A5070494102- Developmental Biology and Gene Regulation
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Wnt/β-catenin signaling in development and cancer
- scientometrics and bibliometrics research
- Genetic and Kidney Cyst Diseases
- Hippo pathway signaling and YAP/TAZ
- Research Data Management Practices
- Scientific Computing and Data Management
- Community Development and Social Impact
- Genetic Syndromes and Imprinting
- Cytomegalovirus and herpesvirus research
- Retinal Development and Disorders
- Academic Publishing and Open Access
- Health, psychology, and well-being
- Social Work Education and Practice
- Viral-associated cancers and disorders
- Renal and related cancers
- Ethics in Clinical Research
- Polyomavirus and related diseases
- Healthcare Systems and Technology
- Congenital heart defects research
- Heavy Metal Exposure and Toxicity
- Mental Health and Patient Involvement
Agency for Toxic Substances and Disease Registry
2020
Centers for Disease Control and Prevention
2020
Wellcome Trust
2016-2019
Emory University
1989-2014
The time is right for biologists to post their research findings onto preprint servers
The Epstein-Barr virus (EBV) nuclear antigen EBNA-1 plays an integral role in the maintenance of latency EBV-infected B lymphocytes. binds to sequences within plasmid origin replication (oriP). It is essential for latent episomal form EBV DNA and may also regulate expression EBNA group gene products. We have used sequence-specific DNA-binding assays purify away from nonspecific proteins a B-lymphocyte cell extract. availability this eucaryotic protein has allowed examination interaction with...
During sporulation in Bacillus subtilis, new RNA polymerase sigma factors are produced. These direct the transcription of genes that required for this cellular differentiation. In order to determine role each factor process, it is necessary know which promoters recognized by factor. The spoIIID gene product plays an important establishment mother cell-specific expression during sporulation. We found substitution alanine at position 124 sporulation-specific E suppressed effect a...
The sigma subunit of bacterial RNA polymerase is required for specific binding the enzyme to promoters. This specificity probably directed by two regions most factors that make sequence-specific contacts at promoters, -10 and -35 regions. We found a single amino acid substitution in recognition region E from Bacillus subtilis trapped stable complex with promoter DNA which it was unable initiate transcription. Our results are consistent view utilization proceeds through several intermediate...
<ns4:p>Serious concerns about the way research is organized collectively are increasingly being raised. They include escalating costs of and lower productivity, low public trust in researchers to report truth, lack diversity, poor community engagement, ethical over practices, irreproducibility. Open science (OS) collaborations comprise a subset open practices including access publication, data sharing absence restrictive intellectual property rights with which institutions, firms,...
The sigma subunit of bacterial RNA polymerase is required for specific binding to promoters. One region in most factors makes sequence-specific contacts at the -10 its cognate To test role amino acids this region, we examined effects 49 single-amino-acid substitutions E from Bacillus subtilis. We assayed effect each acid substitution on spore formation because essential endospore B. Our results showed that several positions, including highly conserved aromatic position 102, had little or no...
In the developing Drosophila eye, morphogenetic furrow is a developmental organizing center for patterning and cell proliferation. The acts both to limit eye size coordinate number of cells facets. Here we report molecular functional characterization mini-me (mnm), potential regulator proliferation survival in eye. We first identified mnm as dominant modifier hedgehog loss-of-function that encodes conserved protein with zinc knuckle RING finger domains. show dispensable disc, but required...
<ns4:p>Serious concerns about the way research is organized collectively are increasingly being raised. They include escalating costs of and lower productivity, low public trust in researchers to report truth, lack diversity, poor community engagement, ethical over practices, irreproducibility. Open science (OS) collaborations comprise a set practices including open access publication, data sharing absence restrictive intellectual property rights with which institutions, firms, governments...
The discovery of secondary sigma factors in Bacillus subtilis that enable RNA polymerase to transcribe cloned sporulation genes vitro has led the proposal appearance new during directs different temporal classes genes. One factor, which appears 2 h after initiation sporulation, is E (formerly 29). Mutations inactivate structural gene for prevent transcription from promoter G4. To determine whether E-RNA interacts with G4 vivo, we examined effects six single-base-pair substitutions on its...
We have developed and implemented normative interventions called “Rigor Transparency Initiatives”(RTIs) to shift attitudes, norms, behaviors in research communities. RTIs are usually a collaboration between one or more journals funders involving funding call for special section/issue of promote new behaviors. The purpose is spur engagement with that valued, but not yet incentivized normative, such as publishing null results replication studies, conducting preregistration Registered Reports...