A5083470437- Soil and Unsaturated Flow
- Microbial Community Ecology and Physiology
- Clay minerals and soil interactions
- Radioactive contamination and transfer
- Groundwater flow and contamination studies
- Biocrusts and Microbial Ecology
- Mineral Processing and Grinding
- Radioactivity and Radon Measurements
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Concrete and Cement Materials Research
- Mine drainage and remediation techniques
- Legionella and Acanthamoeba research
- Nuclear and radioactivity studies
- Graphite, nuclear technology, radiation studies
- Geology and Paleoclimatology Research
- Geochemistry and Geologic Mapping
- Iron and Steelmaking Processes
- Building materials and conservation
Technical University of Liberec
2021-2025
This study evaluates the effectiveness of two standard sterilization methods on microorganisms in bentonite, which is proposed as a buffer around metal canisters containing long-lived radioactive waste. Bentonite, natural clay, contains with enhanced resistance to harsh conditions and ability reactivate upon decompaction. Sterile controls are crucial experiments estimating impact nuclear waste repositories. Yet, common bentonite has not been fully evaluated. dry heat (nine cycles at 121°C...
Bentonite buffers at temperatures beyond 100 °C could reduce the amount of high-level radioactive waste in a deep geological repository. However, it is necessary to demonstrate that buffer surrounding canisters withstands such elevated temperatures, while maintaining its safety functions (regarding long-term performance). For this reason, an experiment with thermal loading bentonite powder 150 was arranged. The paper presents changes Czech Mg/Ca underwent during heating for one year. These...
Abstract As bentonite hosts a diverse spectrum of indigenous microorganisms with the potential to influence long-term stability deep geological repositories, it is essential understand factors influencing microbial activity under repository conditions. Here, we focus on two factors, i.e., temperature and swelling pressure, using suspension Cerny Vrch boost evaluate response. Suspensions were exposed either different pressures (10, 12 15 MPa; simulate effect pressure) or elevated temperatures...
Bentonite is a crucial part of the engineered barrier system (EBS) deep geological repositories (DGR) for nuclear waste. However, as natural material, it harbors diverse indigenous microorganisms whose metabolic activity might compromise long-term integrity EBS. One critical questions microbial prediction in DGR reaction to early hot phase waste repository evolution. Our study investigates impact gamma radiation and heat on survivability bentonite (Czech BCV reference MX-80), considering...
Abstract Bentonite is an integral part of the engineered barrier system (EBS) in deep geological repositories (DGRs) for nuclear waste, but its indigenous microorganisms may jeopardize long-term EBS integrity. To predict microbial activity DGRs, it essential to understand reactions early hot phase DGR evolution. Two bentonites (BCV and MX-80) with varied bentonite/water ratios saturation levels (compacted 1600 kg.m-3 dry density/powder/suspension), were subjected heat (90°C or 150°C)...
Abstract Bentonite is an integral part of the engineered barrier system (EBS) in deep geological repositories (DGR) for nuclear waste, but its indigenous microorganisms may jeopardize long-term EBS integrity. To predict microbial activity DGRs, it essential to understand reactions early hot phase DGR evolution. Two bentonites (BCV and MX-80) with varied bentonite/water ratios saturation levels (compacted 1600 kg.m − 3 dry density/powder/suspension), were subjected heat (90–150 °C)...
Abstract Approaches to DNA extraction play a crucial role in determining the variability of results obtained through 16S rRNA amplicon sequencing. Particularly, clay‐rich samples can impede efficiency various standard cultivation‐independent techniques. We conducted an inter‐laboratory comparison study thoroughly assess efficacy two published methods (kit‐based and phenol‐chloroform‐based) specifically designed for bentonite samples. To this end, we spiked Wyoming MX 80 with different mock...
Abstract As bentonite hosts a diverse spectrum of indigenous microorganisms with the potential to influence long-term stability deep geological repositories, it is essential understand factors determining microbial activity under repository conditions. Here, we focus on two such factors, temperature and swelling pressure, using suspension Cerny Vrch boost visualise response. Suspensions were exposed differing pressures (10–15 MPa; simulating effect pressure) temperatures (60–90°C), followed...
<p>Bentonite is a swelling clay, consisting mainly of montmorillonire,  being planned to be used as backfill material in the nuclear waste repository. It contains indigenous microbial populations that can negatively influence long-term safety geological repository due their metabolic activity (canister corrosion, illitization bentonite, gas production, degradation cementitious materials). However, reliable detection microorganisms clayish generally very difficult....