A5103124159- Plant Reproductive Biology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Gene expression and cancer classification
- Plant and animal studies
- Genetics, Bioinformatics, and Biomedical Research
- Machine Learning in Bioinformatics
- Insect-Plant Interactions and Control
- Plant Physiology and Cultivation Studies
- Soybean genetics and cultivation
- Graphene and Nanomaterials Applications
- Nanotechnology research and applications
- Weed Control and Herbicide Applications
- Plant Parasitism and Resistance
- RNA Research and Splicing
- Plant tissue culture and regeneration
- Nanoparticles: synthesis and applications
- Genomics and Phylogenetic Studies
- Magnetic and Electromagnetic Effects
- Plant biochemistry and biosynthesis
- Polysaccharides and Plant Cell Walls
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
State Key Laboratory of Genetic Engineering
2010-2023
Fudan University
2012-2023
Tianjin Research Institute of Water Transport Engineering
2022
China Agricultural University
2007-2012
University of California, Riverside
2007-2012
Plant (United States)
2007-2012
Institut des Sciences des Plantes de Montpellier
2007
There is growing attention toward closed biological genomes in the environment and health. To explore reveal intergroup differences among different samples or environments, it crucial to discover biomarkers with statistical groups. The application of Linear discriminant analysis Effect Size (LEfSe) can help find good biomarkers. Based on original genome data, quality control, quantification sequences based taxa genes are carried out. First, Kruskal-Wallis rank test was used distinguish...
High temperatures have a great impact on plant reproductive development and subsequent fruit seed set, but the underlying molecular mechanisms are not well understood. We used transcriptome profiling to investigate effect of heat stress Arabidopsis thaliana plants observed distinct response patterns in vegetative versus tissues. Exposure affected developmental programs, including early phases anther/ovule meiosis. Also, genes participating unfolded protein (UPR) were enriched tissue-specific...
The anther is the male reproductive organ of flowering plants, and Arabidopsis bHLH transcription factors encoded by DYSFUNCTIONAL TAPETUM1 (DYT1) ABORTED MICROSPORE (AMS) are required for control complex transcriptional networks regulating development. Knowledge mechanisms which proteins affect this diverse gene expression quite limited. We examine here three recently duplicated genes, bHLH010, bHLH089 bHLH091, using evolutionary, genetic, morphological transcriptomic approaches, uncover...
Transcriptional regulation is one of the most important mechanisms controlling development and cellular functions in plants animals. The Arabidopsis thaliana bHLH transcription factor (TF) DYSFUNCTIONL TAPETUM1 (DYT1) required for normal male fertility anther activates expression bHLH010/bHLH089/bHLH091 genes. Here, we showed that DYT1 localized to both cytoplasm nucleus at stage 5 but specifically 6 onward. proteins have strong nuclear localization signals, interact with DYT1, facilitate...
There is growing attention toward closed biological genomes in the environment and health. To explore reveal intergroup differences among different samples or environments, it crucial to discover biomarkers with statistical groups. The application of Linear discriminant analysis Effect Size (LEfSe) can help find good biomarkers. Based on original genome data, quality control, quantification sequences based taxa genes are carried out. First, Kruskal-Wallis rank test was used distinguish...
Flower development is a complex process requiring proper spatiotemporal expression of numerous genes. Accumulating evidence indicates that epigenetic mechanisms, including DNA methylation, play essential roles in modulating gene expression. However, few studies have examined the relationship between methylation and floral on genomic scale. Here we present detailed analyses methylomes at single-base resolution for three Arabidopsis periods: meristems, early flowers late flowers. We detected...
Grain weight (GW) is one of the component traits wheat yield. Existing reports have shown that multiple phytohormones are involved in regulation GW different crops. However, potential role jasmonic acid (JA) remains unclear. Here, we report triticale grain 1 (tgw1) mutant, with marked reductions both and JA content, caused by a premature stop mutation keto-acyl thiolase 2B (KAT-2B) β-oxidation during synthesis. KAT-2B overexpression increases wild type boosts Additionally, compliments defect...
Alternative splicing (AS) is a process in eukaryotic gene expression, which the primary transcript of multi-exon spliced into two or more different mature transcripts, thereby increasing proteome diversity. AS often regulated differentially between tissues developmental stages. Recent studies suggested that up to 60% intron-containing genes Arabidopsis thaliana undergo AS. Yet little known about this complicated and important during floral development. To investigate preferential expression...
Significance MicroRNAs (miRNAs) are indispensable regulators of gene expression. Hyponastic Leaves 1 (HYL1) is a core component the microprocessor that processes primary miRNAs (pri-miRNAs) into mature miRNAs. Here we report noncanonical function HYL1 in protecting pri-miRNAs from unwanted nuclear exosome activities during processing. Our observations also suggest may contribute differently to Dicer-like (DCL1)-mediated maturation loop-to-base– and base-to-loop–processed pri-miRNAs. This...
Summary Male sterility is an important tool for plant breeding and hybrid seed production. Male‐sterile mutants are largely due to abnormal development of either the sporophytic or gametophytic anther tissues. Tapetum, a key tissue, provides nutrients pollen development, its delayed degeneration induces abortion. Numerous bHLH proteins have been documented participate in tapetum angiosperms, but relatively little attention has given evolution involved developmental pathways across phylogeny...
Abstract A tip‐focused Ca 2+ gradient is tightly coupled to polarized pollen tube growth, and tip‐localized influxes of extracellular are required for this process. However the molecular identity regulation potential channels remains elusive. The present study has implicated CNGC18 (cyclic nucleotide‐gated channel 18) in because its overexpression induced wider shorter tubes. Moreover, depolarization growth was suppressed by lower calcium ([Ca ] ex ). CNGC18‐yellow fluorescence protein (YFP)...
The flower is essential for sexual reproduction of flowering plants and has been extensively studied. However, it still not clear how many genes are expressed in the flower. Here, we performed RNA-seq analysis as a highly sensitive approach to investigate Arabidopsis floral transcriptome at three developmental stages. We provide evidence that least 23, 961 active flower, including 8512 have reported florally previously. compared gene expression different stages found encoding transcription...
Accurately identifying DNA polymorphisms can bridge the gap between phenotypes and genotypes is essential for molecular marker assisted genetic studies. Genome complexities, including large-scale structural variations, bring great challenges to bioinformatic analysis obtaining high-confidence genomic variants, as sequence differences non-allelic loci of two or more genomes be misinterpreted polymorphisms. It important correctly filter out artificial variants avoid false genotyping estimation...
Early anther morphogenesis is a crucial process for male fertility in plants, governed by the transcription factor SPL. While involvement of AGAMOUS (AG) SPL activation and microsporogenesis initiation well established, our understanding mechanisms governing spatial distribution precise expression during cell fate determination remains limited. Here, we present novel findings on abnormal phenotypes two previously unreported mutants, spl-4 spl-5, morphogenesis. Through comprehensive analysis,...
The CLAVATA3/ESR-RELATED (CLE) peptide signals are required for cell-cell communication in several plant growth and developmental processes. However, little is known regarding the possible functions of CLEs anther. Here, we show that a T-DNA insertional mutant, dominant-negative (DN) overexpression (OX) transgenic plants CLE19 gene, exhibited significantly reduced anther size pollen grain number abnormal wall formation Arabidopsis (Arabidopsis thaliana). Interestingly, DN-CLE19 grains showed...
The pollen wall is a specialized extracellular cell that protects male gametophytes from various environmental stresses and facilitates pollination. Here, we reported bHLH010 bHLH089 together are required for the development of by regulating their specific downstream transcriptional metabolic networks. Both exine intine structures bhlh010 bhlh089 grains were severely defective. Further untargeted metabolomic transcriptomic analyses revealed accumulation morphogenesis-related metabolites,...
Organogenesis in plants occurs across all stages of the life cycle. Although previous studies have identified many genes as important for either vegetative or reproductive development at RNA level, global information on translational and post-translational levels remains limited. In this study, six Arabidopsis stages/organs were analyzed using quantitative proteomics phosphoproteomics, identifying 2187 non-redundant proteins evidence 1194 phosphoproteins. Compared to expression observed...
Metallic nanoparticles can be synthesised in living plants, which provide a friendly approach. In this work, the authors aimed to study synthesis of silver (AgNPs) Arabidopsis and two-dimensional (2D) distribution Ag other elements (Ca, P, S, Mg, CI) plant tissues. The concentrations tissues were determined by inductively coupled plasma-atomic emission spectrometer, showing that majority was retained roots. Transmission electron micrographs showed morphology AgNPs location cells....