A5055580406- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Rice Cultivation and Yield Improvement
- Insect symbiosis and bacterial influences
- Transgenic Plants and Applications
- Chromosomal and Genetic Variations
- Genetics, Aging, and Longevity in Model Organisms
- Insect Resistance and Genetics
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Plant tissue culture and regeneration
- Plant responses to water stress
- GABA and Rice Research
- Silicon Effects in Agriculture
- Viral Infectious Diseases and Gene Expression in Insects
- Legume Nitrogen Fixing Symbiosis
- Agricultural pest management studies
- Photosynthetic Processes and Mechanisms
- Retinal Development and Disorders
- Nanoparticles: synthesis and applications
- Agricultural Systems and Practices
- Hydrology and Watershed Management Studies
- Biofuel production and bioconversion
- Selenium in Biological Systems
- Water Quality Monitoring Technologies
Oak Ridge National Laboratory
2020-2024
The University of Melbourne
2018-2024
Patuakhali Science and Technology University
1970-2024
Pusan National University
2023
Bethel University
2023
Anglia Ruskin University
2022
Bangladesh University of Engineering and Technology
2021
Bangladesh University of Business and Technology
2020
University of Dhaka
2017-2018
Bangladesh Council of Scientific and Industrial Research
2012
Plants are complex systems hierarchically organized and composed of various cell types. To understand the molecular underpinnings plant systems, single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for revealing high resolution gene expression patterns at cellular level investigating cell-type heterogeneity. Furthermore, scRNA-seq analysis biosystems great potential generating new knowledge to inform design synthetic biology, which aims modify plants...
Abstract Green fluorescent protein (GFP) has been widely used for monitoring gene expression and localization in diverse organisms. However, highly sensitive imaging equipment, like fluorescence microscope, is usually required the visualization of GFP, limitings its application to fixed locations samples. A reporter that can be visualized real-time regardless shape, size location target samples will increase flexibility efficiency research work. Here, we report a GFP-like protein, called...
Virus-assisted delivery of the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system represents a promising approach for editing plant genomes. Among CRISPR/Cas systems, CRISPR/Cas9 is most widely used; however, to pack relatively large size into viral vectors with confined packaging capacity challenging. To address this technical challenge, we developed strategy based on split inteins that splits required components across dual-vector system. The...
Multiplexed CRISPR technologies have great potential for pathway engineering and genome editing. However, their applications are constrained by complex, laborious time-consuming cloning steps. In this research, we developed a novel method, PARA, which allows the one-step assembly of multiple guide RNAs (gRNAs) into vector with up to 18 gRNAs. Here, demonstrate that PARA is capable efficient transfer RNA/Csy4/ribozyme-based gRNA arrays. To aid in process streamline construction, user-friendly...
Plants adapt to their changing environments by sensing and responding physical, biological, chemical stimuli. Due sessile lifestyles, plants experience a vast array of external stimuli selectively perceive respond specific signals. By repurposing the logic circuitry biological molecular components used in nature, genetically encoded plant-based biosensors (GEPBs) have been developed directing signal recognition mechanisms into carefully assembled outcomes that are easily detected. GEPBs...
Abstract The ability to stack multiple genes in plants is of great importance the development crops with desirable traits but can be challenging due limited selectable marker options. Here we establish split systems using protein splicing elements called “inteins” for Agrobacterium -mediated co-transformation plants. First, show that such a system used effectively reconstitute visible marker, RUBY, from two non-functional fragments through tobacco leaf infiltration. Next, determine general...
CRISPR/Cas has recently emerged as the most reliable system for genome engineering in various species. However, concerns about risks associated with technology are increasing on potential unintended DNA changes that might accidentally arise from CRISPR gene editing. Developing a can detect and report presence of active tools biological systems is therefore very necessary. Here, we developed four real-time detection spontaneously indicate CRISPR-Cas editing regulation including CRISPR/Cas9...
Suppression of immune response is a phenomenon that enables biological processes such as gamete fertilization, cell growth, proliferation, endophyte recruitment, parasitism, and pathogenesis. Here, we show for the first time Plasminogen-Apple-Nematode (PAN) domain present in G-type lectin receptor-like kinases essential immunosuppression plants. Defense pathways involving jasmonic acid ethylene are critical plant immunity against microbes, necrotrophic pathogens, parasites, insects. Using...
A study was conducted to screen out the low-temperature tolerant Boro rice seedlings from November 2012 January 2013 for facing upcoming production challenge in Northwest Bangladesh. The experimental time characterized by a prevailing low environmental temperature of below 15°C. Five cultivars (V1: BR-2; V2: BR-16; V3: Pariza; V4: Minicate; V5: BRRI dhan 50) were selected study. leaf proline, chlorophyll content and total carotenoid investigated. V2 (BR-16) seedling synthesized higher...
Information on the patterns of genetic variation and population structure is essential for rational use efficient management germplasms. It helps in monitoring germplasm can also be used to predict potential gains. Therefore, present study diversity 59 rice genotypes were assessed using 8 simple sequence repeat (SSR) primers. By DNA profiling, a total 114 alleles detected. Allele number per/locus ranged from 9 27, with an average 14.25. Average polymorphism information content (PIC) value...
The study of several soil series Tista Floodplain soils were carried out to find the changing trend nutrients status from 1996 2016.The studied values different quality variables changed likewise pH 5.87 ± 0.47 5.95 0.33, organic matter 1.37 0.31% 1.47 0.41%, ,total nitrogen 0.49 0.13 cmol/kg 0.64 0.07 , phosphorous 0.10 0.04 3.19 Original Research Article 0.34 cmol/kg, potassium 0.31 0.29 2.93 0.26 sulfur 0.19 0.05 8.66 2.74 iron 1.09 0.46 2.07 0.99 manganese 0.02 0.30 copper 0.03± 0.01...
As part of a publicly funded initiative to develop genetically engineered Brassicas (cabbage, cauliflower, and canola) expressing Bacillus thuringiensis Cry-encoded insecticidal (Bt) toxin for Indian Australian farmers, we designed several constructs that drive high-level expression modified Cry1B Cry1C genes (referred as Cry1BM Cry1CM). The two main motivations modifying the DNA sequences these were minimise any licencing cost associated with commercial cultivation transgenic crop plants...
Abstract Waterlogging (WL) refers to the process by which water flow is resisted in vertical and horizontal directions thus stagnates for a short or long span of time; it induced combination human natural factors. In southwestern region Bangladesh, including Natore District, WL significant issue that needs be addressed if agricultural activity successful. This study aimed identify surface District characterise scenario area terms hydrogeology. Waterlogged areas were identified with...