In recent years, increasing evidence regarding the potential association between periodontal diseases and cardiovascular has been identified. The available underlines importance of detecting pathogens on atheromatous plaque as first step in demonstrating causal relationship two conditions. main aim this investigation is to detect periodontitis-associated bacteria from carotid artery patients who received an endarterectomy using strict sample procurement laboratory procedures.Atheromatous...

Abstract Objective To investigate the effects of an orally administered probiotic on oral microbiota. Methods A placebo‐controlled, parallel study was conducted in 40 gingivitis subjects during 8 weeks. Treatment consisted administration a daily tablet, either containing Lactobacillus reuteri or placebo. Unstimulated saliva and subgingival samples were collected analysed by culture PCR . Clinical microbiological outcome variables compared between within groups. Results There no significant...

Sánchez MC, Llama-Palacios A, Blanc V, León R, Herrera D, Sanz M. Structure, viability and bacterial kinetics of an in vitro biofilm model using six bacteria from the subgingival microbiota. J Periodont Res 2011; 46: 252–260. © 2011 John Wiley & Sons A/S Background Objective: There are few models available scientific literature for study structure, formation development biofilm. The purpose this was to develop validate model, representative selected Material Methods: Six standard reference...

Castillo DM, Sánchez-Beltrán MC, Castellanos JE, Sanz I, Mayorga-Fayad M, Lafaurie GI. Detection of specific periodontal microorganisms from bacteraemia samples after therapy using molecular-based diagnostics. J Clin Periodontol 2011; 38: 418–427. doi: 10.1111/j.1600-051X.2011.01717.x. Abstract Aim: The aim this study was to assess the presence subgingival pathogens in peripheral blood periodontitis patients before and scaling root planing (Sc/RP) nested polymerase chain reaction (nested...

Abstract Objectives To compare biofilm formation on whole dental titanium implants with different surface micro‐topography. Methods A multispecies in vitro model consisting of initial ( Streptococcus oralis and Actinomyces naeslundii ), early Veillonella parvula secondary (Fusobacterium nucleatum ) late colonizers Porphyromonas gingivalis Aggregatibacter actinomycetemcomitans was grown for 96 hr sterile either minimal (S a : 0.5–1.0 mm) or moderate‐roughness surfaces 1.1–2.0 mm). The...

Background and Objective Bacteria in the oral cavity grow form of biofilms; these structures are subject to constant saliva or gingival crevicular fluid flow conditions. The aims this study were: (i) develop characterize an in‐vitro biofilm model with bacteria growing under shear conditions; (ii) demonstrate usefulness for evaluating activity three antiplaque agents. Material Methods We used a bioreactor Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium...

Background and Objectives Differentiation of live dead cells is an important challenge when using molecular diagnosis for microbial identification. This particularly relevant bacteria have been exposed to antimicrobial agents. The objective this study was test a method quantitative real‐time polymerase chain reaction ( qPCR ) combined with propidium monoazide PMA ), developed the selective quantification viable P. gingivalis , A. actinomycetemcomitans F. nucleatum total in vitro biofilm...

Alterations in hormone levels during menopause decrease bone density and may worsen oral health, favoring the growth of periodontal pathogens, whose detection could improve diagnosis periodontitis. The aim this study is to detect quantify main pathogens microbiota postmenopausal females explore relationship between clinical parameters.This was an observational cross-sectional 76 females. Dental examinations sampling for microbiologic evaluation were performed, a history...

Porphyromonas gingivalis, a microorganism residing in the oral cavity within complex multispecies biofilms, is one of keystone pathogens onset and progression periodontitis. In this vitro study, using DNA microarray, we investigate differential gene expression gingivalis ATCC 33277 when growing presence or absence its own monospecies biofilm. Approximately 1.5% genes (28 out 1909 genes, at 1.5 fold change more, p-value < 0.05) were differentially expressed by P. cells These predominantly...

To describe the early biofilm formation over whole dental implants with its micro- and macrostructure, using an in vitro multispecies model.Six bacterial strains (Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans) were used to develop biofilms titanium (growth times 12, 24, 48, 72, 96, 120 hr). The morphology of was studied by confocal laser scanning microscopy electron microscopy,...

Previous research findings support an antimicrobial effect of polyphenols against a variety pathogens, but there is no evidence this periodontal pathogens in complex biofilms. The purpose study was to evaluate the activity red wine and oenological extracts, rich polyphenols, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans Fusobacterium nucleatum total bacteria growing vitro oral biofilm static model.

In search for natural products with antimicrobial properties use in the prevention and treatment of periodontitis, purpose this investigation was to evaluate activity two omega-3 fatty acids, docosahexaenoic acid (DHA) eicosapentaenoic (EPA), using an vitro multi-species subgingival biofilm model including Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans. The activities EPA DHA extracts...

The worrying rise in antibiotic resistances emphasizes the need to seek new approaches for treating and preventing periodontal diseases. purpose of this study was evaluate antibacterial anti-biofilm activity cranberry a validated vitro biofilm model. After chemical characterization selected phenolic-rich extract, its values minimum inhibitory concentration bactericidal were calculated six bacteria forming (

The ecological dysbiosis of a biofilm includes not only bacterial changes but also in their metabolism. Related to oral biofilms, metabolic activity are crucial endpoint, linked directly the pathogenicity diseases. Despite advances caries research, detailed microbial and metabolomic etiology is yet be fully clarified. To advance this knowledge, meta-taxonomic approach based on 16S rRNA gene sequencing an untargeted ultra-high performance liquid chromatography-quadrupole time-of-flight mass...

A bacterial strain capable of growing on propachlor (2-chloro-N-isopropylacetanilide) was isolated from soil by using enrichment and isolation techniques. The isolated, designated GCH1, classified as a member the genus Pseudomonas. Washed-cell suspensions GCH1 accumulated N-isopropylacetanilide, acetanilide, acetamide, catechol. Pseudomonas grew with generation time 4.2 h rate substrate utilization 1.75 +/- 0.15 micromol h(-1). Gene expression did not require induction but subject to...

Background and Objectives One of the major disadvantages DNA ‐based microbial diagnostics is their inability to differentiate between viable dead microorganisms, which could be important when studying etiologically relevant pathogens. The aim this investigation was optimize a method for selective detection quantification only A ggregatibacter actinomycetemcomitans P orphyromonas gingivalis cells by combining quantitative real‐time polymerase chain reaction (q PCR ) propidium monoazide ( PMA...

Summary We have investigated the diversity for composition in seven eggplant ( S olanum melongena ) cultivars of O ccidental type. The results show that, with exception moisture content and p H , there is a wide all analysed traits. Protein was variable, but generally low. available carbohydrates ranged between 2.99 4.19 mg 100 g −1 main soluble sugars were glucose fructose. fibre most variable trait. In cases, dehydroascorbic acid higher than ascorbic content. Total phenolics on average...

Aggregatibacter actinomycetemcomitans (Aa) is a pathogenic bacterium residing in the subgingival plaque biofilm strongly associated with pathogenesis of periodontitis. The aim this investigation was to study protein differential expression Aa when growing on compared planktonic state using proteomic analysis by 2D-DIGE system. Eighty-seven proteins were differentially expressed during growth (1.5-fold, p < 0.05), 13 overexpressed and 37 down-expressed. Those repressed mainly involved...