A5031765688- Signaling Pathways in Disease
- Ubiquitin and proteasome pathways
- Cancer Mechanisms and Therapy
- Antifungal resistance and susceptibility
- Cell death mechanisms and regulation
- interferon and immune responses
- Toxin Mechanisms and Immunotoxins
- Fungal Infections and Studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Inflammasome and immune disorders
- Advanced Glycation End Products research
- Neonatal Respiratory Health Research
- Peptidase Inhibition and Analysis
- Research on Leishmaniasis Studies
- Asthma and respiratory diseases
- T-cell and B-cell Immunology
- Plant Virus Research Studies
- Plant Pathogenic Bacteria Studies
- Antimicrobial Peptides and Activities
- Oceanographic and Atmospheric Processes
- Synthesis and biological activity
- Streptococcal Infections and Treatments
- Autophagy in Disease and Therapy
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer Immunotherapy and Biomarkers
University of Perugia
2000-2014
Istituto Superiore di Sanità
2008-2014
Sapienza University of Rome
2009
Significance Chronic granulomatous disease (CGD) has an immunodeficiency component and, in addition, autoinflammatory which autophagy and inflammasome activation are linked amenable to IL-1 blockade. This study provides a rationale perform clinical trials investigate the efficacy of blocking CGD colitis expands therapeutic potential antagonists inflammatory diseases with defective autophagy.
Abstract The possible existence of yet undiscovered human tumorigenic viruses is still under scrutiny. development large-scale sequencing technologies, coupled with bioinformatics techniques for the characterization metagenomic sequences, have provided an invaluable tool detection unknown, infectious, agents, as demonstrated by several recent studies. However, discoveries novel possibly associated tumorigenesis are scarce at best. Here, we apply a rigorous workflow to investigate in depth...
Since IL-37 transgenic mice possesses broad anti-inflammatory properties, we assessed whether recombinant affects inflammation in a murine model of invasive pulmonary aspergillosis. Recombinant human was injected intraperitoneally into prior to infection and the effects on lung inflammasome activation were evaluated. markedly reduced NLRP3-dependent neutrophil recruitment steady state mRNA levels IL-1β production mitigated damage relevant clinical model, namely aspergillosis with cystic...
Mutations in the cystic fibrosis (CF) transmembrane conductance regulator affect innate epithelial immune function of lung, resulting exaggerated and ineffective airway inflammation that fails to eradicate pathogenic fungi. The appreciation whether such fungi are primarily responsible for or a consequence is important future therapeutics development.To characterize impact tryptophan/kynurenine pathway on preventing effective fungal clearance CF.We studied expression indoleamine...
A murine model of infection, in which immunocompetent or immunosuppressed interleukin-6-deficient (IL-6(-/-)) mice were infected intranasally with Aspergillus fumigatus conidia and monitored for parameters fungal colonization innate adaptive immunity, was used to assess the role IL-6 invasive pulmonary aspergillosis (IPA). The results indicate that IL-6(-/-) more susceptible than wild-type IPA. Susceptibility associated increased inflammatory pathology, decreased antifungal effector...
Aspergillus fumigatus is a model fungal pathogen and common cause of infection in individuals with the primary immunodeficiency chronic granulomatous disease (CGD). Although primarily considered deficiency innate immunity, CGD also linked to dysfunctional T cell reactivity. Both CD4(+) CD8(+) cells mediate vaccine-induced protection from experimental aspergillosis, but molecular mechanisms leading generation protective immunity whether these are dysregulated have not been determined. Here,...
ABSTRACT Caspase 1, formerly designated interleukin 1β (IL-1β)-converting enzyme, processes pro-IL-1β and pro-IL-18 to yield active cytokines that play a pivotal role in inflammation cell activation. We show here the effect of caspase 1 deficiency on inflammatory adaptive immune responses fungus Candida albicans . did not affect susceptibility primary systemic infection with fungus, as revealed by survival fungal growth. However, Th1-mediated resistance reinfection was greatly impaired...
Rationale: Hypoxia regulates the inflammatory-antiinflammatory balance by receptor for advanced glycation end products (RAGE), a versatile sensor of damage-associated molecular patterns. The multiligand nature RAGE places this in midst chronic inflammatory diseases.Objectives: To characterize impact hypoxia-RAGE pathway on pathogenic airway inflammation preventing effective pathogen clearance cystic fibrosis (CF) and elucidate potential role danger signal pathogenesis therapy lung...
Abstract The unresponsiveness of metastatic melanoma to conventional chemotherapeutic and biological agents is largely due the development resistance apoptosis. Pyrimethamine belongs group antifolate drugs, in addition antiprotozoan effects, it exerts a strong proapoptotic activity, which we recently characterized human T lymphocytes. However, no data regarding pyrimethamine anticancer activity are available thus far. To this end, examined vitro effects on apoptosis, cell cycle distribution,...
This study investigated the possible mechanisms underlying paradoxical caspofungin activity in vivo preclinical aspergillosis. We evaluated of escalating doses different models invasive aspergillosis, including mice deficient for selected innate immune receptors. The therapeutic efficacy experimental aspergillosis was strictly dose dependent, being observed at 0.1 and 1 mg/kg body weight depending on models. Paradoxical increase pulmonary fungal burden as well inflammatory pathology highest...
Fungal vaccines have long been a goal in the fields of immunology and microbiology to counter high mortality morbidity rates owing fungal diseases, particularly immunocompromised patients. However, design effective vaccination formulations for durable protection different fungi has lagged behind due important differences among their biology our limited understanding complex host-pathogen interactions immune responses. Overcoming these challenges is expected contribute improved strategies...
<div>Abstract<p>The unresponsiveness of metastatic melanoma to conventional chemotherapeutic and biological agents is largely due the development resistance apoptosis. Pyrimethamine belongs group antifolate drugs, in addition antiprotozoan effects, it exerts a strong proapoptotic activity, which we recently characterized human T lymphocytes. However, no data regarding pyrimethamine anticancer activity are available thus far. To this end, examined <i>in vitro</i>...
Supplementary Figure 2 from Pyrimethamine Induces Apoptosis of Melanoma Cells via a Caspase and Cathepsin Double-Edged Mechanism
Supplementary Figure 1 from Pyrimethamine Induces Apoptosis of Melanoma Cells via a Caspase and Cathepsin Double-Edged Mechanism
Supplementary Figure 2 from Pyrimethamine Induces Apoptosis of Melanoma Cells via a Caspase and Cathepsin Double-Edged Mechanism
Supplementary Figure 1 from Pyrimethamine Induces Apoptosis of Melanoma Cells via a Caspase and Cathepsin Double-Edged Mechanism
Supplementary Figure 3 from Pyrimethamine Induces Apoptosis of Melanoma Cells via a Caspase and Cathepsin Double-Edged Mechanism
Supplementary Methods and Materials from Pyrimethamine Induces Apoptosis of Melanoma Cells via a Caspase Cathepsin Double-Edged Mechanism
Supplementary Figure Legends 1-3 from Pyrimethamine Induces Apoptosis of Melanoma Cells via a Caspase and Cathepsin Double-Edged Mechanism
<div>Abstract<p>The unresponsiveness of metastatic melanoma to conventional chemotherapeutic and biological agents is largely due the development resistance apoptosis. Pyrimethamine belongs group antifolate drugs, in addition antiprotozoan effects, it exerts a strong proapoptotic activity, which we recently characterized human T lymphocytes. However, no data regarding pyrimethamine anticancer activity are available thus far. To this end, examined <i>in vitro</i>...