A5102721899- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Plant Reproductive Biology
- Metabolism and Genetic Disorders
- Plant and animal studies
- Plant Molecular Biology Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Soybean genetics and cultivation
- Pancreatic function and diabetes
- DNA Repair Mechanisms
- Cardiac electrophysiology and arrhythmias
- Plant Diversity and Evolution
- ATP Synthase and ATPases Research
- Cardiovascular Function and Risk Factors
- Plant biochemistry and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- RNA regulation and disease
- Metabolism, Diabetes, and Cancer
- Insect and Pesticide Research
- Plant Genetic and Mutation Studies
- interferon and immune responses
- Adipose Tissue and Metabolism
- Genomics, phytochemicals, and oxidative stress
- Lipid metabolism and biosynthesis
- DNA and Nucleic Acid Chemistry
Lomonosov Moscow State University
2020-2024
Federal Almazov North-West Medical Research Centre
2021-2024
Institute of Cytology and Genetics
2023
Siberian Institute of Plant Physiology and Biochemistry
2004-2021
Saint Petersburg State Pediatric Medical University
2017
Siberian Branch of the Russian Academy of Sciences
2011
The concept of ROS as an important factor controlling pollen germination and tube growth has become generally accepted in the last decade. However, relationship between various their significance for success vivo fertilization remained unexplored. For present study, we collected Nicotiana tabacum stigma exudate on different stages maturity before after pollination. Electron paramagnetic resonance (EPR) colorimetric analysis were used to assess levels O•2- H2O2 stigma. Superoxide dismutase...
Empagliflozin, an inhibitor of sodium-glucose co-transporter 2 (iSGLT2), improves cardiovascular outcomes in patients with and without diabetes possesses antiarrhythmic activity. However, the mechanisms these protective effects have not been fully elucidated. This study aimed to explore impact empagliflozin on ion channel activity electrophysiological characteristics ventricular myocardium. The main cardiac ionic currents (INa, ICaL, ICaT, IKr, IKs) action potentials (APs) were studied...
Abstract Reactive oxygen species (ROS) are a key regulator of physiological processes in pollen grains, and an essential component stigma exudate. The mechanisms this redox‐based regulatory system its features different plant groups still unclear. For two from families (tobacco lily), the dynamics total ROS, O • 2 − generation, H concentration exudate were examined using EPR spectroscopy quantitative colorimetric analysis. Dynamics all major enzymes redox homeostasis analysed native...
Introduction. The development of heart failure is closely associated with the appearance life threatening arrhythmias, which are often a terminal event for these patients. An analysis randomized clinical trials inhibitors sodium-glucose cotransporter type 2 indicates clinically significant potential drugs as agents antiarrhythmic properties. However, at moment full mechanism by this effect can be realized still not fully understood. Aim. To evaluate empagliflozin on transmembrane calcium...
ROS are known to be accumulated in stigmas of different species and can possibly perform functions important for plant reproduction. Here we tested the assumption that one their is control membrane potential provoke synthesis unique proteins germinating pollen. We used spectrofluorometry spectrophotometry detect H2 O2 stigma exudate, quantitative fluorescent microscopy pollen tubes flow cytometry protoplasts reveal effects on potential, a label-free quantification approach study proteome...
Pollen germination in vivo on wet stigmas is assisted by the receptive fluid-stigma exudate. Its exact composition still unknown because only some components have been studied. For first time, hormonal screening was carried out, and fatty acid (FA) of lipid-rich (Nicotiana tabacum) sugar-rich (Lilium longiflorum) exudates Screening exudate for presence plant hormones using HPLC-MS revealed abscisic (ABA) tobacco stigma at two stages development, pre-maturity mature awaiting pollination,...
Pollen is a highly specialized structure for sexual plant reproduction. Early stages of pollen germination require the transition from dormant state to active metabolism. In particular, an important role during this early phase angiosperm played by H+-ATPase. Very little known about activation in gymnosperm species, and information on involvement H+-ATPase lacking. We tracked four indicators characterizing physiological pollen: membrane potential, intracellular pH, anion efflux oxygen...
Background: Brugada syndrome (BrS) is a rare inherited cardiac arrhythmia with increased risk of sudden death. Mutations in gene SCN5A, which encodes the α-subunit voltage-gated sodium channel NaV1.5, have been identified over 20% patients BrS. However, only small fraction NaV1.5 variants, are associated BrS, characterized electrophysiological experiments. Results: Here we explored variants V281A and L1582P, were found our F543L K1419E, reportedly Heterologous expression CHO-K1 cells Western...
Background. We used the “Mitochondrial Membrane Potential Kit” (Sigma-Aldrich) to detected MMP but encountered difficulties by applied this kit because in manual there not JC-10 concentration and it allowing for account cell type, size, density, differences incubation time different cultures. Objective. Adaptation of method determining (mitochondrial membrane potential) C2C12 cells using microplate reader electron microscope. Design methods. was measured two way, fluorescence microscopy...
The latest scientific data of the development, structure and cellular composition thymus are studied presented. Both widely known sources new literature were used. This article is reporting external features thymus, internal structure, composition. In particular, attention paid to description zones, because there has been no consensus among morphologists in this matter. Embryonic development described, as well morphometric parameters different periods prenatal development. will be useful...