A5054791447- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Photodynamic Therapy Research Studies
- Nanoparticle-Based Drug Delivery
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Biosensors and Analytical Detection
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Biosensing Techniques and Applications
- Radiation Therapy and Dosimetry
- Luminescence and Fluorescent Materials
- Advanced Nanomaterials in Catalysis
- Recycling and utilization of industrial and municipal waste in materials production
- Viral Infectious Diseases and Gene Expression in Insects
- Luminescence Properties of Advanced Materials
- Nanocluster Synthesis and Applications
- Concrete and Cement Materials Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Innovation and Socioeconomic Development
- Bone Tissue Engineering Materials
- RNA modifications and cancer
- Evolutionary Algorithms and Applications
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Photoacoustic and Ultrasonic Imaging
UNSW Sydney
2018-2025
University of Technology Sydney
2022-2025
Northwestern Polytechnical University
2024
University of Science and Technology Beijing
2011-2024
Lanzhou University
2021-2023
China Medical University
2023
First Affiliated Hospital of Gannan Medical University
2012-2022
Nanjing Medical University
2022
Southwestern University of Finance and Economics
2022
First Hospital of Qinhuangdao
2021
Liposomes have been well established as an effective drug delivery system, due to simplicity of their preparation and unique characteristics. However conventional liposomes are unsuitable for the on-demand content release, which limits therapeutic utility. Here we report X-ray-triggerable incorporating gold nanoparticles photosensitizer verteporfin. The 6 MeV X-ray radiation induces verteporfin produce singlet oxygen, destabilises liposomal membrane causes release cargos from cavity. This...
Abstract Control of CRISPR/Cas12a trans -cleavage is crucial for biosensor development. Here, we show that small circular DNA nanostructures which partially match guide RNA sequences only minimally activate Cas12a ribonucleoproteins. However, linearizing these structures restores activation. Building on this finding, an Autocatalytic Circular Amplification Reaction (AutoCAR) system established allows a single nucleic acid target to multiple ribonucleoproteins, and greatly increases the...
Abstract Singlet oxygen is a primary cytotoxic agent in photodynamic therapy. We show that CeF 3 nanoparticles, pure as well conjugated through electrostatic interaction with the photosensitizer verteporfin, are able to generate singlet result of UV light and 8 keV X-ray irradiation. The stimulated quantum yield was determined be 0.79 ± 0.05 for conjugate 31 verteporfin molecules per nanoparticle, highest conjugation level used. From this we estimate dose generated from -verteporfin...
The development of vaccines against specific types cancers will offer new modalities for therapeutic intervention. Here, we describe the synthesis a novel vaccine construction prepared from spherical gold nanoparticles 3–5 nm core diameters. particles were coated with both tumor-associated glycopeptides antigens containing cell-surface mucin MUC4 Thomsen Friedenreich (TF) antigen attached at different sites and 28-residue peptide complement derived protein C3d to act as B-cell activating...
In this work, we brought together two existing clinical techniques used in cancer treatment—X-ray radiation and photodynamic therapy (PDT), whose combination termed X-PDT uniquely allows PDT to be therapeutically effective deep tissue. To end, developed mitochondrially targeted biodegradable polymer poly(lactic-co-glycolic acid) nanocarriers incorporating a photosensitizer verteporfin, ultrasmall (2–5 nm) gold nanoparticles as enhancers, triphenylphosphonium acting the mitochondrial...
Upconversion (UC) fluorescence in NaYF4:Yb, Er nanoparticles amplified by metal nanostructures was compared two nanostructure geometries: gold nanoshells surrounding and silver adjacent to the nanoparticles, both placed on a dielectric silica surface. Enhanced UC luminescence signals modified lifetimes induced these metals were observed our study. The intensities of green red emissions enhanced Ag factor approximately 4.4 3.5, respectively. corresponding reduced ∼ 1.7-fold 2.4-fold. In...
Eu chelate-doped plasmonic silver-core-silica-shell nanocomposites show greatly increased fluorescence enhancement factors of up to 145 at high excitation intensities, due significantly radiative rates in samples with metal cores. They offer exceptionally signal intensity, sufficient for single particle detection and compatibility time-gated imaging offering nearly background-free conditions. A bioassay application is also presented. Detailed facts importance specialist readers are published...
Abstract Time-gated luminescence microscopy using long-lifetime molecular probes can effectively eliminate autofluorescence to enable high contrast imaging. Here we investigate a new strategy of time-gated imaging for simultaneous visualisation multiple species microorganisms stained with long-lived complexes under low-background conditions. This is realized by two pathogenic organisms ( Giardia lamblia red europium probe and Cryptosporidium parvum green terbium probe) at UV wavelengths...
Introduction: The depth limitation of conventional photodynamic therapy (PDT) with visible electromagnetic radiation represents a challenge for the treatment deep-seated tumors. Materials and methods: To overcome this issue, we developed an X-ray-induced PDT system where poly(lactide- co -glycolide) (PLGA) polymeric nanoparticles (NPs) incorporating photosensitizer (PS), verteporfin (VP), were triggered by 6 MeV X-ray to generate cytotoxic singlet oxygen. used in study allows breakthrough...
The CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile efficient introduction of heritable locus-specific sequence modifications in the genome. Despite its molecular precision, temporal spatial control gene editing with system is very limited. We developed light-sensitive liposome delivery that offers high degree system. demonstrated protein release by respectively assessing targeted knockout eGFP human HEK293/GFP cells TNFAIP3 TNFα-induced HEK293 cells....
Abstract In this study, we constructed multifunctional liposomes with preferentially mitochondria-targeted feature and gold nanoparticles-assisted synergistic photodynamic therapy. We systemically investigated the in vitro X-ray triggered PDT effect of these on HCT 116 cells including levels singlet oxygen, mitochondrial membrane potential, cell apoptosis/necrosis expression apoptosis-related proteins. The results corroborated that synchronous action radiation enhance generation cytotoxic...
Current chemo/biosensors for hypochlorous acid or hypochlorite detections are usually limited to the submicromolar level because of their insufficient sensitivity, which is a problem concentrations in biological matrices generally on nanomolar scale even lower. Developing probe with high enough sensitivity remains challenge. Using minimal background fluorescence upconversion nanocrystals our advantage, we herein report an energy-transfer mechanism-based luminescent nanosensor sensitive and...
Long non-coding RNA MEG3 is suggested to function as a tumor suppressor. However, the activation mechanism of still not well understood and data are available on its role under adenosine-induced apoptosis. In this study, HepG2 cells were treated with adenosine or 5-Aza‑cdR. Methylation status promoter was detected by methylation specific PCR (MSP) expression determined qRT-PCR. PcDNA3.1-MEG3 recombinant plasmid constructed transfected hepatoma Huh7 cells. Cell growth, morphological changes,...