A5090737639- Mass Spectrometry Techniques and Applications
- Single-cell and spatial transcriptomics
- Advanced Proteomics Techniques and Applications
- Cell Image Analysis Techniques
- Ion-surface interactions and analysis
- Advanced Biosensing Techniques and Applications
- Chemical Synthesis and Analysis
- Nicotinic Acetylcholine Receptors Study
- Metabolomics and Mass Spectrometry Studies
- Nuclear Physics and Applications
Max Planck Institute of Biochemistry
2022-2023
University of the Philippines Diliman
2020
Single-cell proteomics aims to characterize biological function and heterogeneity at the level of proteins in an unbiased manner. It is currently limited proteomic depth, throughput, robustness, which we address here by a streamlined multiplexed workflow using data-independent acquisition (mDIA). We demonstrate automated complete dimethyl labeling bulk or single-cell samples, without losing depth. Lys-N digestion enables five-plex quantification MS1 MS2 level. Because channels are...
Abstract Single-cell proteomics aims to characterize biological function and heterogeneity at the level of proteins in an unbiased manner. It is currently limited proteomic depth, throughput robustness, a challenge that we address here by streamlined multiplexed workflow using data-independent acquisition (mDIA). We demonstrate automated complete dimethyl labeling bulk or single-cell samples, without losing depth. In single runs mammalian cells, three-plex analysis tryptic peptides...
ABSTRACT Data-independent acquisition (DIA) methods have become increasingly popular in mass spectrometry (MS)-based proteomics because they enable continuous of fragment spectra for all precursors simultaneously. However, these advantages come with the challenge correctly reconstructing precursor-fragment relationships highly convoluted reliable identification and quantification. Here we introduce a scan mode combination trapped ion mobility (TIMS) parallel accumulation – serial...
Marine cone snails belonging to the Conidae family make use of neuroactive peptides in their venom capture prey. Here we report proteome profile duct Conus eburneus, a snail Tesseliconus clade. Through tandem mass spectrometry and database searching against C. eburneus transcriptome ConoServer database, identified 24 unique conopeptide sequences duct. The majority these belong T M gene superfamilies are disulfide-bonded, with cysteine frameworks V, XIV, VI/VII, III being most abundant. All...