A5086684693- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Osteoarthritis Treatment and Mechanisms
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Bone and Dental Protein Studies
- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Oral microbiology and periodontitis research
- Dental Trauma and Treatments
- Metal complexes synthesis and properties
- Salivary Gland Disorders and Functions
- Inorganic and Organometallic Chemistry
- Marine Invertebrate Physiology and Ecology
- Dental Research and COVID-19
- Cell death mechanisms and regulation
- Osteomyelitis and Bone Disorders Research
- Gout, Hyperuricemia, Uric Acid
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- Ginseng Biological Effects and Applications
Kunming Medical University
2017-2025
Stomatology Hospital
2019-2025
Sichuan University
2010-2021
State Key Laboratory of Oral Diseases
2010-2021
Abstract Background Treatments based on stem cell-derived small extracellular vesicles (sEVs) have been explored as an alternative to cell transplantation-based therapies in periodontal regeneration. Dental follicle cells (DFSCs) shown great potential for regenerative medicine applications. However, it is unclear whether sEVs derived from DFSCs (DFSCs-sEVs) could be used This study investigates DFSCs-sEVs regenerate damaged tissue and the underlying mechanism. Methods were isolated...
Abstract Objectives The function of the dental pulp is closely connected to extracellular matrix ( ECM ) structure, and has received significant attention due its biological functions for regulating cells. As such, interaction between niche cells worth exploring potential clinical uses. Materials methods In this study, stem cell DPSC )‐derived DPM was prepared through culture decellularization as niche, changes in behaviour histological analysis tissue regeneration were evaluated following...
Temporomandibular joint osteoarthritis (TMJ-OA), mainly exhibit extracellular matrix loss and condylar cartilage degradation, is the most common chronic degenerative maxillofacial osteoarthritis; however, no efficient therapy for TMJ-OA exists due to poor understanding of its pathological progression. MicroRNA (miR)-140-5p a novel non-coding microRNAs (miRNAs) that expressed in specifically. To investigate molecular mechanisms miR-140-5p TMJ-OA, primary mandibular chondrocytes (MCCs) from...
Abstract Aim To identify an optimized strategy for the large‐scale production of nanovesicles (NVs) that preserve biological properties exosomes (EXOs) use in periodontal regeneration. Materials and Methods NVs from dental follicle stem cells (DFSCs) were prepared through extrusion, EXOs DFSCs isolated. The yield both extruded (eNVs) quantified protein concentration particle number analyses. Their pro‐migration, pro‐proliferation pro‐osteogenesis capacities compared subsequently vitro....
Periodontitis (PD) is a common chronic inflammatory oral disease that severely affects patients' quality of life. Fisetin has been shown to possess antioxidant and anti-inflammatory properties in various biological systems.
The mechanism of temporomandibular joint osteoarthritis (TMJOA), which leads to the final erosion cartilage and subchondral bone, has been widely demonstrated, but still not clearly elucidated. Many studies have pointed that NLRP3-mediated inflammation played a vital role in degenerative diseases. However, its interaction with synovitis TMJOA remained poorly investigated. In our study, we explored NLRP3 inflammasome therapeutic potential caspase-1 inhibitors. By establishing rat model, found...
Tissue or organ regeneration using xenogeneic matrices is a promising approach to address the shortage of donor for allotransplantation. Success such has been demonstrated correlate with macrophage-mediated fibrotic homeostasis and tissue remodeling. The previous studies have that treated dentin matrix (TDM) could be suitable bioactive substrate allogeneic tooth root regeneration. This study constructed bioengineered (bio-root) via combination porcine TDM (pTDM) dental follicle cells (DFCs)....
Successful regenerative medicine strategies of xenogeneic extracellular matrix need a synergistic balance among inflammation, fibrosis, and remodeling process. Adaptive macrophage subsets have been identified to modulate inflammation orchestrate the repair neighboring parenchymal tissues. This study fabricated PPARγ-primed CD68+CD206+ M2 phenotype (M2γ), firstly verified their anti-inflammatory tissue-regenerating roles in bioengineered organ regeneration. Our results showed that Th1-type...
Lipopolysaccharide exerts many effects on cell lines, including cytokine secretion, and apoptosis necrosis. We investigated the in vitro of lipopolysaccharide cultured human dental pulp cells expression Bcl-2 Bax. Dental showed morphologies typical after exposure to lipopolysaccharide. Flow cytometry that rate increased with increasing concentration. Compared controls, promoted (P < 0.05) from 0.1 100 μg/mL but not at 0.01 μg/mL. Cell was statistically higher for 3 days compared 1 day, no...
Extracellularmatrix (ECM)‐based scaffolds are important for their potential therapeutic application. Treated dentin matrix (TDM), a kind of ECM, seeded with allogeneic dental follicle stem cells (TDM/aDFC) provides suitable inductive microenvironment tooth root regeneration. Considering the limited sources, xenogeneic TDM (xTDM) is possible alternative to TDM; however, xTDM‐based scaffold presents severe osteolysis and resorption lacunae causing regenerated failure. Immune response–induced...
Abstract Commonly recognized mechanisms of the xenogeneic-extracellular matrix-based regenerative medicine include timely degradation, release bioactive molecules, induced differentiation stem cells, and well-controlled inflammation. This process is most feasible for stromal tissue reconstruction, yet unsuitable non-degradable scaffold prefabricated-shaped regeneration, like odontogenesis. Treated dentin matrix (TDM) has been identified as a regeneration. study explored xenogeneic porcine...
Abstract Aim To compare the efficacy of adipocyte‐derived dedifferentiated fat (DFAT) cell and adipose‐derived stromal (ADSC) sheets for regenerative treatment intra‐bony periodontal defects. Materials Methods DFAT cells were obtained using ceiling culture method compared with ADSCs Cell Counting Kit‐8, colony formation assay, surface antigen identification, multilineage differentiation assays. ADSC prepared in a sheet medium. The biological characteristics haematoxylin eosin staining,...
Extracellular matrix (ECM)-based biomaterials developed from mammalian tissues have been successfully used in preclinical and clinical tissue engineering applications. We previously reported about the applicability of dentin-based scaffold, treated dentin (TDM), for tooth root regeneration. However, TDM protein composition has not characterized. Here, we a shotgun proteomic strategy to profile human proteome. N-glycoproteins were enriched by lectin affinity chromatography identified mass...
Abstract Background It remains unclear etiology of cartilaginous tissues in osteoarthritis (OA) lesions. In this study, we hypothesized the accumulation hypoxia-inducible factor (HIF) and activated apoptosis relate to condylar cartilage degeneration vivo. Methods Malocclusion stress was applied for 2 weeks, 4 weeks 8 induce an OA-like lesion animal model rats. Histological analysis performed by H&E staining Safranin O/fast green staining. The expression levels protein were examined...
This study aims to explore the role of exosomes from cancer-associated fibroblasts (CAFs) induced by PDGF-BB in promoting malignancy oral squamous cell carcinoma (OSCC) and provide new insight into mechanism OSCC progression its treatment.Exosomes were extracted human mucosa (hOMFs) CAFs. Differentially expressed miRNAs between hOMFs CAFs analysed using high-throughput sequencing self-programmed R software. Cal-27, a tongue line, was treated with exosomes. clinical cancer tissues adjacent...
<b><i>Introduction:</i></b> Osteoarthritis (OA) is a common musculoskeletal disease characterized by pain, stiffness, limited activity, occasional effusion, and local inflammation. MiR-146 one of the noncoding RNA closely related to OA, but role miR-146 in OA remains controversial. The tumour necrosis factor receptor OX40 activated its cognate ligand OX40L (TNFSF4) functions as T-cell costimulatory molecule. functions, including cytokine production, expansion,...
Periodontitis is an inflammatory, infectious disease of polymicrobial origin that can damage tooth-supporting bone and tissue. Tree shrews, evolutionarily closer to humans than commonly used rodent models, have been increasingly as biomedical models. However, a tree shrew periodontitis model has not yet established.Periodontitis was induced in male shrews/Sprague-Dawley rats by nylon thread ligature placement around the lower first molars. Thereafter, morphometric histological analyses were...
Abstract Aim To investigate the cell status of dental pulp cells ( DPC s) in a sphingosine‐1‐phosphate S 1 P )‐induced microinflammation environment and possible mechanisms homoeostasis maintenance by . Methodology Sphingosine‐1‐phosphate receptor (S1 PR ) expression was examined s within local S1P‐induced model established using μmol L −1 U 0126 [extracellular signal‐regulated kinase ERK inhibitor], LY 294002 AKT inhibitor) Y 27632 ROCK were used to inhibit corresponding signalling pathways...