A5043098072- CRISPR and Genetic Engineering
- Pleural and Pulmonary Diseases
- RNA Interference and Gene Delivery
- Amyotrophic Lateral Sclerosis Research
- Innovation and Socioeconomic Development
- Occupational and environmental lung diseases
- Advanced biosensing and bioanalysis techniques
- Neurogenetic and Muscular Disorders Research
- Pluripotent Stem Cells Research
- Lung Cancer Diagnosis and Treatment
- Identification and Quantification in Food
- Neurobiology and Insect Physiology Research
- Ovarian cancer diagnosis and treatment
- Colorectal Cancer Surgical Treatments
- Fish Ecology and Management Studies
- Advancements in Transdermal Drug Delivery
- Autophagy in Disease and Therapy
- Photochemistry and Electron Transfer Studies
- Nanoparticle-Based Drug Delivery
- Fish Biology and Ecology Studies
- Prion Diseases and Protein Misfolding
- Pituitary Gland Disorders and Treatments
- Photosynthetic Processes and Mechanisms
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Insect and Pesticide Research
The University of Sydney
2020-2025
Royal North Shore Hospital
2021-2025
Macquarie University
2012-2024
Garvan Institute of Medical Research
2011-2020
Health Sciences Centre
2019
Pain is a significant medical concern and represents major unmet clinical need. The ability to perceive react tissue-damaging stimuli essential in order maintain bodily integrity the face of environmental danger. To prevent damage systems that detect noxious are therefore under strict evolutionary pressure. We developed high-throughput behavioral method identify genes contributing thermal nociception fruit fly have reported large-scale screen identified Ca2+ channel straightjacket (stj) as...
Abstract Precise genome editing is limited by the inefficiency of homology-directed repair (HDR) compared to non-homologous end-joining (NHEJ) double strand breaks (DSBs). The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 system generates precise, locus-specific DSBs that can serve as substrates for HDR. We developed an in vivo visual reporter assay quantify HDR-mediated events at single-cell resolution zebrafish and used this identify small-molecule modulators shift...
The CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile efficient introduction of heritable locus-specific sequence modifications in the genome. Despite its molecular precision, temporal spatial control gene editing with system is very limited. We developed light-sensitive liposome delivery that offers high degree system. demonstrated protein release by respectively assessing targeted knockout eGFP human HEK293/GFP cells TNFAIP3 TNFα-induced HEK293 cells....
Aims Recently, there have been attempts to improve prognostication and therefore better guide treatment for patients with medullary thyroid carcinoma (MTC). In 2022, the International MTC Grading System (IMTCGS) was developed validated using a multi‐institutional cohort of 327 patients. The aim current study build upon findings IMTCGS develop validate prognostic nomogram predict recurrence‐free survival (RFS) in MTC. Methods Results Data from 300 five centres across USA, Europe, Australia...
Abstract Human genetic disorders, arising from a range of irregularities, can significantly affect human physiology, often with limited available treatment options. The development the CRISPR system, facilitating precise editing genome, has opened new avenues for addressing mutations found in various disorders. However, there is currently lack comprehensive reviews that specifically address application diseases. To bridge this gap, review focuses on exploring advancements technology and...
Abstract Background There is uncertainty when determining the optimal treatment for malignant polyps. Clinicians must balance oncological risk of a polyp with morbidity and mortality from surgery. This study developed an online risk‐calculator using machine‐learning techniques to predict adverse outcome colorectal following polypectomy. Methods Retrospective data collection population‐wide database all polyps 2011 2020 was performed. Utilizing artificial intelligence‐based machine learning...
Although there is early support for schemes based on nuclear grade, necrosis and mitotic rate, currently no widely implemented grading system diffuse pleural mesothelioma (DPM). We investigated current systems propose a novel Mesothelioma Weighted Grading Scheme (MWGS). The MWGS assigns weighted scores from 0 to 10 age (≤74, >74 yrs: 0,1); histologic type (epithelioid, biphasic, sarcomatoid: 0,1,2); (absent, present: 0,2); count per 2 mm2 (≤1, 4, ≥5: atypia (mild, moderate, severe:...
The Lake Eacham rainbowfish (Melanotaenia eachamensis) was once thought to be confined its type locality within the World Heritage National Park. M. eachamensis disappeared from lake following translocation of several species into and pronounced extinct in wild 1987. In a 2007 survey we noticed that were present again. We used molecular marker identify these fish likely source population. Analysis D-loop region mitochondrial DNA revealed now is Melanotaenia splendida, most closely related...
Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease characterized by the degeneration of motor neurons. Mutations in cyclin F (CCNF) and fused sarcoma (FUS) genes have been associated with ALS pathology. In this study, we aimed to investigate functional role CCNF FUS using genome editing techniques generate zebrafish models genetic disruptions these genes. Sequence comparisons showed significant homology between human proteins. We used CRISPR/Cas9 TALEN-mediated...
ABSTRACT The CRISPR-Cas9 and related systems offer a unique genome editing tool allowing facile efficient introduction of heritable locus-specific sequence modifications in the genome. Despite its molecular precision, temporal spatial control gene with system is very limited. We developed light-sensitive liposome delivery that offers high degree CRISPR/Cas9 system. demonstrated transfection efficiency, by assessing targeted knockout eGFP human HEK293 cells (52.8% knockout). further validated...