Significance The impact of soil viruses on prokaryotic hosts and their functional processes is largely unknown. While metagenomic sequencing microbial communities enables identification linkages between hosts, this does not necessarily identify contemporary interactions. To enable a detailed analysis active virus–host interactions individual populations, we focused the critical biogeochemical process methane (CH 4 ) oxidation followed transfer carbon from to associated in situ. Analysis 13...

Abstract Viruses are abundant, ubiquitous members of soil communities that kill microbial cells, but how they respond to perturbation ecosystems is essentially unknown. Here, we investigate lineage-specific virus-host dynamics in grassland following “wet-up”, when resident microbes both resuscitated and lysed after a prolonged dry period. Quantitative isotope tracing, time-resolved metagenomics viromic analyses indicate holds diverse low biomass reservoir virions, which only subset thrives...

Here, we investigated overlooked microbes in soil, candidate phyla radiation (CPR) bacteria and Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoarchaeota, Nanohaloarchaeota (DPANN) archaea, by size fractionating small particles from an approach typically used for the recovery of viral metagenomes. Concentration these cells (<0.2 μm) allowed us to identify organisms as part rare soil biosphere sample genomes that were absent non-size-fractionated

Abstract Soil microbial communities perform critical ecosystem services through the collective metabolic activities of numerous individual organisms. Most microbes use corrinoids, a structurally diverse family cofactors related to vitamin B12. Corrinoid structure influences growth microbes, yet how these responses scale community level remains unknown. Analysis metagenome-assembled genomes suggests that corrinoids are supplied by members archaeal and bacterial phyla Thermoproteota,...

Soil microbial communities impact carbon sequestration and release, biogeochemical cycling, agricultural yields. These global effects rely on metabolic interactions that modulate community composition function. However, the physicochemical taxonomic complexity of soil scarcity available isolates for phenotypic testing are significant barriers to studying interactions. Corrinoids-the vitamin B12 family cofactors-are critical metabolism, yet they synthesized by only a subset microbiome...

In soils, the first rain after a prolonged dry period represents major pulse event impacting soil microbial community function, yet we lack full understanding of genomic traits associated with response to rewetting. Genomic such as codon usage bias and genome size have been linked bacterial growth in soils—however, often through measurements culture. Here, used metagenome-assembled genomes (MAGs) 18 O-water stable isotope probing metatranscriptomics track transcription microorganisms over...

The beneficial human gut bacterium Akkermansia muciniphila provides metabolites to other members of the microbiota by breaking down host mucin, but most its metabolic functions have not been investigated. A. strain MucT is known use cobamides, vitamin B12 family cofactors with structural diversity in lower ligand. However, unable synthesize cobamides de novo, and specific forms that can be used examined. We found levels growth were nearly identical each seven tested, contrast all bacteria...

Abstract Rewetting of seasonally dry soils induces a burst microbial activity and carbon mineralization that changes nutrient availability leads to succession. Yet the functions underpin this succession are not well described. Further, it’s unclear how previous precipitation frames capacities after rewetting long these effects persist. We used isotopically-labeled water rewet annual grassland soil experienced either mean or reduced during two years, sampled at five subsequent time points....

Abstract Soil microbial communities perform critical ecosystem services through the collective metabolic activities of numerous individual organisms. Most microbes use corrinoids, a structurally diverse family cofactors related to vitamin B 12 . Corrinoid structure influences growth microbes, yet how these responses scale community level remains unknown. Analysis metagenome-assembled genomes suggests corrinoids are supplied by members archaeal and bacterial phyla Thermoproteota ,...

Abstract Candidate Phyla Radiation (CPR) bacteria and nanoarchaea populate most ecosystems, but are rarely detected in soil. We concentrated particles less than 0.2 μ m from grassland soil, enabling targeted metagenomic analysis of these organisms, which almost totally unexplored recovered a diversity CPR some sequences, no sequences other cellular organisms. The sampled include Doudnabacteria (SM2F11) Pacearchaeota, organisms not previously reported as well Saccharibacteria, Parcubacteria...

Abstract Viruses are abundant, ubiquitous members of soil communities that kill microbial cells, but how they respond to perturbation ecosystems is essentially unknown. Here, we investigated lineage-specific virus-host dynamics in grassland following “wet-up”, when resident microbes both resuscitated and lysed after a prolonged dry period. Quantitative isotope tracing, time-resolved metagenomics viromic analyses indicated held diverse low biomass reservoir virions, which only subset thrived...

ABSTRACT In soils, the first rain after a prolonged dry period greatly impacts soil microbial community function, yet we lack full understanding of genomic traits associated with response to rewetting. Genomic such as codon usage bias and genome size have been linked bacterial growth in soils—however this is often through measurements culture. Here, used metagenome-assembled genomes combination metatranscriptomics 18 O- water stable isotope probing track transcriptional activity...

Abstract The concentration of atmospheric methane continues to increase with microbial communities controlling soil-atmosphere fluxes. While there is substantial knowledge the diversity and function organisms regulating production consumption, frequency impact interactions viruses on their activity in soil unknown. Metagenomic sequencing has enabled identification linkages between hosts. However, determining host-virus through does not determine whether a virus or host are active. In this...

In drought affected ecosystems, a large portion of the annually respired CO2 from soil may occur in short period following first rain event after drought. This process, where rewetting dry results pulse CO­2, is commonly known as Birch Effect. activity influences stability and persistence carbon which, considering growing extent dryland drought-impacted have far reaching implications. It’s been shown that consumption compounds driving Effect varies temporally different...

ABSTRACT Soil microbial communities impact carbon sequestration and release, biogeochemical cycling, agricultural yields. These global effects rely on metabolic interactions that modulate community composition function. However, the physicochemical taxonomic complexity of soil scarcity available isolates for phenotypic testing are significant barriers to studying interactions. Corrinoids—the vitamin B 12 family cofactors—are critical metabolism, yet they synthesized by only a subset...

Abstract The beneficial human gut bacterium Akkermansia muciniphila provides metabolites to other members of the microbiota by breaking down host mucin, but most its metabolic functions have not been investigated. A. is known use cobamides, vitamin B 12 family cofactors with structural diversity in lower ligand, though specific cobamides it can examined. We found that growth strain Muc T was nearly identical each seven tested, contrast all bacteria studied. Unexpectedly, this promiscuity due...