A5081739955- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Law, Rights, and Freedoms
- Legal and Constitutional Studies
- Judicial and Constitutional Studies
- DNA and Biological Computing
- Bacteriophages and microbial interactions
- Supramolecular Self-Assembly in Materials
- Block Copolymer Self-Assembly
- Anodic Oxide Films and Nanostructures
- American Constitutional Law and Politics
- Electoral Systems and Political Participation
- Legal Systems and Judicial Processes
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- Law in Society and Culture
- European and International Law Studies
- Silk-based biomaterials and applications
- Molecular Junctions and Nanostructures
- Urban, Neighborhood, and Segregation Studies
- Nerve injury and regeneration
- Modular Robots and Swarm Intelligence
- International Law and Human Rights
- Taxation and Legal Issues
Arizona State University
2016-2025
Harvard University Press
2024
Tempe Union High School District
2018-2023
Northwestern University
2012-2018
University of Chicago
2006-2018
Physical Sciences (United States)
2018
University of Illinois Chicago
2017
Chicago Kent College of Law
2007-2015
University of California, Berkeley
2009-2013
Lawrence Berkeley National Laboratory
2009-2011
Chemically reversible hydrogels The dynamic reorganization of some cellular biopolymers in response to signals has inspired efforts create artificial materials with similar properties. Freeman et al. created based on peptide amphiphiles that can bear DNA strands assemble into superstructures and disassemble chemical triggers. addition conjugates induced transitions from micelles fibers bundles fibers. resulting were used as an extracellular matrix mimic for cultured cells. Switching the...
Abstract Regeneration after severe spinal cord injury cannot occur naturally in mammals. Transplanting stem cells to the site is a highly promising method, but it faces many challenges because relies heavily on microenvironment provided by both lesion and delivery material. Although mechanical properties, biocompatibility, biodegradability of materials have been extensively explored, their permeability has rarely recognized. Here, DNA hydrogel designed with extremely high repair 2 mm gap...
Sophisticated statistical mechanics approaches and human intuition have demonstrated the possibility of self-assembling complex lattices or finite-size constructs. However, attempts so far mostly only been successful in silico often fail experiment because unpredicted traps associated with kinetic slowing down (gelation, glass transition) competing ordered structures. Theoretical predictions also face difficulty encoding desired interparticle interaction potential experimentally available...
Bacteriophage MS2 was used to construct a targeted, multivalent photodynamic therapy vehicle for the treatment of Jurkat leukemia T cells. The self-assembling spherical virus capsid modified on interior surface with up 180 porphyrins capable generating cytotoxic singlet oxygen upon illumination. exterior ∼20 copies Jurkat-specific aptamer using an oxidative coupling reaction targeting unnatural amino acid. capsids were able target and selectively kill more than 76% cells after only 20 min...
We report the construction of DNA nanotubes covalently functionalized with cell adhesion peptide RGDS as a bioactive substrate for neural stem differentiation. Alteration Watson–Crick base pairing program that builds nanostructures allowed us to probe independently effect nanotube architecture and bioactivity on found both factors instruct synergistically preferential differentiation cells into neurons rather than astrocytes.
Three-dimensional (3D) cages are one of the most important targets for nanotechnology. Both proteins and DNA have been used as building blocks to create tunable nanoscale a wide range applications, but each molecular type has its own limitations. Here, we report cage constructed from both protein through use covalent protein–DNA conjugates. We modified homotrimeric (KDPG aldolase) with three identical single-stranded handles by functionalizing reactive cysteine residue introduced via...
DNA origami was used as a scaffold to arrange spherical virus capsids into one-dimensional arrays with precise nanoscale positioning. To do this, we first modified the interior surface of bacteriophage MS2 fluorescent dyes model cargo. An unnatural amino acid on external then coupled strands that were complementary those extending from tiles. Two different geometries tiles (rectangular and triangular) used. The associated both virtually 100% efficiency under mild annealing conditions,...
Abstract The native extracellular matrix is a space in which signals can be displayed dynamically and reversibly, positioned with nanoscale precision, combined synergistically to control cell function. Here we describe molecular system that programmed these three characteristics. In this approach immobilize peptide-DNA (P-DNA) molecules on surface through complementary DNA tethers directing cells adhere spread reversibly over multiple cycles. also serve as ruler the distance-dependent...
Proteins and DNA are two commonly used molecules for self-assembling nanotechnology. In this tutorial review, we discuss the hybrid field of "protein-DNA nanotechnology," whereby proteins integrated with scaffolds creation nanostructures distinct properties each molecular type. We first bioconjugation strategies, both covalent supramolecular, integrating nanostructures. Next, review seminal work in four emerging areas protein-DNA nanotechnology: (1) controlling protein orientation on...
DNA and peptides are two of the most commonly used biomolecules for building self-assembling materials, but few examples exist hybrid nanostructures that contain both components. Here we report modification comprise a coiled-coil heterodimer pair with unique handles in order to link origami bearing complementary strands into micrometer-long one-dimensional arrays. We probed effect number coils on self-assembly demonstrated formation structures through multiple routes: one-pot assembly,...
DNA nanostructures (DNs) have gained popularity in various biomedical applications due to their unique properties, including structural programmability, ease of synthesis and functionalization, low cytotoxicity. Effective utilization DNs...
Abstract The focal adhesion kinase (FAK) scaffold provides FAK-targeted cancer therapeutics with greater efficacy and specificity than traditional inhibitors. FAK function largely involves the interaction between FAK’s targeting (FAT) domain paxillin, ultimately regulating many hallmarks of cancer. We report design paxillin LD-motif mimetics that successfully inhibit FAT-paxillin interaction. Chemical biochemical screening identifies stapled peptide 1907, a high affinity binder FAT...
Self-assembling, light harvesting arrays of organic chromophores can be templated using the tobacco mosaic virus coat protein (TMVP). The efficiency energy transfer within systems containing a high ratio donors to acceptors shows strong dependence on TMVP assembly state. Rod and disk assemblies derived from single stock chromophore-labeled exhibit drastically different levels transfer, with rods significantly outperforming disks. origin superior was probed through controlled introduction...
Abstract The programmable synthesis of rationally engineered crystal architectures for the precise arrangement molecular species is a foundational goal in nanotechnology, and DNA has become one most prominent molecules construction these materials. In particular, branched junctions have been used as central building block assembly 3D lattices. Here, crystallography to probe effect all 36 immobile Holliday junction sequences on self-assembling crystals. Contrary established paradigm field,...
Direct and efficient delivery of functional payloads such as chemotherapy drugs, siRNA, or small-molecule inhibitors into the cytoplasm, bypassing endo/lysosomal trapping, is a challenging task for intracellular medicine. Here, we take advantage programmability DNA nanotechnology to develop nanodevice called CytoDirect, which incorporates disulfide units human epidermal growth factor receptor 2 (HER2) affibodies origami nanostructure, enabling rapid cytosolic uptake targeted cancer cells...
Harnessing energy from within: Energy transfer the inside to outside of MS2 capsids modified by positioning fluorescent dyes and a photocatalytic zinc porphyrin on their surfaces (see picture) enabled sensitization at multiple wavelengths thus broad-spectrum catalytic activity. Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted authors. Please note: The publisher...
The foundational goal of structural DNA nanotechnology-the field that uses oligonucleotides as a molecular building block for the programmable self-assembly nanostructured systems-was to use construct three-dimensional (3D) lattices solving macromolecular structures. nature makes it an ideal system rationally constructing self-assembled crystals and immobilizing guest molecules in repeating 3D array through their specific stereospatial interactions with scaffold. In this work, we have...