A5082744894- Bladder and Urothelial Cancer Treatments
- Polyamine Metabolism and Applications
- Urinary and Genital Oncology Studies
- Renal cell carcinoma treatment
- Epigenetics and DNA Methylation
- Metabolomics and Mass Spectrometry Studies
- Inflammatory mediators and NSAID effects
- Cancer Research and Treatments
- Sperm and Testicular Function
- Cancer, Stress, Anesthesia, and Immune Response
- Drug Transport and Resistance Mechanisms
- PI3K/AKT/mTOR signaling in cancer
- Cancer Genomics and Diagnostics
- Tea Polyphenols and Effects
- Tannin, Tannase and Anticancer Activities
- Animal testing and alternatives
- Autophagy in Disease and Therapy
- Cholesterol and Lipid Metabolism
- Cancer, Lipids, and Metabolism
- Fibroblast Growth Factor Research
- Effects and risks of endocrine disrupting chemicals
- Phytoestrogen effects and research
- Male Reproductive Health Studies
- Reproductive biology and impacts on aquatic species
- Congenital Ear and Nasal Anomalies
University of Trás-os-Montes and Alto Douro
2010-2022
Centro Hospitalar de Trás os Montes e Alto Douro
2017-2021
University of Aveiro
2016
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2016
Weatherford College
2014
Medical University of Graz
2013
Several genomic regions are frequently altered and associated with the type, stage progression of urinary bladder cancer (UBC). We present characterization 5637, T24 HT1376 UBC cell lines by karyotyping, fluorescence in situ hybridization (FISH), array comparative (aCGH) multiplex ligation-dependent probe amplification (MLPA) analysis. Some cytogenetic anomalies were found three lines, such as chromosome 20 aneuploidy loss 9p21. gene loci losses (e.g. CDKN2A) gains HRAS, BCL2L1 PTPN1)...
Abstract The purpose of this study was to determine whether everolimus, a rapamycin derivative, might significantly enhance the cytotoxicity gemcitabine, an antitumor drug, in two human bladder-cancer cell lines. Human T24 and 5637 cells were incubated with gemcitabine everolimus range concentrations either alone or combination for 72 h. Flow cytometry, comet assay, MTT method optical microscopy used assess proliferation, cycle, DNA damage, morphological alterations. Gemcitabine exerted...
Summary Hepatocellular carcinoma ( HCC ) is a common malignancy with poor clinical outcome, whose histogenesis the subject of intense debate. Specifically, expression cytokeratins CK s) 7 and 19, associated aggressive biological behaviour, proposed to reflect possible progenitor cell origin or tumour dedifferentiation towards primitive phenotype. This work addresses that problem by studying s 19 in N ‐diethylnitrosamine DEN )‐induced mouse s. ICR mice were divided into six ‐exposed matched...
The aim of this paper is to analyse sunitinib malate in vitro ability enhance cisplatin cytotoxicity T24, 5637, and HT1376 human urinary bladder-cancer cell lines. Cells were treated with (3, 6, 13, 18 μ M) (1, 2, 4, 20 M), either isolation or combined, over the course 72 hours. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, acridine orange, monodansylcadaverine staining flow cytometry performed. combination index (CI) was calculated based on Chou Talalay method. In...
Background/Aim: This study aimed to evaluate the utility of several biological parameters for prediction tumor development and animal welfare in a rat model urinary bladder cancer. Materials Methods: The control group (n=9) received tap water while test (n=12) carcinogen N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) drinking water. A score sheet with variables was used monitor animals9 welfare. Body weight, food drink consumption rectal temperature were measured weekly. Blood urine samples...
Cytokeratins (CKs) 14 and 20 are promising markers for diagnosing urothelial lesions studying their prognosis histogenesis. This work aimed to study the immunohistochemical staining patterns of CK14/20 during multistep carcinogenesis leading papillary bladder cancer in a rat model. Thirty female Fischer 344 rats were divided into three groups: group 1 (control); 2, which received N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) weeks plus week without treatment; 3, BBN 8 treatment. Bladder...
The purpose of this study was to determine the efficacy naproxen, a nonsteroidal anti-inflammatory drug, on MG-63 human osteosarcoma cell line. cells were exposed naproxen in wide range concentrations 0.03, 0.05, 0.1, 0.42, 0.83, and 1.67 mg/ml for 72 h. activity assessed by following assays: morphology; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method; terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay; comet acridine orange...
This study aimed to evaluate the in vitro efficacy of carboplatin and piroxicam, both isolation combined, against T24 5637 human urinary bladder cancer cell lines.Cell viability, drug interaction, morphology, proliferation, apoptosis autophagy were analyzed after 72 h exposure. Statistical analysis was performed values p<0.05 considered statistically significant.Drug exposure combination led a significant reduction viability comparatively single-drug These combinations resulted synergistic...
A cross-sectional pilot study was conducted in men followed fertility consultations, from the portuguese Trás-os-Montes and Alto Douro region, order to associate several lifestyle factors with spermatic parameters. Of a total of 522 men, 373 were compared based on occupational exposure harmful factors, smoking habits practice physical exercise per week, other 149 according their body mass index (normal weight vs. overweight obesity). In absence seems be associated sperm quality improvement,...
This study aimed to evaluate the impact of a vapor freezing protocol on antioxidant enzyme activity (superoxide dismutase (SOD) and glutathione reductase (GR)), sperm with active mitochondria, DNA damage, spermatic parameters. Twenty-six semen samples from men undergoing infertility investigation were cryopreserved in liquid nitrogen (LN) vapors plunged into LN, (method A) without B) commercial medium (SFM) inherent removal preparation (SPM). Most parameters assessed before after freezing,...
To evaluate the in vitro effects of sunitinib malate and meloxicam isolation, to analyse ability enhance cytotoxicity three human bladder-cancer cell lines. Cell lines were treated with meloxicam, either isolation or combined. Leishman staining, MTT method, comet assay, MDC staining M30 CytoDEATH antibody performed. The Chou Talalay method was applied. Sunitinib supressed proliferation cells a concentration-dependent manner. Treatment combination showed synergistic effect. When exploring...
Abstract The 3p deletion syndrome is an unusual condition. few cases described are mainly de novo. We a familial case detected in prenatal diagnosis. Three members of the family had 3p26.3‐p26.1 deletion; however, only son presented clinical features.