plant Breeding Research
2013
Hebrew University of Jerusalem
1990-2010
Donald Danforth Plant Science Center
2010
University of Zurich
2008
University of Manchester
2008
Swedish University of Agricultural Sciences
2008
University of California, Berkeley
1996-2004
Plant Gene Expression Center
2004
We have assembled a proteome map for Arabidopsis thaliana from high-density, organ-specific catalogs that we generated different organs, developmental stages, and undifferentiated cultured cells. matched 86,456 unique peptides to 13,029 proteins provide expression evidence 57 gene models are not represented in the TAIR7 protein database. Analysis of identified biomarkers allowed us compile an set proteotypic 4105 facilitate targeted quantitative proteomics surveys. Quantitative information...
Protein function is often mediated via formation of stable or transient complexes. Here we report the determination protein-protein interactions in plants using bimolecular fluorescence complementation (BiFC). The yellow fluorescent protein (YFP) was split into two non-overlapping N-terminal (YN) and C-terminal (YC) fragments. Each fragment cloned in-frame to a gene interest, enabling expression fusion proteins. To demonstrate feasibility BiFC plants, pairs interacting proteins were...
Abstract Arabidopsis thaliana calcineurin B–like proteins (CBLs) interact specifically with a group of CBL-interacting protein kinases (CIPKs). CBL/CIPK complexes phosphorylate target at the plasma membrane. Here, we report that dual lipid modification is required for CBL1 function and localization this calcium sensor First, myristoylation targets to endoplasmic reticulum. Second, S-acylation crucial reticulum-to-plasma membrane trafficking via novel cellular targeting pathway insensitive...
Flowering plants produce floral meristems in response to intrinsic and extrinsic flowering inductive signals. In Arabidopsis, the meristem identity genes LEAFY (LFY) APETALA1 (AP1) are activated play a pivotal role specifying during transition. We show here that emerging require AP1 partly specify their identities by directly repressing group of time genes, including SHORT VEGETATIVE PHASE (SVP), AGAMOUS-LIKE 24 (AGL24) SUPPRESSOR OF OVEREXPRESSION CO1 (SOC1). wild-type plants, these...
Rho of plants (ROP) proteins, also known as RAC are Rho-related GTPases that function molecular switches in a multitude signaling cascades involved the regulation actin and microtubule cytoskeleton, vesicle trafficking, plant responses to hormones, stresses, or
Polarized exocytosis is critical for pollen tube growth, but its localization and function are still under debate. The exocyst vesicle-tethering complex functions in polarized exocytosis. Here, we show that a sec3a subunit null mutant cannot be transmitted through the male gametophyte due to defect growth. green fluorescent protein (GFP)-SEC3a fusion functional accumulates at or proximal tip plasma membrane. Partial complementation of resulted development with multiple tips, indicating SEC3...
The RHO proteins, which regulate numerous signaling cascades, undergo prenylation, facilitating their interaction with membranes and proteins called RHO·GDP dissociation inhibitors. It has been suggested that prenylation is required for function. Eleven RHO-related were identified in Arabidopsis. Eight of them are putatively prenylated. We show targeting the remaining three AtRAC7, AtRAC8, AtRAC10, independent, requires palmitoylation, occurs by a cell-specific mechanism. AtRAC8 AtRAC10...
Rho GTPases regulate the actin cytoskeleton, exocytosis, endocytosis, and other signaling cascades. Rhos are subdivided into four subfamilies designated Rho, Racs, Cdc42, a plant-specific group RACs/Rops. This research demonstrates that ectopic expression of constitutive active Arabidopsis RAC, AtRAC10, disrupts cytoskeleton organization membrane cycling. We created transgenic plants expressing either wild-type or AtRAC10 fused to green fluorescent protein. The activated induced deformation...
ROPs or RACs are plant Rho-related GTPases implicated in the regulation of a multitude signaling pathways that function at plasma membrane by virtue posttranslational lipid modifications. The relationship between ROP activation status and localization has not been established. Here we demonstrate endogenous ROPs, as well transgenic His(6)-green fluorescent protein (GFP)-AtROP6 fusion protein, were partitioned Triton X-100-soluble -insoluble membranes. In contrast, an activated...
Development in multicellular organisms depends on the ability of individual cells to coordinate their behavior by means small signaling molecules form correctly patterned tissues. In plants, a unique mechanism directional transport molecule auxin between connects cell polarity and tissue patterning thus is required for many aspects plant development. Direction flow determined polar subcellular localization PIN efflux transporters. Dynamic results from constitutive endocytic cycling plasma...
Abstract Auxin functions as a key morphogen in regulating plant growth and development. Studies on auxin-regulated gene expression the mechanism of polar auxin transport its asymmetric distribution within tissues have provided basis for realizing molecular mechanisms underlying function. In eukaryotes, members Ras Rho subfamilies superfamily small GTPases function switches many signaling cascades that regulate Plants do not proteins, but they contain Rho-like G proteins called RACs or ROPs...
Abstract How plants coordinate developmental processes and environmental stress responses is a pressing question. Here, we show that Arabidopsis (Arabidopsis thaliana) Rho of Plants6 (AtROP6) integrates pathogen response signaling. AtROP6 expression induced by auxin detected in the root meristem, lateral initials, leaf hydathodes. Plants expressing dominant negative (rop6DN) under regulation its endogenous promoter are small have multiple inflorescence stems, twisted leaves, deformed...
The Arabidopsis MADS box transcription factor APETALA1 (AP1) was identified as a substrate for farnesyltransferase and shown to be farnesylated efficiently both in vitro vivo. AP1 regulates the transition from inflorescence shoot floral meristems development of sepals petals. fused green fluorescent protein (GFP) retained activity directed expected terminal flower phenotype when ectopically expressed transgenic Arabidopsis. However, ap1mS, farnesyl cysteine–acceptor mutant AP1, well...
Meristems require a myriad of intercellular signaling pathways for coordination cell division within and between functional zones clonal layers. This control ensures constant availability stem cells throughout the life span meristem while limiting overproliferation meristematic maintaining structure. We have undertaken genetic screen to identify additional components pathways. identified pluripetala ( plp ) mutants based on their dramatically larger meristems increased floral organ number....
Abstract Prenylation primarily by geranylgeranylation is required for membrane attachment and function of type I Rho Plants (ROPs) Gγ proteins, while II ROPs are attached to the plasma S-acylation. Yet, it not known how prenylation affects ROP interaction dynamics what functional redundancy specificity ROPs. Here, we have used expression in mammalian cells together with CaaX prenylation-deficient mutants answer these questions. Our results show that mechanism S-acylation unique plants likely...
Patterning of the root xylem into protoxylem (PX) and metaxylem is regulated by auxin-cytokinin signaling microRNA miR165a/166b-mediated suppression genes encoding Class III HOMEODOMAIN LEU-ZIPPER (HD-ZIPIII) proteins. We found that, in Arabidopsis, osmotic stress via core abscisic acid (ABA) meristematic endodermal cells induces differentiation PX radial longitudinal axes association with increased VND7 expression. Similarly, tomato, ABA enhanced longitudinally radially, indicating an...