A5114377778- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Fungal Biology and Applications
- Marine Sponges and Natural Products
- Synthetic Organic Chemistry Methods
- Bioactive Compounds and Antitumor Agents
- Plant Pathogens and Fungal Diseases
- Plant biochemistry and biosynthesis
- Traditional and Medicinal Uses of Annonaceae
- Cancer therapeutics and mechanisms
- Microbial Metabolism and Applications
- Polysaccharides and Plant Cell Walls
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Phytochemical compounds biological activities
- Food Industry and Aquatic Biology
- CRISPR and Genetic Engineering
- Carbohydrate Chemistry and Synthesis
- Synthesis and Biological Activity
- Bioactive Natural Diterpenoids Research
- Phytochemicals and Antioxidant Activities
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Biological Activity of Diterpenoids and Biflavonoids
- Ubiquitin and proteasome pathways
Agency for Science, Technology and Research
2018-2025
Star Technology and Research (United States)
2025
Bioinformatics Institute
2018-2020
Singapore Science Park
2000-2015
National University of Singapore
1990-2007
MerLion Pharma (Germany)
2004
Institute of Molecular and Cell Biology
1990-2002
National Institute of Water and Atmospheric Research
2002
University of Mississippi
2002
Abstract Natural products possess significant therapeutic potential but remain underutilized despite advances in genomics and bioinformatics. While there are approaches to activate upregulate natural product biosynthesis both native heterologous microbial strains, a comprehensive strategy elicit production of as well generalizable efficient method interrogate diverse strains collection, remains lacking. Here, we explore flexible robust integrase-mediated multi-pronged activation approach...
High resolution two-dimensional gel electrophoresis was used to analyze the signal transduction pathways of tumor necrosis factor (TNF-alpha) and interleukin 1 (IL-1 alpha -beta) in human fibroblasts. Approximately 450 discrete radioactive spots were electrophoretically resolved from cytosolic extracts cells prelabeled with 32P. At least 63 these polypeptides exhibited significant concordant phosphorylation or dephosphorylation response TNF IL-1, despite fact that different receptors are...
Two novel human cytomegalovirus protease inhibitors, cytonic acids A (1) and B (2), have been isolated from the solid-state fermentation of endophytic fungi Cytonaema sp. Their structures as p-tridepside isomers were elucidated by MS NMR methods.
Isoforms of heat shock protein (Hsp) 27 were used as intracellular markers to study tumor necrosis factor/interleukin-1 (TNF/IL-1) regulation phosphatases in primary human fibroblasts. These isoforms rapidly phosphorylated varying degrees when fibroblasts treated with either TNF, IL-1, okadaic acid, calyculin A, ARS, epidermal growth factor, fibroblast H2O2, buthionine sulfoximine, N-ethylmaleimide, diethylmaleimide, or iodoacetate. However, inhibitors kinases A and C, tyrosyl kinases,...
In addition to the sesquiterpene-phenol aureols (1), 6'-chloroaureol (2), and aureol acetate (3), eight indole alkaloids including new N-3'-ethylaplysinopsin (9) have been isolated from Jamaican sponge Smenospongia aurea. Makaluvamine O (10), a member of pyrroloiminoquinone class, was also isolated. The structures were characterized by spectroscopic methods, two derivatives prepared optimize biological activity. Aureol N,N-dimethyl thiocarbamate (1a) 6-bromoaplysinopsin (7) exhibit...
The crude extract of the broth Aspergillus ochraceus was found to inhibit final stage polyprotein processing during hepatitis C virus replication. Bioassay-guided fractionation led isolation known compound mellein as active component extract. Also isolated were circumdatin F and a new alkaloid, G. structure G determined by spectroscopic analysis.
Although tumor necrosis factor (TNF) and interleukin-1 (IL) induce the expression of IL-8, TNF-stimulated gene 6, plasminogen activation inhibitor-2 genes NF-kappa B with nearly identical kinetics, two cytokines differ significantly in induction IL-6 all primary fibroblasts tested. IL-1 induces after 30 min treatment, reaching a maximum level by 7 h, is sustained for up to 14 h. TNF also at but was low. A similar differences amount secreted observed IL-1/TNF-treated cells. Measurement...
Sortase A (SrtA) is a membrane-associated enzyme that anchors surface-exposed proteins to the cell wall envelope of Gram-positive bacteria such as Staphylococcus aureus. As SrtA essential for bacterial pathogenesis but dispensable microbial growth or viability, considered favorable target enhancement novel anti-infective drugs aim interfere with key virulence mechanisms, biofilm formation, without developing drug resistance. Here, we used virtual screening search an in-house natural compound...
In recent years, CRISPR-Cas toolboxes for Streptomyces editing have rapidly accelerated natural product discovery and engineering. However, Cas efficiencies are oftentimes strain-dependent, the commonly used Streptococcus pyogenes Cas9 (SpCas9) is notorious having high levels of off-target toxicity effects. Thus, a variety proteins required greater flexibility genetic manipulation within wider range strains. This study explored first use Acidaminococcus sp. Cas12j, hypercompact Cas12...
Two new compounds, 10-methoxydihydrofuscin (1) and fuscinarin (2), one known compound, fuscin (3), have been isolated from the soil fungus Oidiodendron griseum. These compounds were found to compete effectively with macrophage inflammatory protein (MIP)-1α for binding human CCR5, an important anti HIV-1 target that interferes HIV entry into cells. The structures of these elucidated by spectroscopic methods.
Endophytic microorganisms are an important source of bioactive secondary metabolites. In this study, fungal endophytes obtained from A*STAR's Natural Product Library (NPL) and previously isolated different habitats Singapore were investigated for their diversity, antimicrobial, cytotoxic activities. A total 222 strains identified on the basis sequence analysis ITS region rDNA gene. The belong to 59 genera distributed in 20 orders. Majority (99%; 219 strains) phylum Ascomycota, while two...
Nature has provided unique molecular scaffolds for applications including therapeutics, agriculture, and food. Due to differences in ecological environments laboratory conditions, engineering is often necessary uncover utilize the chemical diversity. Although we can efficiently activate mine these complex 3D molecules, sufficient production of target molecules further application remain a considerable bottleneck. An example bioactive armeniaspirols, which are potent polyketide antibiotics...
Researchers are increasingly interested in discovering new pancreatic lipase inhibitors as anti-obesity ingredients. Medicine-and-food homology plants contain a diverse set of natural bioactive compounds with promising development potential. This study screened and identified potent from 20 commonly consumed medicine-and-food using affinity ultrafiltration combined spectroscopy docking simulations. The results showed that turmeric exhibited the highest lipase-inhibitory activity, curcumin,...
A prefractionated Streptomyces-derived extract was initially identified as being active using a luciferase-based AMP-activated protein kinase (AMPK) assay. Bioassay-guided fractionation led to the isolation of new compound quinazolin-4(3H)-one (1) component. However, 1 shown have potent firefly luciferase inhibitory activity with no effect on AMPK. This is first report natural inhibitor.