A5009724866- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Bacteriophages and microbial interactions
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Insect Resistance and Genetics
- Plant Reproductive Biology
- RNA Research and Splicing
- Neurobiology and Insect Physiology Research
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Resistance and Genetics
- RNA modifications and cancer
- Plant and Fungal Interactions Research
- Plant Genetic and Mutation Studies
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- RNA and protein synthesis mechanisms
- Animal Virus Infections Studies
- Plant pathogens and resistance mechanisms
- Plant Gene Expression Analysis
- Transgenic Plants and Applications
- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Viral Infections and Immunology Research
Myongji University
2009-2020
John Innes Centre
2010
University of Dundee
2010
Korea University
1998-2005
The nucleolus and specific nucleolar proteins are involved in the life cycles of some plant animal viruses, but functions these trafficking virus infections largely unknown. ORF3 protein virus, groundnut rosette (an umbravirus), has been shown to cycle through nucleus, passing Cajal bodies then exiting back into cytoplasm. This journey is absolutely required for formation viral ribonucleoprotein particles (RNPs) that, themselves, essential spread noninoculated leaves shoot tip. Here, we show...
To test the hypothesis that caspase-like proteases exist and are critically involved in implementation of programmed cell death (PCD) plants, a search was undertaken for plant caspases activated during N gene–mediated hypersensitive response (HR; form pathogen-induced PCD plants) tobacco plants infected with Tobacco mosaic virus (TMV). For detection, characterization, partial purification caspase, Agrobacterium tumefaciens VirD2 protein, shown here to be cleaved specifically at two sites...
Abstract The eukaryotic nucleolus is multifunctional and involved in the metabolism assembly of many different RNAs ribonucleoprotein particles as well cellular functions, such cell division transcriptional silencing plants. We previously showed that Arabidopsis thaliana exon junction complex proteins associate with nucleolus, suggesting a role for mRNA production. Here, we report plant contains mRNAs, including fully spliced, aberrantly single gene transcripts. Aberrant mRNAs are much more...
Nonsense-mediated mRNA decay (NMD), an quality control process, is thought to function in plant immunity. A subset of fully spliced (FS) transcripts Arabidopsis (Arabidopsis thaliana) resistance (R) genes are upregulated during bacterial infection. Here, we report that 81.2% and 65.1% FS natural TIR-NBS-LRR (TNL) CC-NBS-LRR transcripts, respectively, retain characteristics NMD regulation, as their transcript levels could be controlled posttranscriptionally. Both infection the perception...
The capsid protein (CP) of Cucumber mosaic virus (CMV) is required for cell-to-cell movement, mediated by the 3a movement (MP). Deletion C-terminal 33 amino acids CMV MP (in mutant designated 3aDeltaC33 MP) resulted in CP-independent but not long-distance movement. RNA-binding studies done vitro using isolated bacterially expressed showed that bound RNA more strongly, with fewer regions sensitive to RNase and formed cooperatively complexes at lower ratios : than wild-type (wt) MP. Analysis...
Small nucleolar RNAs (snoRNAs) and small Cajal body-specific (scaRNAs) are non-coding whose main function in eukaryotes is to guide the modification of nucleotides ribosomal spliceosomal nuclear RNAs, respectively. Full-length sequences Arabidopsis snoRNAs scaRNAs have been obtained from cDNA libraries capped uncapped using RNA isolated nucleoli cell cultures. We identified 31 novel snoRNA genes (9 box C/D 22 H/ACA) 15 new variants previously described snoRNAs. Three related with a distinct...
Potato leafroll virus (PLRV) encodes two capsid proteins, major protein (CP) and minor (P5), an extended version of the CP produced by occasional translational 'readthrough' gene. Immunogold electron microscopy showed that PLRV is located in cytoplasm also localized nucleus, preferentially targeting nucleolus. The nucleolar localization was confirmed when it expressed as a fusion with green fluorescent (GFP) via Agrobacterium vector. Mutational analysis identified particular sequence within...
The 27 kDa protein encoded by ORF3 of Groundnut rosette virus (GRV) is required for viral RNA protection and movement through the phloem. Localization studies have revealed that this located in nuclei, preferentially targeting nucleoli. We demonstrated amino acids (aa) 108-122 GRV contain an arginine-rich nuclear localization signal. Arginine-to-asparagine substitutions region decreased level accumulation nuclei. A leucine-rich export signal (NES) was at aa 148-156 protein....
The genome of Cucumber mosaic virus consists three single-stranded RNA molecules, RNAs 1, 2 and 3. 1 encode the 1a 2a proteins, respectively, which are necessary for replication viral have been implicated in movement plants. 3a protein (MP), encoded by 3, is essential transferring genomes from infected cells to adjacent across plasmodesmata. Far-Western analysis demonstrated that bacterially expressed polymerase directly interacted with MP. Interaction was confirmed a yeast two-hybrid assay,...
Strong RNA silencing was induced in plants transformed with an amplicon consisting of full-length cDNA potato leafroll virus (PLRV) expressing green fluorescent protein (GFP), as shown by low levels PLRV-GFP accumulation, lack symptoms and accumulation amplicon-specific short interfering RNAs (siRNAs). Inoculation these various viruses known to encode suppressor proteins a striking synergistic effect leading the enhanced PLRV-GFP, suggesting that it had escaped from silencing. However,...
The tobacco bZIP transcription factor, TBZF, functions in leaf senescence and floral development. In order to identify TBZF-interaction partner(s), we screened a cDNA library constructed from leaves of (Nicotiana tabacum cv. Xanthi) by the yeast two hybrid method. Among 16 clones initially identified, one clone was further characterized because its highly specific binding bait. full length 776 bp encoded polypeptide 12.5 kDa, which designated NtTIP1 TBZF-Interaction Protein 1). A high...
We have cloned apolipophorin-III (apoLp-III) cDNA from adult fat body of Spodoptera litura. The sequence encodes a 188 amino acid polypeptide including 22 leader peptide. circular dichroism spectrum the purified apoLp-III indicated considerable content alpha-helix. Sequence alignment showed that S. litura has relatively high degree identity with apoLps-III lepidopteran, Manduca sexta (72%), Galleria mellonella (67%), Bombyx mori (60%). These alignments four lepidopteran highly identical...
The replication and transcription of cucumber mosaic virus (CMV) are catalyzed by multi-protein complex RNA-dependent RNA polymerase (RdRp), which is composed the viral-encoded 1a 2a proteins with host factors. We have reported that N-terminal region protein, 126 amino acids, required for interaction helicase phosphorylation abrogated suggesting a mechanism resistance in plants against viral infection. Here, we found three protein kinases, 60, 55, 38 kDa, co-purified tobacco membrane...
Nonsense-mediated mRNA decay (NMD), an quality control process, has been implicated in plant immunity. A subet of transcripts the Arabidopsis resistance (R) genes are reportedly upregulated during bacterial infection due to a decrease NMD efficiency. Here, we report that 81.2% and 65.1% fully spliced natural TIR-NBS-LRR (TNL) CC-NBS-LRR (CNL) transcripts, respectively, retain characteristics regulation. The perception bacteria by pattern recognition receptors (PRRs) initiates destruction...