A5068078701- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- Supramolecular Self-Assembly in Materials
- Biochemical and Structural Characterization
- Hydrogels: synthesis, properties, applications
- Glycosylation and Glycoproteins Research
- Cellular Mechanics and Interactions
- Advanced Materials and Mechanics
- Cell Adhesion Molecules Research
- Microfluidic and Bio-sensing Technologies
- Molecular Junctions and Nanostructures
- Enzyme Production and Characterization
- Ubiquitin and proteasome pathways
- Silk-based biomaterials and applications
Arizona State University
2018-2025
Tempe Union High School District
2018
DNA and peptides are two of the most commonly used biomolecules for building self-assembling materials, but few examples exist hybrid nanostructures that contain both components. Here we report modification comprise a coiled-coil heterodimer pair with unique handles in order to link origami bearing complementary strands into micrometer-long one-dimensional arrays. We probed effect number coils on self-assembly demonstrated formation structures through multiple routes: one-pot assembly,...
Abstract The focal adhesion kinase (FAK) scaffold provides FAK-targeted cancer therapeutics with greater efficacy and specificity than traditional inhibitors. FAK function largely involves the interaction between FAK’s targeting (FAT) domain paxillin, ultimately regulating many hallmarks of cancer. We report design paxillin LD-motif mimetics that successfully inhibit FAT-paxillin interaction. Chemical biochemical screening identifies stapled peptide 1907, a high affinity binder FAT...
Abstract The programmable synthesis of rationally engineered crystal architectures for the precise arrangement molecular species is a foundational goal in nanotechnology, and DNA has become one most prominent molecules construction these materials. In particular, branched junctions have been used as central building block assembly 3D lattices. Here, crystallography to probe effect all 36 immobile Holliday junction sequences on self-assembling crystals. Contrary established paradigm field,...
Biomaterials with dynamically tunable properties are critical for a range of applications in regenerative medicine and basic biology. In this work, we show the reversible control gelatin methacrylate (GelMA) hydrogel stiffness through use DNA crosslinkers. We replaced some inter-GelMA crosslinks double-stranded DNA, allowing their removal toehold-mediated strand displacement. The could be restored by adding fresh dsDNA complementary handles to those on hydrogel. elastic modulus (G')...
The combination of multiple orthogonal interactions enables hierarchical complexity in self-assembled nanoscale materials. Here, efficient supramolecular polymerization DNA origami nanostructures is demonstrated using a multivalent display small molecule host-guest interactions. Modification strands with cucurbit[7]uril (CB[7]) and its adamantane guest, yielding complex an affinity order 10
The structural analysis of guest molecules in rationally designed and self-assembling DNA crystals has proven an elusive goal since its conception. Oligonucleotide frameworks provide especially attractive route toward studying DNA-binding by using three-dimensional lattices with defined sequence structure. In this work, we site-specifically position a suite minor groove binding molecules, solve their structures via X-ray crystallography as proof-of-principle scaffolding larger species. Two...
Integrating proteins with DNA nanotechnology would enable materials diverse applications in biology, medicine, and engineering. Here, we describe a method for incorporating bioactive fibronectin domain nanostructures using two orthogonal coiled-coil peptides. One peptide from each pair is attached to origami cuboid multivalent fashion by attaching the peptides handles. These structures can then be assembled into one-dimensional arrays through addition of linker genetically fused...
Biomaterials with dynamically tunable properties are critical for a range of applications in regenerative medicine and basic biology. In this work, we show the reversible control gelatin methacrylate (GelMA) hydrogel stiffness through use DNA crosslinkers. We replaced some inter-GelMA crosslinks double-stranded DNA, allowing their removal via toehold-mediated strand displacement. The could be restored by adding fresh dsDNA complementary handles to hydrogel. elastic modulus (G’) hydrogels...
Abstract Peptides and DNA are two of the most commonly used self‐assembling biological molecules for construction nanomaterials. However, there only a few examples that combine these self‐assembly motifs as key structural elements in nanostructure. We report on synthesis peptide−DNA conjugate self‐assembles into stable homotrimer based coiled‐coil motif. The hybrid trimer was then novel three‐way junction to link together either small tile nanostructures, or close up triangular wireframe...
The structural analysis of guest molecules in rationally designed and self-assembling DNA crystals has proven elusive since its conception. Oligonucleotide frameworks provide an especially attractive route towards studying DNA-binding by using three-dimensional lattices with defined sequence structure. In this work, we site-specifically position a suite minor groove binding molecules, solve their structures via x-ray crystallography, as proof-of-principle scaffolding larger species. Two...
DNA and peptides are two of the most commonly used biomolecules for building self-assembling materials, but few examples exist hybrid nanostructures that contain both components. Here we report modification comprise a coiled-coil heterodimer pair with orthogonal handles in order to link origami bearing complementary strands into micrometer long one-dimensional arrays. We probed effect number coils on self-assembly demonstrated formation self-assembled structures through multiple routes, form...
DNA and peptides are two of the most commonly used biomolecules for building self-assembling materials, but few examples exist hybrid nanostructures that contain both components. Here we report modification comprise a coiled-coil heterodimer pair with orthogonal handles in order to link origami bearing complementary strands into micrometer long one-dimensional arrays. We probed effect number coils on self-assembly demonstrated formation self-assembled structures through multiple routes, form...
Biomaterials with dynamically tunable properties are critical for a range of applications in regenerative medicine and basic biology. In this work, we show the reversible control gelatin methacrylate (GelMA) hydrogel stiffness through use DNA crosslinkers. We replaced some inter-GelMA crosslinks double-stranded DNA, allowing their removal via toehold-mediated strand displacement. The could be restored by adding fresh dsDNA complementary handles to hydrogel. elastic modulus (G’) hydrogels...