A5077917238- Hemoglobin structure and function
- Heme Oxygenase-1 and Carbon Monoxide
- Antimicrobial Resistance in Staphylococcus
- Porphyrin Metabolism and Disorders
- Neonatal Health and Biochemistry
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Trace Elements in Health
- Redox biology and oxidative stress
- Sulfur Compounds in Biology
- Erythrocyte Function and Pathophysiology
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antibiotic Resistance in Bacteria
- Nitric Oxide and Endothelin Effects
- Cassava research and cyanide
- Salmonella and Campylobacter epidemiology
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Microbial Fuel Cells and Bioremediation
- Freezing and Crystallization Processes
- Plant Pathogenic Bacteria Studies
- Thermal Regulation in Medicine
University of Sheffield
2012-2025
Sigma (Poland)
2018
Hospital for Sick Children
2016-2018
Abstract Hydrogen sulfide (H 2 S) impairs mitochondrial respiration by potently inhibiting the heme-copper cytochrome c oxidase. Since many prokaryotes, including Escherichia (E.) coli , generate H S and encounter high levels particularly in human gut, herein we tested whether bacteria can sustain sulfide-resistant O -dependent respiration. E. has three respiratory oxidases, cyanide-sensitive bo 3 enzyme two bd oxidases much less sensitive to cyanide. Working on isolated enzymes, found that,...
The hybrid cluster protein, Hcp, contains a 4Fe-2S-2O iron-sulfur-oxygen that is currently considered to be unique in biology. It protects various bacteria from nitrosative stress, but the mechanism unknown. We demonstrate Escherichia coli Hcp high affinity nitric oxide (NO) reductase major enzyme for reducing NO stoichiometrically N2 O under physiologically relevant conditions. Deletion of hcp results extreme sensitivity during anaerobic growth and inactivation iron-sulfur proteins,...
Methicillin-resistant Staphylococcus aureus (MRSA), in which acquisition of mecA [which encodes the cell wall peptidoglycan biosynthesis component penicillin-binding protein 2a (PBP2a)] confers resistance to β-lactam antibiotics, is major clinical concern. We show that, presence MRSA adopts an alternative mode division and shows altered architecture at septum. PBP2a can replace transpeptidase activity endogenous essential PBP2 but not that PBP1, responsible for distinctive native septal...
We set out to investigate the antibacterial activity of a new Mn-based photoactivated carbon monoxide-releasing molecule (PhotoCORM, [Mn(CO)3(tpa-κ(3)N)](+)) against an antibiotic-resistant uropathogenic strain (EC958) Escherichia coli.Activated PhotoCORM inhibits growth and decreases viability E. coli EC958, but non-illuminated (CORM) is without effect. NADH-supported respiration rates are significantly decreased by activated PhotoCORM, mimicking effect dissolved CO gas. from binds...
Carbon monoxide-releasing molecules (CORMs) are a promising class of new antimicrobials, with multiple modes action that distinct from those standard antibiotics. The relentless increase in antimicrobial resistance, exacerbated by lack antibiotics, necessitates better understanding how such novel agents act and might be used synergistically established This work aimed to understand the mechanism(s) underlying synergy between manganese-based photoactivated carbon molecule (PhotoCORM), [Mn(CO)...
The spheroid bacterium
Bacterial cell division is a complex, multistage process requiring septum development while maintaining wall integrity. A dynamic, macromolecular protein the divisome, tightly controls morphogenesis both spatially and temporally, but mechanisms that tune septal progression are largely unknown. By studying conditional mutants of genes encoding DivIB, DivIC, FtsL, an essential trimeric complex central to in bacteria, we demonstrate FtsL DivIB play independent, hierarchical roles coordinating...
We review recent examples of the burgeoning literature on three gases that have major impacts in biology and microbiology. NO, CO H2S are now co-classified as endogenous gasotransmitters with profound effects mammalian physiology and, potentially, implications therapeutic applications. All well known to be toxic yet, at tiny concentrations human cell biology, play key signalling regulatory functions. may also endogenously generated microbes. NO share property being biochemically detoxified,...
Abstract Bacterial cell division is a complex, dynamic process that requires multiple protein components to orchestrate its progression. Many proteins are highly conserved across bacterial species alluding common, basic mechanism. Central transmembrane trimeric complex involving DivIB, DivIC and FtsL in Gram-positives. Here, we show distinct, essential role for survival of Staphylococcus aureus . spatially regulates peptidoglycan synthesis, consequently wall architecture, by influencing the...
CO and NO are small toxic gaseous molecules that play pivotal roles in biology as gasotransmitters. During bacterial infection, NO, produced by the host via inducible synthase, exerts critical antibacterial effects while CO, generated heme oxygenases, enhances phagocytosis of macrophages. In Escherichia coli, other bacteria fungi, flavohemoglobin Hmp is most important detoxification mechanism converting O2 to ion nitrate (NO3(-)). The protoheme binds not only but also so this ligand expected...
The microaerophilic pathogen Campylobacter jejuni possesses inducible systems for resisting NO. Two globins—Cgb (a single-domain globin) and Ctb truncated globin)—are up-regulated in response to NO via the positively acting transcription factor NssR. Our aims were determine whether these oxygen-binding globins also function severely oxygen-limited environments, as host. At growth-limiting oxygen transfer rates, bacteria more S-nitrosoglutathione (GSNO) sensitive, irrespective of presence...
<ns4:p><ns4:italic>Mycobacterium tuberculosis,</ns4:italic> the causative agent of human tuberculosis, has two proteins belonging to truncated hemoglobin (trHb) family. Mt-trHbN presents well-defined internal hydrophobic tunnels that allow O<ns4:sub>2</ns4:sub> and <ns4:sup>•</ns4:sup>NO migrate easily from solvent active site, whereas Mt-trHbO possesses interrupted by a few bulky residues, particularly tryptophan at position G8. Differential ligand migration rates detoxify...
<ns4:p><ns4:italic>Mycobacterium tuberculosis,</ns4:italic> the causative agent of human tuberculosis, has two proteins belonging to truncated hemoglobin (trHb) family. Mt-trHbN presents well-defined internal hydrophobic tunnels that allow O<ns4:sub>2</ns4:sub> and <ns4:sup>•</ns4:sup>NO migrate easily from solvent active site, whereas Mt-trHbO possesses are partially blocked by a few bulky residues, particularly tryptophan at position G8. Differential ligand migration rates detoxify...