A5079086115- Cellular Mechanics and Interactions
- 14-3-3 protein interactions
- Plant Virus Research Studies
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- biodegradable polymer synthesis and properties
- Plant Genetic and Mutation Studies
- Collagen: Extraction and Characterization
- HER2/EGFR in Cancer Research
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
Sidney Kimmel Cancer Center
2021
Thomas Jefferson University
2021
Temple University
2018-2020
DNA polymerase θ (Polθ) is a unique polymerase-helicase fusion protein that promotes microhomology-mediated end-joining (MMEJ) of double-strand breaks (DSBs). How full-length human Polθ performs MMEJ at the molecular level remains unknown. Using biochemical approach, we find helicase essential for long ssDNA overhangs which model resected DSBs. Remarkably, requires attachment, but not disordered central domain, and occurs independently ATPase activity. single-particle microscopy biophysical...
Polθ reverse transcribes RNA by undergoing a significant conformational change and promotes RNA-templated DNA repair.
POLQ is a unique multifunctional replication and repair gene that encodes for N-terminal superfamily 2 helicase C-terminal A-family polymerase. Although the function of polymerase domain has been investigated, little understood regarding domain. Multiple studies have reported θ-helicase (Polθ-helicase) unable to unwind DNA. However, it exhibits ATPase activity stimulated by single-stranded DNA, which presents biochemical conundrum. In contrast previous reports, we demonstrate Polθ-helicase...