A5023786316- HIV Research and Treatment
- Parasitic Infections and Diagnostics
- Amoebic Infections and Treatments
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- HIV/AIDS drug development and treatment
- Infant Nutrition and Health
- Toxoplasma gondii Research Studies
- Veterinary medicine and infectious diseases
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Clostridium difficile and Clostridium perfringens research
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- Parasites and Host Interactions
University of Virginia
2020-2022
University of Maryland, Baltimore County
2015-2016
Howard Hughes Medical Institute
2015-2016
Structural signals that direct HIV packaging During the viral replication cycle of HIV, unspliced dimeric RNA genomes are efficiently packaged into new virions at host cell membrane. Packaging is directed by a region start genome, 5′ leader. The architecture leader remains controversial. Keane et al. developed nuclear magnetic resonance methods to determine structure 155-nucleotide-long can packaging. shows how binds protein directs packaging, selected for and translation suppressed when...
Significance A nucleotide-specific 2 H-edited NMR approach was used to determine the nature of intermolecular interface in intact, dimeric HIV type-1 (HIV-1) 5′-leader RNA (230 kD). The studies distinguish between previously proposed extended duplex and kissing hairpin models identify additional interaction sites. one-dimensional method that allows temporal monitoring base-pair formation revealed observed “extended dimer interface” forms rapidly, even absence chaperones. In addition...
Backgroundgenetic susceptibility to infection is mediated by numerous host factors, including the highly diverse, classical human leukocyte antigen (HLA) genes, which are critical genetic determinants of immunity. We systematically evaluated effect HLA alleles and haplotypes on 12 common enteric infections in children during first year life an urban slum Dhaka, Bangladesh.Methodsa birth cohort 601 Bangladeshi infants was prospectively monitored for diarrhoeal disease. Each stool sample...
Cryptosporidium infection is a leading cause of diarrhea-associated morbidity and mortality in young children globally. Single nucleotide polymorphisms (SNPs) the human protein kinase C-α (PRKCA) gene region have been associated with susceptibility to cryptosporidiosis. Here, we examined role (PKCα) activity HCT-8 intestinal epithelial cells during parvum sporozoites. To delineate PKCα infection, developed fluorescence-based imaging assay differentiate adherent from intracellular parasites....
Human Immunodeficiency Virus Type‐1 (HIV‐1) is responsible for a pandemic that affects roughly 35 million people worldwide. To date, there no cure, and the array of antiretroviral medications serves only to diminish progression disease prevent more rapid deterioration immune system. Many antiretrovirals target four main stages viral life cycle: entry, reverse transcription, integration, maturation. Genome selection packaging, however, relatively less clearly understood, thus current...
Abstract Human Immunodeficiency Virus type-1 (HIV-1) is a retrovirus that responsible for widespread epidemic affecting nearly 36 million individuals worldwide. During the late phase of viral life cycle, Gag polyprotein specifically binds two copies unspliced genomic RNA. consists three structured domains: matrix (MA), capsid (CA), and nucleocapsid (NC). We have shown mature NC to both monomeric dimeric 5’-untranslated region (5’-UTR) RNAs, but fails explain observation genome preferentially...