A5037945408- HER2/EGFR in Cancer Research
- Monoclonal and Polyclonal Antibodies Research
- Advanced Breast Cancer Therapies
- BRCA gene mutations in cancer
- PARP inhibition in cancer therapy
- Breast Cancer Treatment Studies
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Estrogen and related hormone effects
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- Advanced Biosensing Techniques and Applications
- Inflammatory mediators and NSAID effects
- Melanoma and MAPK Pathways
- Cancer Treatment and Pharmacology
- Phagocytosis and Immune Regulation
- Prostate Cancer Treatment and Research
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Immune cells in cancer
- Radiopharmaceutical Chemistry and Applications
- Cytokine Signaling Pathways and Interactions
- Viral Infections and Outbreaks Research
- Hearing, Cochlea, Tinnitus, Genetics
Vanderbilt University
2013-2025
The University of Texas Medical Branch at Galveston
2013-2025
Vanderbilt University Medical Center
2013-2023
Breast Cancer Research Foundation
2023
Vanderbilt-Ingram Cancer Center
2016
Istituto Nazionale Tumori IRCCS "Fondazione G. Pascale"
2016
University College London
2015
Institute of Cancer Research
2015
Cincinnati Children's Hospital Medical Center
2006-2013
Duquesne University
2013
After an initial response to chemotherapy, many patients with triple-negative breast cancer (TNBC) have recurrence of drug-resistant metastatic disease. Studies TNBC cells suggest that chemotherapy-resistant populations stem-like (CSCs) self-renewing and tumor-initiating capacities are responsible for these relapses. TGF-β has been shown increase properties in human cells. We analyzed RNA expression matched pairs primary biopsies before after chemotherapy. Biopsies chemotherapy displayed...
MerTK, a receptor tyrosine kinase (RTK) of the TYRO3/AXL/MerTK family, is expressed in myeloid lineage cells which it acts to suppress proinflammatory cytokines following ingestion apoptotic material. Using syngeneic mouse models breast cancer, melanoma, and colon we found that tumors grew slowly were poorly metastatic MerTK-/- mice. Transplantation bone marrow, but not wild-type into lethally irradiated MMTV-PyVmT mice (a model cancer) decreased tumor growth altered cytokine production by...
Deficiencies in DNA double-strand break (DSB) repair lead to genetic instability, a recognized cause of cancer initiation and evolution. We report that the retinoblastoma tumor suppressor protein (RB1) is required for DSB by canonical non-homologous end-joining (cNHEJ). Support cNHEJ involves mechanism independent RB1's cell-cycle function depends on its amino terminal domain with which it binds NHEJ components XRCC5 XRCC6. Cells engineered loss RB family as well cancer-derived cells...
Approximately one-third of Lassa virus (LASV)-infected patients develop sensorineural hearing loss (SNHL) in the late stages acute disease or early convalescence. With 500,000 annual cases fever (LF), LASV is a major cause regions West Africa where LF endemic. To date, no animal models exist that depict human pathology with associated loss. Here, we aimed to an model study LASV-induced using isolates from 2012 Sierra Leone outbreak. We have recently established murine for closely mimics many...
Immunotherapies targeting programmed cell death protein 1 (PD-1) or its ligand, ligand (PD-L1), dramatically improve the survival of melanoma patients. However, only ∼40% treated patients demonstrate a clinical response to single-agent anti-PD-1 therapy. An intact tumor type-II interferon (i.e. IFN-γ) correlates with anti-PD-1, and de novo acquired resistance may harbor loss-of-function alterations in JAK/STAT pathway, which lies downstream gamma receptor (IFNGR1/2). In this study, we...
Olfactory dysfunction is one of the most frequent and specific symptoms coronavirus disease 2019 (COVID-19). Information on damage repair neuroepithelium its impact olfactory function after COVID-19 still incomplete. While severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes ongoing worldwide outbreak COVID-19, little known about changes triggered by SARS-CoV-2 in epithelium (OE) at cellular level. Here, we report profiles OE infection golden Syrian hamsters, which a reliable...
With altered sense of taste being a common symptom coronavirus disease 2019 (COVID-19), the main objective was to investigate presence and distribution severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) within tongue over course infection. Golden Syrian hamsters were inoculated intranasally with SARS-CoV-2 tongues collected at 2, 3, 5, 8, 17, 21, 35, 42 days post-infection (dpi) for analysis. In order test gross changes in tongue, papillae counted. Paraffin-embedded thin sections...
We report the coencapsulation of fluorocoxib Q (FQ) and chemocoxib A (CA) in micellar nanoparticles (FQ-CA-NPs) a new PPS135-b-POEGA17 diblock polymer, which exhibited hydrodynamic diameter 109.2 ± 4.1 nm zeta potential (ζ) -1.59 0.3 mV. The uptake FQ-CA-NPs by 4T1 mouse mammary cancer cells intracellular cargo release were assessed fluorescence microscopy that resulted increased compared to pretreated with celecoxib. viability primary human epithelial (HMECs) or carcinoma treated assessed,...
Carriers of germ line mutations in breast cancer susceptibility gene BRCA1 have an increased risk developing and ovarian cancers; missense have, however, been difficult to assess for disease association. Here we used a biophysical approach classify these variants. We established assay measuring the thermodynamic stability BRCT domains investigated effects 36 mutations. The show range effects. Some do not change stability, whereas others destabilize protein by as much 6 kcal mol(-1);...
Triple-negative breast cancers (TNBCs) are heterogeneous and aggressive, with high mortality rates. TNBCs frequently respond to chemotherapy, yet many patients develop chemoresistance. The molecular basis roles for tumor cell–stromal crosstalk in establishing chemoresistance complex largely unclear. Here we report studies of paired TNBC patient–derived xenografts (PDXs) established before after the development Interestingly, chemoresistant model acquired a distinct KRASQ61R mutation that...
Abstract PARP inhibitors have gained recent attention due to their highly selective killing of BRCA1/2-mutated and DNA double-strand break (DSB) repair–deficient tumors. Unfortunately, the majority sporadic breast cancers carry wild-type BRCA1/2 are proficient in DSB repair. We others shown that BRCA1 is a nuclear/cytoplasm shuttling protein transiently exported from nucleus cytosol upon various stimuli. Thus, we hypothesized depletion nuclear would compromise repair subsequently render...
Nurses are key providers in the care of children with mild traumatic brain injury (mTBI). New treatment recommendations emphasize symptom assessment and rest guidelines to optimize recovery. This study compared pediatric trauma core nurses' knowledge, degree confidence, perceived change practice following mTBI education.Twenty-eight nurses were invited participate this voluntary quasiexperimental, one-group pretest-posttest study. Multiple choice questions developed assess self-report Likert...
Lassa virus (LASV) is the causative agent of fever (LF), which presents as a lethal hemorrhagic disease in severe cases. LASV-induced hearing loss survivors huge socioeconomic burden, however, mechanism(s) leading to unknown. In this study, we evaluate mouse LF model auditory function using brainstem response (ABR) and distortion product otoacoustic emissions (DPOAE) determine mechanisms underlying loss. process, pioneered measures ABR DPOAE tests rodents biosafety level 4 (BSL-4)...
Abstract Aim Deregulated signaling pathways are a hallmark feature of oncogenesis and driver tumor progression. Dual specificity protein phosphatase 4 (DUSP4) is critical negative regulator the mitogen-activated kinase (MAPK) pathway often deleted or epigenetically silenced in tumors. DUSP4 alterations lead to hyperactivation MAPK many cancers, including breast cancer, which harbor mutations cell cycle checkpoint genes, particularly TP53. Methods Using genetically engineered mouse model, we...
Loss of retinoblastoma protein (RB1) function is a major driver in cancer development. We have recently reported that, addition to its well-documented functions cell cycle and fate control, RB1 paralogs novel role regulating DNA repair by non-homologous end joining (NHEJ). Here we summarize our findings present mechanistic hypotheses on how may support the process therapeutic implications for patients who harbor RB1-negative cancers.
With altered sense of taste being a common symptom coronavirus disease 2019 (COVID-19), our objective was to investigate the presence and distribution severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) within tongue over course infection.